Journals / Ankara Üniversitesi Veteriner Fakültesi Dergisi / 2016 / Cilt: 63 - Sayı: 4

Staphylococcus aureus izolatlarının mecA ve mecC genleri taşıyıcılığı açısından taranması

Screening of Staphylococcus aureus isolates for mecA and mecC genes carriage

Pages
389–391
Publication Date
2022-10-12
DOI
—
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Inhibition zone diameters for cefoxitin

📌 3 Key Takeaways

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Objective & Hypothesis:Methicillin-resistant Staphylococcus aureus (MRSA) is an important pathogen which causes hospital infections and different systemic infections.
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Key Findings & Data:MecC gene, is a mecA gene homologue showing ~ 69 DNA similarities to the mecA gene. mecC gene positive S. aureus was firstly detected in livestock.
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Conclusion & Impact:Inhibition zone diameters for cefoxitin
🔬Design:Research Article
📍Scope:2011 Verileri
⚙️Variables:Domain Parameters

Abstract

Methicillin-resistant Staphylococcus aureus (MRSA) is an important pathogen which causes hospital infections and different systemic infections. MRSA is clarified by the acquisition of the mecA gene, which is located on the staphylococcal cassette chromosome mec (SCCmec). MecC gene, is a mecA gene homologue showing ~ 69 DNA similarities to the mecA gene. mecC gene positive S. aureus was firstly detected in livestock. Because of that, livestock constitute a reservoir of S. aureus harboring mecC gene in terms of spread to human. The aim of this study was to determine the mecC gene in 177 S. aureus isolates collected from two distinct veterinary laboratories (Burdur and Aydin) between 2011 and 2013. Microorganisms were firstly defined by colony morphology, Gram stain, catalase test, mannitol fermentation and coagulase tests. After that, further identification process was performed by MALDI-TOF MS (Bruker Daltonics, Billerica, MA, USA). Antibiotic susceptibility testing by Kirby-Bauer disk diffusion method with oxacillin (1 ?g) and cefoxitin (30 ?g) was performed according to the CLSI standards. Inhibition zone diameters for cefoxitin

Özet

Metisilin-dirençli Staphylococcus aureus (MRSA) hastane enfeksiyonları ve farklı sistemik enfeksiyonlara yol açan önemli bir patojendir. MRSA oluşumu stafilokok kaset kromozom mec (SCCmec) üzerinde yer alan mecA geni edinimi ile oluşur. MecC geni, mecA genine ~69 DNA benzerlik gösteren bir mecA geni homoloğudur. MecC geni pozitif S. aureus ilk olarak çiftlik hayvanlarında tespit edilmiştir. Bu nedenle çiftlik hayvanları mecC geni barındıran S. aureus'un insanlara yayılması açısından rezervuar konumunda bulunmaktadır. Bu çalışmanın amacı, 2011 ve 2013 yılları arasında iki ayrı veteriner laboratuvarından (Burdur ve Aydın) toplanmış olan 177 S. aureus izolatında mecC geni varlığını saptamaktır. Mikroorganizmalar öncelikle koloni morfolojisi, Gram boyama, katalaz testi, mannitol fermantasyonu ve koagülaz testleri ile tanımlandı. Daha sonra ileri tanımlama işlemi MALDI-TOF MS (Bruker Daltonics, Billerica, MA, ABD) ile yapıldı. Antibiyotik duyarlılık testi oksasilin (1 ?g) ve sefoksitin (30 ?g) için Kirby-Bauer disk difüzyon yöntemi ile CLSI standartlarına göre yapıldı. İnhibisyon zon çapı sefoksitin için

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Cite This Article

ÖZTÜRK, D., TÜRKYILMAZ, S., DOĞAN, E., KILIÇ, A., TÜRÜTOĞLU, H. (2022). Staphylococcus aureus izolatlarının mecA ve mecC genleri taşıyıcılığı açısından taranması. Ankara Üniversitesi Veteriner Fakültesi Dergisi, Cilt: 63 - Sayı: 4, 389–391. https://ajindex.com/articles/444713

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