SavedAdd your journal

Journals / Ankara Üniversitesi Veteriner Fakültesi Dergisi / 2016 / Cilt: 63 - Sayı: 3

Koyun beta-laktoglobulin geni ekzon 2 bölgesine ait genetik polimorfizmin araştırılması ve bazı süt verim özellikleri ile ilişkisi

Investigating the genetic polymorphism in the exon 2 region of ovine beta-lactoglobulin gene and its association with some milk traits

Özge ÖZMEN
Selim KUL
Pages
323–328
Publication Date
2022-10-12
DOI
—
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

In this research, ?-LGB showed a significant effect on milk yield in the Sakiz ewes, but further studies are necessary to confirm the association reported here.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:?-lactoglobulin (?-LGB) gene is the major whey protein of ruminant and is synthesized by secreting cells of mammary gland during pregnancy and lactation and its biological function is still unclear.
📊
Key Findings & Data:Therefore, the purpose of the study was to identify genotype frequencies of single nucleotide polymorphisms (SNPs) in ovine ?-LGB gene exon 2 region and its possible association with milk traits in dairy sheep breeds.
💡
Conclusion & Impact:In this research, ?-LGB showed a significant effect on milk yield in the Sakiz ewes, but further studies are necessary to confirm the association reported here.
🔬Design:Research Article
📍Scope:450 samples
⚙️Variables:Domain Parameters

Abstract

?-lactoglobulin (?-LGB) gene is the major whey protein of ruminant and is synthesized by secreting cells of mammary gland during pregnancy and lactation and its biological function is still unclear. Therefore, the purpose of the study was to identify genotype frequencies of single nucleotide polymorphisms (SNPs) in ovine ?-LGB gene exon 2 region and its possible association with milk traits in dairy sheep breeds. The genetic structures of the SNPs were examined by PCR-RFLP and DNA sequencing in three sheep populations. A hundred fifty blood and milk samples each from Sakiz, Akkaraman and Awassi ewes, with total of 450 samples were used in the experiment. Awassi sheep breed was found as monomorphic for ?-LGB gene in exon 2 region. Genotype AB showed a strong association with milk yields content (P A SNPs of the ?-LGB gene for exon 2. In this research, ?-LGB showed a significant effect on milk yield in the Sakiz ewes, but further studies are necessary to confirm the association reported here.

Özet

?-laktoglobulin (?-LGB) geni ruminantlarda en temel peynir altı suyu protenidir; gebelik ve laktasyon süresince meme bezi hücrelerinden salgılanmaktadır; biyolojik fonksiyonu ise henüz tam olarak bilinmemektedir. Dolayısıyla, bu çalışmanın amacı koyun ?-LGB geni ekzon 2 bölgesine ait tek nükleotit polimorfizmlerinin (TNP) genotip frekanslarının saptanması ve bu olası TNP'lerin süt verim özellikleri ile ilişkisinin belirlenmesidir. Çalışmada üç koyun popülasyonunda belirlenen TNP'lerin genetik yapısı PCR-RFLP ve DNA dizi analizi metodu ile incelenmiştir. Araştırmada toplam 450 örnek olmak üzere Sakız, Akkaraman ve İvesi ırkı koyunlardan 150'şer adet kan ve süt örneği kullanılmıştır. İvesi koyun ırkı ?-LGB geni ekzon 2 bölgesi için monomorfik bulunmuştur. Sakız koyun ırkında AB genotipi ile süt verimi arasında önemli bir ilişki bulunurken (P A SNP ilk kez tanımlanmıştır. Bu araştırmada, Sakız koyun ırkında ?-LGB geninin süt verimi üzerine önemli bir etkisi olduğu belirlenmiştir, ancak elde edilen bu bulguların bu konuda yapılacak ilişkilendirme çalışmaları ile doğrulanması gerekmektedir.

🔖

Cite This Article

ÖZMEN, Ö., KUL, S. (2022). Koyun beta-laktoglobulin geni ekzon 2 bölgesine ait genetik polimorfizmin araştırılması ve bazı süt verim özellikleri ile ilişkisi. Ankara Üniversitesi Veteriner Fakültesi Dergisi, Cilt: 63 - Sayı: 3, 323–328. https://ajindex.com/articles/444717

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...

References (14)

  1. Akcapinar H (2000): Koyun Yetiştiriciliği, Ismat Press, Ankara, pp 20-25
  2. Amigo L, Recio I, Ramos M (2000): Genetic polymorphism of ovine milk proteins: its influence on technological properties of milk--a review. Int Dairy J, 10,135-149
  3. Arora R, Bhatia S, Mishra BP, et al. (2010): Genetic polymorphism of the ?-lactoglobulin gene in native sheep from India. Biochem Genet, 48, 304-311
  4. Barillet F (2007): Genetic improvement for dairy production in sheep and goats. Small Rumin Res, 70, 60- 75
  5. Barillet F, Arranz JJ, Carta A (2005): Mapping quantitative trait loci for milk production and genetic polymorphisms of milk proteins in dairy sheep. Genet Sel Evol, 37,109-123
  6. Carta A, Casu S, Salaris S (2009): Invited review: Current state of genetic improvement in dairy sheep. J Dairy Sci, 92,5814-5833
  7. Celik S, Ozdemir S (2006): ?-Lactoglobulin variants in awassi and Morkaraman Sheep and their association with the composition and rennet clotting time of the milk. Turk J Vet Anim Sci, 30, 539-544
  8. Chessa S, Bolla P, Dario C, et al. (2003): Polimorfismi genetici lattoproteici nella razza ovina gentile di puglia: Monitoraggio mediante focalizzazione isoelectrica. Sci Tecn Lat Cas, 54,1-8
  9. Corral JM, Padilla JA, Izquierdo M, et al. (2013): Detection and genetic characterization of ovine CSN1S2B polymorphisms and their associations with milk production traits. Livest Sci, 153,10-19
  10. Di Stasio L, Portolano B, Todaro M, et al. (1997): Effect of ovine ?-lactoglobulin phenotype on cheese yield and composition, in Proc. IDF Seminar Milk Protein Polymorphism. IDF Publishers, Brussels. pp. 324-327
  11. Elmaci C, Oner Y, Balcioglu SM (2006): Genetic polymorphism of ?-lactoglobulin gene in native Turkish sheep breeds. Biochem Genet, 44, 7-8
  12. Erhardt G (1989): Genetic polymorphisms of ?- lactalbumin and ?-lactoglobulin in sheep milk. Anim Genet, 20,76-77
  13. Excoffier L, Laval G, Schneider S (2006): Arlequin Version 3.01. An Integrated Software Package for Population Genetics Data Analysis. Computational and Molecular Population Genetics Lab (CMPG), Inst. of Zoology Univ. of Berne. http://cmpg.unibe.ch/software/arlequin3/, Accessed 17 March 2014 14. FAO (Food and Agriculture Organization of The United Nations), Statistical Databases (http://faostat3.fao.org/home/E), Accessed 25 December 2014
  14. Feligini M, Parma P, Aleandri R, et al. (1998): PCRRFLP test for direct determination of ?-lactoglobulin genotype in sheep. Anim Genet, 29, 473-474.