Journals / Ankara Üniversitesi Veteriner Fakültesi Dergisi / 2014 / Cilt: 61 - Sayı: 3

Kilis ve Halep keçilerinde ELISA ve Touchdown-PCR ile Q Humması nın araştırılması

Investigation of Q Fever in Kilis and Shamil goatsby ELISA and Touchdown-PCR

Pages
161–165
Publication Date
2022-10-12
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

The province of Kilis localized in South-East Anatolian Region of Turkey was decided to be a risky region for Q fever.

📌 3 Key Takeaways

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Objective & Hypothesis:This study was carried out on Kilis and Shamil goats to determine the different stages of Q fever-chronic and acute- in serum samples by indirect ELISA and in blood samples by touchdown-PCR, respectively.
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Key Findings & Data:A total of 92 serum samples comprising 46 Kilis and 46 Shamil goats was examined and 7.60%, 4.34% and 10.8% seropositivity were determined for total serum samples, Kilis and Shamil goat serum samples, respectively.
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Conclusion & Impact:The province of Kilis localized in South-East Anatolian Region of Turkey was decided to be a risky region for Q fever.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

This study was carried out on Kilis and Shamil goats to determine the different stages of Q fever-chronic and acute- in serum samples by indirect ELISA and in blood samples by touchdown-PCR, respectively. A total of 92 serum samples comprising 46 Kilis and 46 Shamil goats was examined and 7.60%, 4.34% and 10.8% seropositivity were determined for total serum samples, Kilis and Shamil goat serum samples, respectively. Of the examined 92 blood samples belonging to 46 Kilis and 46 Shamil goats, none of them yielded 687 bp PCR products by touchdown-PCR. The positive ELISA titers were thought to be the evidence of previous infection. The province of Kilis localized in South-East Anatolian Region of Turkey was decided to be a risky region for Q fever.

Özet

Bu çalışma, Kilis ve Halep keçilerinde, Q Hummasının farklı dönemlerini -kronik ve akut- sırasıyla; serum örneklerinde indirek ELISA, kan örneklerinde touchdown-PCR ile ortaya koymak için planlanmıştır. Kırkaltı Kilis ve 46 Halep keçisinden oluşan toplam 92 serum örneği incelendi ve toplam serum örnekleri, Kilis ve Halep keçi serumlarında seropoziflik sırasıyla, 7.60%, 4.34% ve 10.8% bulundu. Kırkaltı Kilis ve 46 Halep keçisine ait toplam 92 kan örneğinin, hiçbirinde touchdown-PCR ile 687 bplık PCR ürünü tespit edilmedi. Pozitif ELISA titrelerinin daha once geçirilen infeksiyona kanıt olduğu düşünüldü. Türkiyede Güneydoğu Anadolu Bölgesinde lokalize olan Kilis ilinin Q fever yönünden riskli bir bölge olduğuna karar verildi.

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Cite This Article

DALKILIÇ, B., GÜNAYDIN, E., PEKKAYA, S., MÜŞTAK, H. K. (2022). Kilis ve Halep keçilerinde ELISA ve Touchdown-PCR ile Q Humması nın araştırılması. Ankara Üniversitesi Veteriner Fakültesi Dergisi, Cilt: 61 - Sayı: 3, 161–165. https://ajindex.com/articles/444811

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