SavedAdd your journal

Journals / Ankara Üniversitesi Veteriner Fakültesi Dergisi / 2016 / Cilt: 63 - Sayı: 2

Evidence of Leishmania spp. antibodies and DNA in dogs in the Middle Black Sea Region of Turkey

Murat HÖKELEK
Sinasi UMUR
Didem PEKMEZCİ
Gokmen Zafer PEKMEZCI
Cenk Soner BÖLÜKBAŞ
Ali TÜMAY GÜRLER
Pages
111–114
Publication Date
2022-10-12
DOI
—
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Our results showed that CanL could have public health implications and future screening studies are needed in this region of Turkey.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:The World Health Organization considers leishmaniasis to be one of the most important zoonotic diseases with approximately 350 million people in 98 countries at risk of contracting the disease.
📊
Key Findings & Data:For the diagnosis of the infection parasitological (direct microscopic examination of lymph nodes), serological (ELISA) and molecular (kDNA-PCR) methods were performed using blood samples obtained from 240 dogs.
💡
Conclusion & Impact:Our results showed that CanL could have public health implications and future screening studies are needed in this region of Turkey.
🔬Design:Lab & Field Experiment
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

The World Health Organization considers leishmaniasis to be one of the most important zoonotic diseases with approximately 350 million people in 98 countries at risk of contracting the disease. The leishmaniasis also remains a severe public health problem in Turkey. It is important to evaluate the prevalence of Canine leishmaniasis (CanL) for designing control policies. In the present study, we report for the first time the occurrence of Leishmania infection in dogs living in the Middle Black Sea Region of Turkey. For the diagnosis of the infection parasitological (direct microscopic examination of lymph nodes), serological (ELISA) and molecular (kDNA-PCR) methods were performed using blood samples obtained from 240 dogs. Sand flies were also collected using CDC miniature light traps from the same locations for testing Leishmania presence by kDNA-PCR. Only one (0.41%) dog out of 240 was found to be positive by both tests. Amastigotes of Leishmania spp. were detected in the same animal lymph node aspirate sample. A total of 18 sand flies were collected and no Leishmania was detected Phlebotomus spp. sample pool. Our results showed that CanL could have public health implications and future screening studies are needed in this region of Turkey.

🔖

Cite This Article

HÖKELEK, M., UMUR, S., PEKMEZCİ, D., PEKMEZCI, G. Z., BÖLÜKBAŞ, C. S., GÜRLER, A. T. (2022). Evidence of Leishmania spp. antibodies and DNA in dogs in the Middle Black Sea Region of Turkey. Ankara Üniversitesi Veteriner Fakültesi Dergisi, Cilt: 63 - Sayı: 2, 111–114. https://ajindex.com/articles/444722

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...

References (15)

  1. Aslantaş Ö, Özdemir V, Kılıç S, et al (2005): Seroepidemiology of leptospirosis, toxoplasmosis, and leishmaniosis among dogs in Ankara, Turkey. Vet Parasitol, 129, 187-191
  2. Atasoy A, Paşa S, Özensoy Toz S, et al (2010): Seroprevalence of canine visceral leishmaniasis around the Aegean cost of Turkey. Kafkas Univ Vet Fak Derg, 16, 1-6
  3. Baneth G, Koutinas AF, Solano-Gallego L, et al (2008): Canine leishmaniosis - new concepts and insights on an expanding zoonosis: part one. Trends Parasitol, 24, 324-330
  4. Ertabaklar H, Özensoy Toz S, Taylan Özkan A, et al (2005): Serological and entomological survey in a zoonotic visceral leishmaniasis focus of North Central Anatolia, Turkey: Corum province. Acta Trop, 93, 239-246
  5. Gomes AHS, Ferreira IMR, Lima MLSR, et al (2007): PCR identification of Leishmania in diagnosis and control of canine leishmaniasis. Vet Parasitol, 144, 234-241
  6. Miró G, Cardoso L, Pennisi MG, et al (2008): Canine leishmaniosis - new concepts and insights on an expanding zoonosis: part two. Trends Parasitol, 24, 371-377
  7. Motazedian H, Karamian M, Noyes HA, et al (2002): DNA extraction and amplification of Leishmania from archived, Giemsa-stained slides, for the diagnosis of cutaneous leishmaniasis by PCR. Ann Trop Med Parasit, 96, 31-34
  8. Ok ÜZ, Balcıoğlu İC, Özkan AT, et al (2002): Leishmaniasis in Turkey. Acta Tropica, 84, 43-48
  9. Özbel Y (2013): The infections transmitted by Sand flies in Turkey. Ankara Univ Vet Fak Derg, 60, 225-228
  10. Özbel Y, Oskam L, Özensoy S, et al (2000): A survey on canine leishmaniasis in western Turkey by parasite, DNA and antibody detection assays. Acta Tropica, 74, 1-6
  11. Özensoy Toz S, Ertabaklar H, Özbel Y, et al (2005): Seroprevalance of canine visceral leishmaniasis in Kusadasi, Turkey. Turkish J Vet Anim Sci, 29, 23-26
  12. Özensoy Toz S, Özbel Y, Ertabaklar H, et al (2005): Comparisons of clinical findings and serological data in the diagnosis of canine leishmaniosis. Turk J Vet Anim Sci, 29, 269-273
  13. Özensoy Toz S, Özbel Y, Turgay N, et al (1998): Serodiagnosis and epidemiology of visceral leishmaniasis in Turkey. Am J Trop Med Hyg, 59, 363-369
  14. Özensoy Toz S, Şakru N, Ertabaklar H, et al (2009): Serological and entomological survey of zoonotic visceral leishmaniasis in Denizli province, Aegean Region, Turkey. New Microbiologica, 32, 93-100
  15. Özerdem D, Eroğlu F, Genç A, et al (2009): Comparison of microscopic examination, rK39, and PCR for visceral leishmaniasis diagnos