SavedAdd your journal

Journals / Ankara Üniversitesi Veteriner Fakültesi Dergisi / 2015 / Cilt: 62 - Sayı: 1

Anadolu mandalarında testiküler morfoloji ve dondurulmuş epididimal spermanın in vitro değerlendirilmesi

Testicular morphology and in vitro evaluation of frozen epididymal sperm of Anatolian buffalo[*]

Eser AKAL
Murat SELCUK
Pages
51–55
Publication Date
2022-10-12
DOI
—
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

There was no significant difference between fresh and frozen-thawed sperm samples for progressive sperm motility and head-acrosome anomalies of spermatozoa.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Epididymal sperm may be available as a source of spermatozoa for artificial insemination and in vitro embryo production in many species.
📊
Key Findings & Data:The testes and epididymides were collected from 10 Anatolian buffalo bulls (about 3-4 years of age) in Samsun Province.
💡
Conclusion & Impact:There was no significant difference between fresh and frozen-thawed sperm samples for progressive sperm motility and head-acrosome anomalies of spermatozoa.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Epididymal sperm may be available as a source of spermatozoa for artificial insemination and in vitro embryo production in many species. The aim of the study was to investigate epididymal sperm freezing on spermatological parameters of Anatolian buffaloes and in vitro evaluation of frozen epididymal sperm for assisted reproductive biotechnologies. The testes and epididymides were collected from 10 Anatolian buffalo bulls (about 3-4 years of age) in Samsun Province. The testes and epididymides from each bull were removed immediately after slaughtering and transported to the laboratory on ice (+4 °C) within 40 min for further processing. After morphological measurements of testes and epididymides were determined, the spermatozoa were collected from cauda epididymides. The percentage of sperm progressive motility and abnormal spermatozoa of fresh and post-thaw sperm samples were evaluated. There was no significant difference between fresh and frozen-thawed sperm samples for progressive sperm motility and head-acrosome anomalies of spermatozoa. The middle part (P

Özet

Epididimal sperma birçok türde suni tohumlama ve in vitro embriyo üretiminde spermatozoa kaynağı olarak kullanılabilir. Yapılan bu çalışmanın amacı Anadolu Mandaları'ndan alınan epididimal spermanın dondurulmasının bazı spermatolojik parametreler üzerine etkilerinin incelenmesi ve yardımcı üreme teknolojileri için dondurulan epididimal spermanın kullanılabilirliğinin in vitro değerlendirilmesidir. Samsun İlinde kesilen (yaklaşık 3-4 yaşlı) toplam 10 adet erkek Anadolu Mandasından kesim sonrasında testisler ve epididimisler hemen alınarak buz içinde (+4 °C) 40 dakika içerisinde daha sonraki işlemler için laboratuara getirildi. Testis ve epididimislerin morfolojik ölçüleri belirlendikten sonra spermatozoa cauda epididimisten toplandı. Nativ ve çözüm sonu örneklerde progresif spermatozoa motilitesi ve anormal spermatozoa yüzde oranları değerlendirildi. Nativ ve dondurulmuş çözülmüş sperma örnekleri arasında progresif spermatozoa motilitesi ve spermatozoa baş-akrozom anomalileri yönünden herhangi bir fark tespit edilmedi. Dondurulmuş çözülmüş spermadaki orta kısım (P

🔖

Cite This Article

AKAL, E., SELCUK, M. (2022). Anadolu mandalarında testiküler morfoloji ve dondurulmuş epididimal spermanın in vitro değerlendirilmesi. Ankara Üniversitesi Veteriner Fakültesi Dergisi, Cilt: 62 - Sayı: 1, 51–55. https://ajindex.com/articles/444773

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...

References (10)

  1. Akhter S, Ansari MS, Rakha BA, Andrabi SMH, Iqbal S, Ullah N (2010): Cryopreservation of buffalo (Bubalus Bubalis) semen in Bioxcell® extender. Theriogenology, 74: 951-955.
  2. Andrabi SMH (2009): Factors affecting the quality of cryopreserved buffalo (Bubalus Bubalis) bull spermatozoa. Reprod Domest Anim, 44: 552-569.
  3. Andrabi SMH, Ansari MS, Ullah N, Anwar M, Mehmood A, Akhter S (2008): Duck egg yolk in extender improves the freezability of buffalo bull spermatozoa. Anim Reprod Sci, 104: 427-433.
  4. Annett H (2012): Artificial Insemination in Veterinary Science. In: A Bird's-Eye View of Veterinary Medicine (Carlos C. Perez-Marin ed). Available from: http://www.intechopen.com/books/a-bird-s-eye-view-of- veterinary-medicine/artificial-insemination-in- veterinaryscience. Arrighi S, Bosi G, Groppetti D, Cremonesi F (2010): Morpho- and histometric evaluations on the testis and epididymis in buffalo bulls during the different reproductive seasons. The Open Anatomy Journal, 2: 29- 33. Barati F, Khaksary MM, Mohammadi GH (2009): Cryopreservation of in situ cool stored buffalo (Bubalus Bubalis) epididymal sperm. Iranian Journal of Veterinary Research, 10: 339-345.
  5. Bearden HJ, Fuquay JW (1997): Applied animal reproduction. 4th Ed, Prentice Hall, Upper Saddle, New Jersey, 133-177.
  6. Bruemmer JE (2006): Collection and freezing of epididymal stallion sperm. Vet Clin N Am-Equine, 22: 677-682. FAO: Food and Agricultural Organizaion of the United Nations http://faostat.fao.org/site/573/DesktopDefault. aspx?PageID=573#ancor Date of Access: 21.01.2012
  7. Feradis AH, Pawitri D, Suatha IK, Amin MR, Yusuf TL, Sajuthi D, Budiarsa IN, Hayes ES (2001): Cryopreservation of epididymal spermatozoa collected by needle biopsy from cynomolgus monkeys (Macaca fascicularis). J Med Primatol, 30: 100-106.
  8. Goovaerts IGF, Hoflack GG, Van Soom A, Dewulf J, Nichi M, Kruif A, Bols PEJ (2006): Evaluation of epididymal semen quality using the Hamilton-Thorne Analyser indicates variation between the two caudae epididymides of the same bull. Theriogenology, 66: 323- 330. Graham JK (1994): Effect of seminal plasma on the motility of epididymal and ejaculated spermatozoa of the ram and bull during cryopreservation process. Theriogenelogy, 46: 1151-1162.
  9. Herold FC, Aurich JE, Gerber D (2004a): Epididymal sperm from the African Buffalo (Syncerus Caffer) can be frozen successfully with Andromed®and with TriladylTM but the addition of bovine seminal plasma is detrimental. Theriogenology, 61: 715-724.
  10. Herold FC, De