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Journals / Ankara Üniversitesi Veteriner Fakültesi Dergisi / 2010 / Cilt: 57 - Sayı: 2

Bir süt sığırcılığı işletmesinde metritisli ineklerde BoHV-4 ün varlığının araştırılması

The investigation of the presence of bovine herpesvirus type 4 (BoHV-4) in cows with metritis in a dairy herd

Tuba Çigdem OGUZOGLU
Seval Bilge DAGALP
Veysel Soydal ATASEVEN
Volkan YILMAZ
Elvin GUNGOR
Ayşe Başak DEMIR
Dilek Pınar MUZ
Feray ALKAN
Pages
87–91
Publication Date
2022-10-12
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

In this study, the presence of BoHV-4 was determined in cows with metritis in post-partum period in a dairy herd.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:To investigate the occurrence of bovine herpesvirus type 4 (BoHV-4) infection in a dairy herd including cows with metritis, various methods were used for the diagnosis BoHV-4 in vaginal discharge samples.
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Key Findings & Data:Blood serum samples were obtained from 368 cows for serodiagnosis of BoHV-4 infection.
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Conclusion & Impact:In this study, the presence of BoHV-4 was determined in cows with metritis in post-partum period in a dairy herd.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

To investigate the occurrence of bovine herpesvirus type 4 (BoHV-4) infection in a dairy herd including cows with metritis, various methods were used for the diagnosis BoHV-4 in vaginal discharge samples. Blood serum samples were obtained from 368 cows for serodiagnosis of BoHV-4 infection. Of 368 serum samples, 256 (69.6%) were found positive for BoHV- 4 specific antibodies. For the virological study, vaginal discharge samples of 55 cows with metritis were used and tested for BoHV-4 using polymerase chain reaction (PCR), virus isolation and immunofluorescence techniques. Twenty-nine percent (16/55) of the vaginal discharge samples obtained from cows with metritis were found positive for BoHV-4 DNA by PCR. In this study, the presence of BoHV-4 was determined in cows with metritis in post-partum period in a dairy herd.

Özet

Metritis olgularının gözlendiği bir sütçü sığır sürüsünde, örneklenen vajinal svaplarda farklı tanı yöntemleriyle BoHV-4 infeksiyonunun varlığı araştırıldı. BoHV-4 ün serolojik tanısı amacıyla 169 adedi metritis semptomları gösteren, 199 adedi ise metritisli hayvanlarla birlikte barındırılan sağlıklı görünümlü hayvanlar olmak üzere toplam 368 adet sığırdan kan serum örneği alındı. Virolojik kontrol amacıyla, metritisli 55 adet inekten alınan vajinal svap örnekleri polimeraz zincir reaksiyonu, virus izolasyonu ve immunfloresan tekniği ile BoHV-4 yönünden araştırıldı. 368 serum örneğinin 256 adedi (%69.6) BoHV-4 spesifik antikorları yönünden pozitif bulundu. Seropozitiflik oranları, metritisli ve metritisli hayvanlarla birlikte barındırılan sağlıklı görünümlü hayvanlarda sırasıyla, %69.8 ve %69 olarak tespit edildi. Metritisli hayvanlardan alınan vajinal svap örneklerinin %29 (16/55)'unun BoHV-4 DNA'sı yönünden PCR tekniği ile pozitif olduğu saptandı. Bu çalışmada, bir süt sığırcılığı işletmesinde bulunan doğum sonrası dönemdeki metritis problemli ineklerde BoHV-4 ün varlığı belirlenmiştir.

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Cite This Article

OGUZOGLU, T. Ç., DAGALP, S. B., ATASEVEN, V. S., YILMAZ, V., GUNGOR, E., DEMIR, A. B., MUZ, D. P., ALKAN, F. (2022). Bir süt sığırcılığı işletmesinde metritisli ineklerde BoHV-4 ün varlığının araştırılması. Ankara Üniversitesi Veteriner Fakültesi Dergisi, Cilt: 57 - Sayı: 2, 87–91. https://ajindex.com/articles/444978

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