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Journals / Ankara Üniversitesi Veteriner Fakültesi Dergisi / 2016 / Cilt: 63 - Sayı: 2

Anahtar sözcükler: Balık, balık ürünleri, histamin, metot, validasyon. Comparison of high pressure liquid chromatography methods in fish and fish products by using o-phthaldialdehyde and benzoyl chloride derivatization for the determination of histamine levels

Balık ve balık ürünlerinde histamin düzeylerinin tespiti için o-fitaldialdehit ve benzoil klorür türevlendirmesi kullanılan yüksek performans sıvı kromatografisi yöntemlerinin karşılaştırılması

Deniz TURAN
Ulvi Reha FİDANCI
Pages
93–101
Publication Date
2022-10-12
DOI
—
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

There was a significant difference between for both methods in t-test (p

📌 3 Key Takeaways

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Objective & Hypothesis:Summary: In this study, by the parameters of validation a comparison of two high pressure liquid chromatography (HPLC) methods one of which utilizes coupled the FLD and the o-phthaldialdehyde derivatization method of Antoine et al. (1999) versus DAD coupled, benzoyl chloride derivatization method with a modification of Özdestan and Üren (2009) were made for the selection of sensitive and safe assay for the determination of histamine.
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Key Findings & Data:For the other validation parameters, recovery, accuracy, precision and comparison of methods a certified reference material; FAPAS T2775QC were used.
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Conclusion & Impact:There was a significant difference between for both methods in t-test (p
🔬Design:Research Article
📍Scope:2009 Verileri
⚙️Variables:Domain Parameters

Abstract

Summary: In this study, by the parameters of validation a comparison of two high pressure liquid chromatography (HPLC) methods one of which utilizes coupled the FLD and the o-phthaldialdehyde derivatization method of Antoine et al. (1999) versus DAD coupled, benzoyl chloride derivatization method with a modification of Özdestan and Üren (2009) were made for the selection of sensitive and safe assay for the determination of histamine. Alive horse mackerels caught from the sea of Marmara in Kucukcekmece Istanbul were used for the limit of detection studies. For the other validation parameters, recovery, accuracy, precision and comparison of methods a certified reference material; FAPAS T2775QC were used. Results of the analyses indicated that the FLD coupled; o-phthaldialdehyde derivatization method the limit of quantification (LOQ) and recovery were 1.38 ppm and 100% respectively; and for DAD coupled; benzoyl chloride derivatization modified method these values were 10.66 ppm and 88% respectively. There was a significant difference between for both methods in t-test (p

Özet

Özet: Çalışmada histamin düzeylerinin hassas ve güvenilir bir şekilde tespit edilmesi için, yüksek performans sıvı kromatogra- fisi (HPLC) cihazı kullanılarak Antoine ve ark. (1999) tarafından önerilen floresans dedektör (FLD) ve o-fitaldialdehit türevlendir- mesi kullanılan yöntem ile Özdestan ve Üren (2009) tarafından geliştirilen fotodiyot array dedektörü (DAD) ve benzoil klorür türev- lendirmesinin kullanıldığı yöntem validasyon parametreleri kullanılarak karşılaştırılmıştır. Teşhis limiti çalışmaları için İstanbulda Küçükçekmece sahilinden tutulan canlı istavrit balığı, geri kazanım, doğruluk, kesinlik parametreleri ve yöntemler arası karşılaştırma için ise sertifikalı referans materyal olan FAPAS T2775QC kullanılmıştır. Yapılan analizler sonucunda FLD ve o-fitaldialdehit türev- lendirmesi ile elde edilen ölçüm limiti (LOQ) 1.38 ppm, geri kazanım %100; benzoil klorür yönteminde bu parametreler 10.66 ppm ve % 88dir. Her iki yöntemde de yapılan t-testi hesaplamalarına göre iki yöntem arasındaki fark anlamlıdır (p

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Cite This Article

TURAN, D., FİDANCI, U. R. (2022). Anahtar sözcükler: Balık, balık ürünleri, histamin, metot, validasyon. Comparison of high pressure liquid chromatography methods in fish and fish products by using o-phthaldialdehyde and benzoyl chloride derivatization for the determination of histamine levels. Ankara Üniversitesi Veteriner Fakültesi Dergisi, Cilt: 63 - Sayı: 2, 93–101. https://ajindex.com/articles/444933

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