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Journals / Ankara Üniversitesi Veteriner Fakültesi Dergisi / 2013 / Cilt: 60 - Sayı: 1

Investigation of some toxins genes and methicillin resistance gene in Staphylococcus aureus isolates from cows with subclinical mastitis

Subklinik mastitisli inek sütlerinden izole edilen Staphylococcus aureus izolatlarında bazı toksin genleri ve metisilin direnç geninin araştırılması

Nilgün ÜNAL
Pages
21–26
Publication Date
2022-10-12
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

This situtiation could be a potential risk factor for public health.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:The aim of this study was to investigate the genes of some staphylococcal enterotoxins and Panton-Valentine leukocidin and methicillin resistance of Staphylococcus aureus strains isolated from subclinical bovine mastitis.
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Key Findings & Data:In this study, 60 S. aureus isolates were used and the genes were detected by PCR. seg-sei, sej, sec and seh genes were detected in eight (13.33%) isolates, six (10.00%) isolates, four (6.67%) isolates and three (5.00%) isolates, respectively.
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Conclusion & Impact:This situtiation could be a potential risk factor for public health.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

The aim of this study was to investigate the genes of some staphylococcal enterotoxins and Panton-Valentine leukocidin and methicillin resistance of Staphylococcus aureus strains isolated from subclinical bovine mastitis. In this study, 60 S. aureus isolates were used and the genes were detected by PCR. seg-sei, sej, sec and seh genes were detected in eight (13.33%) isolates, six (10.00%) isolates, four (6.67%) isolates and three (5.00%) isolates, respectively. Sixteen (26.67%) isolates were found to harbor one or more toxin genes. None of the investigated isolates harbored sea, seb, sed, see, and tsst genes, while four (6.6%) isolates and two (3.30%) isolates had Panton-Valentine leukocidin gene and mecA gene, respectively. As a conclusion, it was determined that S. aureus strains isolated from cows with subclinical mastitis could produce enterotoxin and harbor mecA gene. This situtiation could be a potential risk factor for public health.

Özet

Bu çalışma subklinik mastitisli inek sütlerinden izole edilen Staphylococcus aureus izolatlarında bazı stafilokokal enterotoksin, Panton-Valentine lökosidin genleriyle metisilin direnç geninin araştırılması amacıyla yapılmıştır. Araştırmada 60 S. aureus izolatı kullanılmış ve genlerin belirlenmesinde polimeraz zincir reaksiyonundan yararlanılmıştır. İzolatların sekizinde (% 13.33) seg-sei, altısında (% 10.00) sej, dördünde (% 6.67) sec ve üçünde (% 5.00) ise seh geni belirlenmiştir. Toplam on altı (% 26.67) izolatta bir veya daha fazla enterotoksin geni tespit edilmiştir. İncelenen izolatlarda enterotoksin genlerinden sea, seb, sed, see ve tsst belirlenemezken dördünde (% 6.6) Panton-Valentine lökosidin geni ve ikisinde (% 3.30) metisilin direnç geni belirlenmiştir. Sonuç olarak subklinik mastitisli sütlerde bulunan S. aureus izolatlarının enterotoksin üretebilecekleri ve metisilin direnç geni taşıyabilecekleri belirlenmiştir. Bu durum halk sağlığı açısından önemli bir risk oluşturmaktadır.

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Cite This Article

ÜNAL, N. (2022). Investigation of some toxins genes and methicillin resistance gene in Staphylococcus aureus isolates from cows with subclinical mastitis. Ankara Üniversitesi Veteriner Fakültesi Dergisi, Cilt: 60 - Sayı: 1, 21–26. https://ajindex.com/articles/444857

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References (14)

  1. Akan M (2006): Staphylococcus infeksiyonları. 15-29 In: Aydın N, Paracıklıoğlu J (Ed), Veteriner Mikrobiyoloji (Bakteriyel Hastalıklar). İlke-Emek yayınları, Ankara
  2. Balaban N, Rasooly A (2000): Staphylococcal enterotoxins. Inter J Food Microbiol, 61, 1-10
  3. Choi SM, Kim SH, Kim HJ, Lee DG, Choi JH, Yoo JH, Kang JH, Shin WS, Kang MW (2003): Multiplex PCR for detection of genes encoding aminoglicoside modifying enzymes and methicillin resistance among Staphylococcus species. J Korean Med Sci, 18, 631-636
  4. Ciftci A, Findik A, Onuk EE, Savasan S (2009): Detection of methicillin resistance and slime factor production of Staphylococcus aureus in bovine mastitis. Braz J Microbiol, 40, 254-261
  5. Dinges MM, Orwin PM, Schlievert PM (2000): Exotoxins of Staphylococcus aureus. Clin Microbiol Rev, 13, 16-34
  6. Erol İ, İşeri Ö (2004): Stafilokokal enterotoksinler. Ankara Üniv Vet Fak Derg, 51, 239-245
  7. Freitas MFL, Luz IS, Silveira-Filho VM, Junior JWP, Stamford TLM, Mota RA, Sena MJ, Almeida AMP, Balbino VQ, Leal-Baibino TC (2008): Staphylococcal toxin genes in strains isolated from cows with subclinical mastitis. Pesquisa Vet Brasileira, 28, 617-621
  8. Günaydın B, Aslantaş Ö, Demir C (2011): Detection of superantigenic toxin genes in Staphylococcus aureus strains from subclinical bovine mastitis. Trop Anim Health Product, 43, 1633-1637
  9. Hazımoğlu Ş (2011): Staphylococcus aureus suşlarında Panton Valentine Lökosidin (PVL) genlerinin araştırılması. ADÜ. Sağlık Bil Enst Doktora Tezi, Aydın
  10. İnegöl E, Türkyılmaz S (2012): Determination of SCCmec types in methicillin resistant staphylococci isolated from cows and farm workers. Ankara Üniv Vet Fak Derg, 59, 89-93
  11. Karahan M, Açık MN, Çetinkaya B (2009): Investigation of toxin genes by polymerase chain reaction in Staphylococcus aureus strains isolated from bovine mastitis in Turkey. Foodborne Pathog Dis, 6, 1029-1035
  12. Lee JH (2003): Methicillin (oxacillin)-resistant Staphylococcus aureus strains isolated from major food animals and their potential transmission to humans. Appl Environ Microbiol, 69, 6489–6494
  13. Lina G, Piemont Y, Godail-Gamot F, Bes M, Peter M, Gauduchon V, Vandenesch F, Etienne J (1999): Involvement of Panton-Valentine leukocidin–producing Staphylococcus aureus in primary skin infections and pneumonia. Clin Infec Dis, 29,1128–1132
  14. Løvseth A, Loncarevic S, Berdal KG (2004): Modified multiplex PCR method for detection of pyrogenic enterotoxin genes in Staphylococcal i