Higher survival rates were observed in shrimp fed probiotic diets as compared with the control (P
📌 3 Key Takeaways
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Objective & Hypothesis:In the present study, Lactobacillus bulgaricus E20 isolated from the gut contents of farm-reared white shrimp, Litopenaeus vannamei, after determination of its characteristics using biochemical, molecular and probiotical examinations, was used in the diet of white shrimp to evaluate the survival rate, immune status, and disease resistance.
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Key Findings & Data:L. bulgaricus was administered at two different doses 1×107 (T1) and 1×109 (T2) CFU g_1 feed to shrimp for 30 days.
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Conclusion & Impact:Higher survival rates were observed in shrimp fed probiotic diets as compared with the control (P
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters
Abstract
In the present study, Lactobacillus bulgaricus E20 isolated from the gut contents of farm-reared white shrimp, Litopenaeus vannamei, after determination of its characteristics using biochemical, molecular and probiotical examinations, was used in the diet of white shrimp to evaluate the survival rate, immune status, and disease resistance. L. bulgaricus was administered at two different doses 1×107 (T1) and 1×109 (T2) CFU g_1 feed to shrimp for 30 days. A control group was also included with normal feed. The survival rate, total hemocyte counts, phenoloxidase activity, respiratory burst, gut lactic acid bacteria and gut total bacteria were evaluated at the end of trial and after challenging shrimp with Vibrio parahaemolyticus. Higher survival rates were observed in shrimp fed probiotic diets as compared with the control (P
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Cite This Article
AHMADI, S., ROOMIANI, L., GHAENI, M. (2022). Immune response and disease resistance in the white shrimp, Litopenaeus vannamei induced by potential probiotic Lactobacillus bulgaricus. Ankara Üniversitesi Veteriner Fakültesi Dergisi, Cilt: 65 - Sayı: 3, 324–329. https://ajindex.com/articles/444904
Ziaei-Nejad S, Rezaei MH, Takami GA, et al. (2006): The effect of Bacillus spp. bacteria used as probiotics on digestive enzyme activity, survival and growth in the India white shrimp Fenneropenaeus indicus. Aquaculture, 252, 516-24.
You XX, Su YQ, Mao Y, et al. (2010): Effect of high water temperature on mortality, immune response and viral replication of WSSV-infected Marsupenaeus japonicas juveniles and adults. Aquaculture, 305, 133-137.
Wang Y (2007): Effect of probiotics on growth performance and digestive enzyme activity of the shrimp Penaeus vannamei. Aquaculture, 269, 259-264.
Verschuere L, Rombaut G, Sorgeloos P, et al. (2000): Probiotic bacteria as biological control agents in aquaculture. Microbiol Mol Biol Rev, 64, 655-671.
Vargas-Albores F, Gúzman MA, Ochoa JL (1993): An anticoagulant solution for haemolymph collection and prophenoloxidase studies of penaeid shrimp (Penaeus californiensis). Comp Biochem Physiol, 106A, 299-303.
Tseng DY, Ho PL, Huang SY, et al. (2009): Enhancement of immunity and disease resistance in the white shrimp, Litopenaeus vannamei, by the probiotic, Bacillus subtilis E20. Fish Shell Immunol, 26, 339-344.
Tendencia EA, De la Peña LD (2002): Level and percentage recovery of resistance to oxytetracycline and oxolinic acid of bacteria from shrimp ponds. Aquaculture, 213, 1-13.
Sung HH, Yang YL, Song YL (1996): Enhancement of microbicidal activity in the tiger shrimp Penaeus monodon via immunostimulation. J Crustacean Biol, 16, 278-284.
Soto-Rodriguez S, Armenta M, Gomez-Gil B (2006): Effects of enrofloxacin and florfenicol on survival and bacterial population in an experimental infection with luminescent Vibrio campbellii in shrimp larvae of Litopenaeus vannamei. Aquaculture, 255, 48-54.
Smith JV, Soderhall K (1983): Induction of degranulation and lysis of haemocytes in the fresh water crayfish, Astacus astacus by components of the prophenoloxidase activating system in vitro. Cell Tissue Res, 233, 295-303.
Sivakumar N, Sundararaman M, Selvakumar G (2012): Probiotic effect of Lactobacillus acidophilus against vibriosis in juvenile shrimp (Penaeus monodon). Afr J Biotechnol, 11, 15811-18.
Singh SK, Tiwari VK, Chadha NK, et al. (2013): Effect of Bacillus circulans and fructooligosaccharide supplementation on growth and haemato-immunological function of Labeo rohita (Hamilton, 1822) fingerlings exposed to sub-lethal nitrite stress. Israeli J Aquacult - Bamidgeh, 64, 1-11.
Saurabh S, Sahoo PK (2008): Lysozyme: An important defe