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Journals / Ankara Üniversitesi Veteriner Fakültesi Dergisi / 2015 / Cilt: 62 - Sayı: 2

Perakende A sınıfı yumurtalarda Salmonella varlığının ISO 6579 metodu ve LightCycler polimeraz zincir reaksiyonu ile belirlenmesi

Presence of Salmonella in retail grade a eggs determined by the International Organization for Standardization 6579 method and a LightCycler polymerase chain reaction system

Serpil KAHYA
Seran TEMELLİ
Aysegul EYIGOR
Kamil Tayfun CARLI
Zafer ATA
Pages
125–132
Publication Date
2022-10-12
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Thesefindings indicate considerably high Salmonella contamination in retail grade A eggs.

📌 3 Key Takeaways

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Objective & Hypothesis:This study aims to determine the presence of Salmonella in naturally contaminated grade A eggs by the standardculture method International Organization for Standardization Method 6579 (ISO) and a specific real-time PCR system (LightCyclerPCR-LCPCR) to complement ISO.
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Key Findings & Data:A total of 1635 eggs pooled into 101 samples were randomly collected within one year periodfrom 20 different retail markets in Bursa, Turkey, carrying eggs of 16 large egg producers/suppliers of 5 cities with intensive layerproduction.
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Conclusion & Impact:Thesefindings indicate considerably high Salmonella contamination in retail grade A eggs.
🔬Design:Lab & Field Experiment
📍Scope:2003 · 101 samples
⚙️Variables:Domain Parameters

Abstract

This study aims to determine the presence of Salmonella in naturally contaminated grade A eggs by the standardculture method International Organization for Standardization Method 6579 (ISO) and a specific real-time PCR system (LightCyclerPCR-LCPCR) to complement ISO. A total of 1635 eggs pooled into 101 samples were randomly collected within one year periodfrom 20 different retail markets in Bursa, Turkey, carrying eggs of 16 large egg producers/suppliers of 5 cities with intensive layerproduction. Preparation of the egg and shell for analyses, Salmonella isolations and identifications, and detections were performedaccording to ISO 6887 -4:2003, ISO 6579 and LCPCR, respectively. O verall Salmonella detection rate by ISO and LCPCR were 15.8% (16/101) and 46.5 % (47/101), respectively. Out of 101 inner parts, Salmonella was detected in 11 (10.9 %) samples by ISO, andin 31 (30.7 %) samples by LCPCR. Six of 101 shell samples (5.9 %) were found to harbor Salmonella by ISO, while 18 (17.8 %)shells were positive by LCPCR. All isolates were determined as Salmonella enterica subsp. enterica serovar Enteritidis. Thesefindings indicate considerably high Salmonella contamination in retail grade A eggs. This should be under routin e monitoring byrapid methods such as PCR, complemented by standard culture to evaluate and assess the significance of risk for public health .

Özet

Bu çalışmada doğal kontamine A sınıfı yumurtalarda Salmonella varlığının ISO 6579 standart kültür metodu ve bumetodun spesifik gerçek zamanlı bir PCR sistemi (LightCycler PCR-LCPCR) ile desteklenmesini belirlemek amaçlanmıştır.Bursada 20 farklı perakende marketten 1 yıl süresince, yoğun yumurta üretimi olan 3 bölge (Ege, Marmara, Orta Anadolu)ninbüyük üretici/tedarikçisi olan 16 farklı firmaya ait 101 birleştirilmiş, toplam 1635 adet yumurta rastgele olarak örneklendi. Yumurtaların analize hazırlanması, Salmonella izolasyonu ile identifikasyonu ve deteksiyonu sırasıyla ISO 6887 -4:2003, ISO 6579agöre ve LCPCR ile yapıldı. Yumurtalarda genel Salmonella deteksiyon oranları ISO ve LCPCR ile sırası ile %15.8 (16/101) ve %46.5 (47/101) olarak belirlendi. Yüzbir iç örneğinin ISO ile 11inde (% 10.9), LCPCR ile 31inde (% 30.7) Salmonella saptandı.Kabuk örneklerinden 6sında (% 5.9) ISO ile Salmonella bulunurken, 18i (17.8 %) LCPCR ile pozitif olarak tespit edildi. Tümizolatların Salmonella enterica subsp. enterica serovar Enteritidis olduğu belirlendi. Bu bulgular perakende A sınıfı yumurtalardaSalmonella kontaminasyonunun oldukça yüksek olduğunu ve bu durumun halk sağlığı açısından önemini belirlemek vedeğerlendirebilmek için standart kültür metodu ile desteklenen PCR gibi hızlı bir metotla rutin olarak izlenmesi gerektiğinigöstermektedir.

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Cite This Article

KAHYA, S., TEMELLİ, S., EYIGOR, A., CARLI, K. T., ATA, Z. (2022). Perakende A sınıfı yumurtalarda Salmonella varlığının ISO 6579 metodu ve LightCycler polimeraz zincir reaksiyonu ile belirlenmesi. Ankara Üniversitesi Veteriner Fakültesi Dergisi, Cilt: 62 - Sayı: 2, 125–132. https://ajindex.com/articles/444737

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