Journals / Ankara Üniversitesi Veteriner Fakültesi Dergisi / 2019 / Cilt: 66 - Sayı: 1

Comparison of the volume fraction values of grey matter on the cervical enlargement of the spinal cord in chicken and quail

Tavuk ve bıldırcında medulla spinalis’in intumescentia cervicalis’inde gri cevher hacim fraksiyon değerlerinin karşılaştırılması

Pages
1–6
Publication Date
2022-10-12
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

On the other hand, the Cavalieri principle was considered as an unbiased, accurate and effective method for volume and volume fraction estimations.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:The aim of this study was to estimate and compare volume fraction values of grey matter (substantia grisea) in the cervical enlargement (intumescentia cervicalis) using stereological methods for two different types of birds.
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Key Findings & Data:The tissues cut into serial sections of 5 μm thickness were selected according to systematic random sampling and stained with cresyl violet.
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Conclusion & Impact:On the other hand, the Cavalieri principle was considered as an unbiased, accurate and effective method for volume and volume fraction estimations.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

The aim of this study was to estimate and compare volume fraction values of grey matter (substantia grisea) in the cervical enlargement (intumescentia cervicalis) using stereological methods for two different types of birds. Ten pairs of quails and chickens were included in this study as one of them is flying and the other one is flightless bird species. The tissues cut into serial sections of 5 μm thickness were selected according to systematic random sampling and stained with cresyl violet. Volume and volume fractions were calculated in accordance with the Cavalieri principle. Student’s t-test was used to evaluate volume fraction differences among the species. The volume fractions of grey matter in cervical enlargement of chicken and quail were 0.277 ± 0.005 and 0.346 ± 0.014 (Mean ± Standart Deviation), respectively (p < 0.001). There was a significant difference between chicken and quail cervical enlargements in terms of volume fraction values of grey matter. On the other hand, the Cavalieri principle was considered as an unbiased, accurate and effective method for volume and volume fraction estimations.

Özet

Bu çalışmanın amacı, stereolojik yöntemler kullanarak iki farklı kuş türünün cervical genişlemesindeki (intumescentia cervicalis) gri cevherin (substantia grisea) hacim fraksiyon değerlerini hesaplamak ve karşılaştırmaktır. Araştırmaya biri uçabilen, diğeri uçamayan kanatlı türü olarak on adet bıldırcın ve on adet tavuk dahil edildi. Dokulardan 5 μm kalınlığında alınan seri kesitler, sistematik rastgele örnekleme kuralına göre seçildi ve cresyl violet ile boyandı. Hacim ve hacim fraksiyon değerleri Cavalieri prensibine göre hesaplandı. Türler arasındaki hacim fraksiyon farklılığını değerlendirmek için student’s t testi kullanıldı. Tavuk ve bıldırcının cervical genişlemedeki gri cevher hacim fraksiyon değerleri sırasıyla, 0.277 ± 0.005 and 0.346 ± 0.014 (Ortalama ± Standart Sapma) olarak belirlendi (p < 0.001). Tavuk ve bıldırcının cervical genişlemelerinin gri cevherine ait hacim fraksiyon değerleri arasında önemli bir fark bulundu. Bunun yanı sıra, hacim ve hacim fraksiyonu tahminleri için Cavalieri prensibinin tarafsız, doğru ve etkili bir yöntem olduğu görüldü.

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Cite This Article

EKİM, O., ÇAKIR, A., ÖZEN, D., OTO, Ç., AHLAT, O., BAKICI, C. (2022). Comparison of the volume fraction values of grey matter on the cervical enlargement of the spinal cord in chicken and quail. Ankara Üniversitesi Veteriner Fakültesi Dergisi, Cilt: 66 - Sayı: 1, 1–6. https://ajindex.com/articles/444891

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References (17)

  1. Bahar S, Bolat D, Selçuk ML (2013): The segmental morphometric properties of the horse cervical spinal cord: A study of cadaver. Scientific World J, 2013, 1-9
  2. Bancroft JD, Gamble M (2002): Theory and Practice of Histological Techniques. Livingstone Publishing, Edinburgh, England
  3. Baumel JJ (1975): Aves Nervous System. 437-448. In: CE Rosenbaum, NG Ghoshal, D Hillmann (Ed). Sisson’s and Grossman’s The Anatomy of The Domestic Animals. Philadelphia, Saunder, USA
  4. Bolat D, Tıpırdamaz S (2012): The use of optical fractionator to estimate the total number of ependymal cells of the spinal cord in leghorn. Kafkas Univ Vet Fak Derg, 18, 129-133
  5. Bolat D, Bahar S, Sur E, et al (2012): Selective gray and white matter staining of the horse spinal cord. Kafkas Univ Vet Fak Derg, 18, 249-254
  6. Cahill MM, Kett MM, Mccausland JE, et al (1996): Glomerular stereology: Why, what and how to measure glomerular structure. Nephrology, 2, 305-313
  7. Dinç G, Yılmaz S (2002): Sinir sistemi. 157-185. In: N Dursun, (Ed). Evcil Kuşların Anatomisi. Medisan Yayınevi, Ankara, Türkiye
  8. Garcia-Finana M, Cruz-Orive LM, Mackay CE, et al (2003): Comparison of MR imaging against physical sectioning to estimate the volume of human cerebral compartments. Neuroimage, 18, 505-516. prediction. J Microsc, 147, 229-263
  9. Gundersen HJG, Jensen EB (1987): The efficiency of systematic sampling in stereology and its prediction. J Microsc, 147, 229-263
  10. Hazıroğlu RM, Orhan İÖ, Yıldız D, et al (2001): Morphology of the spinal cord in the chicken, duck and pigeon. Turk J Vet Anim Sci, 25, 913-920
  11. Howard CV, Reed MG (2005): Unbiased Stereology. Garland Science, Cornwall, England
  12. Hsu PK, Huang HC, Hsieh CC, et al (2007): Effect of formalin fixation on tumor size determination in stage I non-small cell lung cancer. Ann Thorac Surg, 84, 1825-1829
  13. Jonmarker S, Valdman A, Lindberg A, et al (2006): Tissue shrinkage after fixation with formalin injection of prostatectomy specimens. Virchows Arch, 449, 297-301
  14. Ko HY, Park JH, Shin YB, et al (2004): Gross quantitative measurements of spinal cord segments in human. Spinal Cord, 42, 35-40
  15. König HE, Liebich HG, Cerveny C (2004): Nervous system. 465-536. In: HE König, HG Liebich, (Ed). Veterinary Anatomy of Domestic Mammals Textbook and Colour Atlas. Schattauer, Stuttgart, Germany
  16. Kristiansen SL, Nyengaard JR (2012): Digital stereology in neuropathology. Acta Path Micro Im A, 120, 327-340
  17. Lin JY, Peng B, Yang