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Journals / Ankara Üniversitesi Veteriner Fakültesi Dergisi / 2015 / Cilt: 62 - Sayı: 1

Lewis ırkı rat sperması üzerine değişik sulandırıcı ve dondurma hızının etkisi

Influence of extenders and cooling rates on epididymal sperm of Lewis rat strain

Ömer VARIŞLI
Cansu AGCA
Yüksel AGCA
Pages
57–62
Publication Date
2022-10-12
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Type of extenders had significant effect on cryosurvival (p

📌 3 Key Takeaways

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Objective & Hypothesis:The objective of this study was to determine the appropriate cooling rates and extender for improving the freezability of epididymal sperm of Lewis rat strain.
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Key Findings & Data:The epididymal sperm from Lewis rats were suspended in different freezing extenders which were HEPES buffered Tyrode's lactate (TL-HEPES), modified Kreb's Ringer bicarbonate (mKRB), 3% dehydrated skim milk (SM), Salamon's Tris-citrate (TRIS), and TES/Tris (TES).
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Conclusion & Impact:Type of extenders had significant effect on cryosurvival (p
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

The objective of this study was to determine the appropriate cooling rates and extender for improving the freezability of epididymal sperm of Lewis rat strain. The epididymal sperm from Lewis rats were suspended in different freezing extenders which were HEPES buffered Tyrode's lactate (TL-HEPES), modified Kreb's Ringer bicarbonate (mKRB), 3% dehydrated skim milk (SM), Salamon's Tris-citrate (TRIS), and TES/Tris (TES). The extenders contained 20% (v/v) egg yolk (EY), 0.75% equex paste (EP) and 0.1 M raffinose or 0.1 M sucrose. The sperm samples were frozen by automated freezer, using various cooling rates (10, 40, 70, and 100 °C/min). After thawing, sperm samples were examined for integrity of plasma and acrosomal integrity, and mitochondrial membrane potential (MMP). Type of extenders had significant effect on cryosurvival (p

Özet

Çalışmanın amacı Lewis ırkı rat sperması için, uygun dondurma hızı ve sulandırıcılar tespit ederek spermanın dondurulabilirliğinin artırılmasıdır. Ratlardan alınan epididimal sperma HEPES buffered Tyrode's lactate (TL-HEPES), modified Kreb's Ringer bicarbonate (mKRB), %3 süt tozu (SM), Tris-citrate (TRIS) ve Tris/TES (TES) solüsyonları ile sulandırıldı. Sulandırıcılara 20% (v/v) yumurta sarısı, 0.75% Equex Paste ve 0.1 M rafinoz yada 0.1 M sükroz ilave edildi. Sperma örnekleri kontrollü dondurucu kullanılarak değişik soğutma hızında (10, 40, 70 ve 100 °C/dk) donduruldu. Dondurma sonrası spermalar plazma, akrozom ve mitokondri membran bütünlüğü yönünden değerlendirildi. Sulandırıcı tipinin dondurmada etkili olduğu saptandı (p

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Cite This Article

VARIŞLI, Ö., AGCA, C., AGCA, Y. (2022). Lewis ırkı rat sperması üzerine değişik sulandırıcı ve dondurma hızının etkisi. Ankara Üniversitesi Veteriner Fakültesi Dergisi, Cilt: 62 - Sayı: 1, 57–62. https://ajindex.com/articles/444762

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