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Journals / Ankara Üniversitesi Veteriner Fakültesi Dergisi / 2010 / Cilt: 57 - Sayı: 3

Koyun kazeöz lenfadenitisine karşı aşı geliştirme çalışmaları

Studies on vaccine development for ovine caseous lymphadenitis

Ziya İLHAN
Nahit YAZICIOĞLU
Mehmet AKAN
Müjgan İZGÜR
Pages
161–165
Publication Date
2022-10-12
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

When a local C. pseudotuberculosis (Pl 18) strain was used and formulated as per ml of the vaccine, 1.5x$10^8$ cfu of C. pseudotuberculosis bacterin + toxoid + 1/2 FCA of the toxoid, we obtained encouraging results regarding the potential of this vaccine to control of CLA in sheep.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Caseous lymphadenitis (CLA), which is a serious infectious disease of sheep and goats world-wide, has caused significant economic losses in Turkey in recent years.
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Key Findings & Data:SDS-PAGE revealed more than 21 bands ranging in size from 15 kDa to 194 kDa present in different concentrations in C. pseudotuberculosis isolates as determined by visual assessment of their approximate molecular masses.
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Conclusion & Impact:When a local C. pseudotuberculosis (Pl 18) strain was used and formulated as per ml of the vaccine, 1.5x$10^8$ cfu of C. pseudotuberculosis bacterin + toxoid + 1/2 FCA of the toxoid, we obtained encouraging results regarding the potential of this vaccine to control of CLA in sheep.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Caseous lymphadenitis (CLA), which is a serious infectious disease of sheep and goats world-wide, has caused significant economic losses in Turkey in recent years. In this study, it was aimed to identify major antigens of Corynebacterium pseudotuberculosis strains isolated in Turkey and to evaluate the efficiency of the vaccine prepared from local strains of C. pseudotuberculosis against bacterial challenge in lambs. C. pseudotuberculosis strains were isolated from six different flocks in Turkey and were classified according to their similarities by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDSPAGE). SDS-PAGE revealed more than 21 bands ranging in size from 15 kDa to 194 kDa present in different concentrations in C. pseudotuberculosis isolates as determined by visual assessment of their approximate molecular masses. Lambs were vaccinated with a bacterin + toxoid + Freund’s complete adjuvant (FCA) vaccine, and the vaccine efficiency against bacteriological challenge was evaluated. When a local C. pseudotuberculosis (Pl 18) strain was used and formulated as per ml of the vaccine, 1.5x$10^8$ cfu of C. pseudotuberculosis bacterin + toxoid + 1/2 FCA of the toxoid, we obtained encouraging results regarding the potential of this vaccine to control of CLA in sheep.

Özet

Koyun ve keçilerin önemli bir infeksiyonu olan kazeöz lenfadenitis (KLA), son zamanlarda Türkiye’de önemli ekonomik kayıplara neden olmaktadır. Bu çalışmada, Türkiye’den izole edilen farklı Corynebacterium pseudotuberculosis suşlarının major antijenlerinin belirlenmesi ve lokal suşlardan hazırlanan aşıların, toklularda eprüvasyona karşı koruyuculuğunun ortaya konulması amaçlandı. Altı farklı sürüden izole edilen C. pseudotuberculosis suşları, sodyum dodesil sulfat-poliakrilamid jel elektroforezis (SDS-PAGE) ile analiz edildiğinde, molekül ağırlıkları 15-194 kDa arasında değişen 21 bant tespit edildi. Aşı denemeleri toklularda gerçekleştirildi ve hayvanlar, C. pseudotuberculosis’in bakterin + toksoid + Freund’un komple adjuvantı (FKA)’nı içeren aşı ile aşılandı. Sonuç olarak, lokal C. pseudotuberculosis (Pl 18) suşunun, 1.5x$10^8$ cfu/ml bakterin + toxoid + 1/2 FKA’tı şeklinde formüle edilip, aşı olarak kullanılmasının, koyunları KLA’e karşı korumada başarılı olduğu tespit edildi.

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Cite This Article

İLHAN, Z., YAZICIOĞLU, N., AKAN, M., İZGÜR, M. (2022). Koyun kazeöz lenfadenitisine karşı aşı geliştirme çalışmaları. Ankara Üniversitesi Veteriner Fakültesi Dergisi, Cilt: 57 - Sayı: 3, 161–165. https://ajindex.com/articles/445017

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References (14)

  1. Batey RG (1986): Frequency and consequence of caseous lymphadenitis in sheep and lambs slaughtered and a western Australian abattoir. Am J Vet Res, 47, 482-485
  2. Braithwaite CE, Smith EE, Songer JG, Reine AH (1993): Characterization of detergent-soluble proteins of Corynebacterium pseudotuberculosis. Vet Microbiol, 38, 59-70
  3. Brogden KA, Chedid L, Cutlip RC, Lehmkuhl HD, Sacks J (1990): Effect of muramyl dipeptide on immnunogenicity of Corynebacterium pseudotuberculosis whole-cell vaccines in mice and lambs. Am J Vet Res, 51, 200-202
  4. Brogden KA, Cutlip RC, Lehmkuhl HD (1984): Comparison of protection induced in lambs by Corynebacterium pseudotuberculosis whole cell and cell wall vaccines. Am J Vet Res, 45, 2393-2395
  5. Brown CC, Olander HJ (1987): Caseous lymphadenitis of goats and sheep: A review. Vet Bull, 57, 1-11
  6. Brown CC, Olander HJ, Biberstein EL, Morse SM (1986): Use of a toxoid vaccine to protect goats against intradermal challenge exposure to Corynebacterium pseudotuberculosis. Am J Vet Res, 47, 1116-1119
  7. Burrell DH (1980): A haemolysis inhibition test for detection of antibody to Corynebacterium ovis exotoxin. Res Vet Sci, 28, 190-194
  8. Cummins CS (1962): Chemical composition and antigenic structure of cell walls of Corynebacterium, Mycobacterium, Actinomyces and Athrobacter. J Gen Microbiol, 28, 3505-3510
  9. Eggleton DG, Doidge CV, Middleton HD, Minty DW (1991): Immunisation against ovine caseous lymphadenitis: Efficacy of monocomponent Corynebacterium pseudotuberculosis toxoid vaccine and combined clostridialcorynebacterial vaccines. Aust Vet J, 68, 320-321
  10. Ellis JA, Hawk DA, Mills KW, Pratt DL (1991): Antigen specificity and activity of ovine antibodies induced by immunization with Corynebacterium pseudotuberculosis culture filtrate. Vet Immunol Immunopathol, 28, 303-316
  11. Izgür M, Akan M, Ilhan Z (1999): Koyunlarda kazeöz lenfadenitis olgularından izole edilen etkenler. Ankara Univ Vet Fak Derg, 46, 43-50
  12. Keskintepe H (1976): Stabilization of Corynebacterium ovis antigens for serum agglutination test. Fırat Üniv Vet Fak Derg, 3, 84-93
  13. LeaMaster BR, Shen DT, Gorham JR, Leathers VW, Wells HD (1987): Efficacy of Corynebacterium pseudotuberculosis bacterin for the immunological protection of sheep against development of caseous lymphadenitis. Am J Vet Res, 48, 869-872
  14. Laemmli UK (1970): Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, 227, 680-685