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Journals / Ankara Üniversitesi Veteriner Fakültesi Dergisi / 2013 / Cilt: 60 - Sayı: 4

Tarla farelerinde (Microtus socialis) Bartonella Spp. varlığının kültür ve PCR yöntemleriyle belirlenmesi

Detection ofBartonellaspp.in field mice (Microtus socialis) by culture and PCR

Hülya ŞİMŞEK
Alper KARAGÖZ
Mustafa ERTEK
Selçuk KILIÇ
Müge TANER
Rıza DURMAZ
Bekir ÇELEBİ
Pages
235–239
Publication Date
2022-10-12
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

This study is the first report for the presence of Bartonella spp. in rodents in Turkey.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:The species within the genus Bartonella are intracellular bacteria causing long-lasting bacteremia in humans and animals.
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Key Findings & Data:In this study, Bartonella spp. in 42 field mice, (Microtus socialis), captured from one spot in Central Anatolia Region were investigated by culture and molecular methods.
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Conclusion & Impact:This study is the first report for the presence of Bartonella spp. in rodents in Turkey.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

The species within the genus Bartonella are intracellular bacteria causing long-lasting bacteremia in humans and animals. Eleven Bartonella species isolated from animals have zoonotic characteristics. The four of zoonotic Bartonella species have been reported in rodents. In this study, Bartonella spp. in 42 field mice, (Microtus socialis), captured from one spot in Central Anatolia Region were investigated by culture and molecular methods. The positivity of Bartonella was 16.6% (7/42) by culture, and 57.1% (24/42) by PCR (Polymerase Chain Reaction) from liver tissues of field mice. The citrate synthesis gene regions (gltA) of the isolated seven strains were identified by DNA sequencing analysis. It was determined that five isolates were Bartonella taylorii, and two isolates were Bartonella grahamii which are zoonotic. This study is the first report for the presence of Bartonella spp. in rodents in Turkey.

Özet

Bartonella genusundaki türler insanlarda ve hayvanlarda uzun süreli bakteriyemiye neden olan hücre içi bakterilerdir. Hayvanlardan izole edilen onbir Bartonella türü zoonoz karakterdedir. Zoonoz Bartonella türlerinden dördü rodentlerde bildirilmiştir. Bu çalışmada Orta Anadolu Bölgesinde bir odaktan yakalanan 42 Microtus socialis türü tarla faresinde kültür ve moleküler yöntemlerle Bartonella spp. araştırıldı. Tarla farelerinde, karaciğer dokusundan kültür ile % 16.6 (7/42), PCR (Polimeraz Zincir Reaksiyonu) ile % 57,1 (24/42) Bartonella pozitifliği tespit edildi. Kültürde izole edilen yedi izolatın, sitrat sentez gen bölgesi (gltA) DNA dizi analizi ile tür tanımlamaları yapıldı. İzolatlardan beşi Bartonella taylorii, ikisi zoonotik bir tür olan Bartonella grahamii olarak belirlendi. Bu çalışma ile ülkemizde rodentlerde ilk defa Bartonella türlerinin varlığı bildirilmektedir.

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Cite This Article

ŞİMŞEK, H., KARAGÖZ, A., ERTEK, M., KILIÇ, S., TANER, M., DURMAZ, R., ÇELEBİ, B. (2022). Tarla farelerinde (Microtus socialis) Bartonella Spp. varlığının kültür ve PCR yöntemleriyle belirlenmesi. Ankara Üniversitesi Veteriner Fakültesi Dergisi, Cilt: 60 - Sayı: 4, 235–239. https://ajindex.com/articles/444854

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