Journals / Ankara Üniversitesi Veteriner Fakültesi Dergisi / 2016 / Cilt: 63 - Sayı: 3

Characterization of mesenchymal stem cells isolated from the adipose tissue of young and old dogs

Genç ve yaşlı köpeklerin yağ dokusundan elde edilen mezenkimal kök hücrelerin karakterizasyonu

Pages
297–302
Publication Date
2022-10-12
DOI
—
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Furthermore, a comparison was made for the stem cell potential of the lipoaspirates.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:The use of mesenchymal cells in veterinary medicine is considered to be promising for the treatment of several diseases.
📊
Key Findings & Data:Previous research has demonstrated success in the transplantation of canine adipose mesenchymal cells.
💡
Conclusion & Impact:Furthermore, a comparison was made for the stem cell potential of the lipoaspirates.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

The use of mesenchymal cells in veterinary medicine is considered to be promising for the treatment of several diseases. Due to adipose mesenchymal stem cells being readily available and the expansion of these cells not incurring any additional cost, the culturing of adipose mesenchymal cells has become a commonly applied method. Adipose mesenchymal cells are known to have a multilineage potential of differentiation. Previous research has demonstrated success in the transplantation of canine adipose mesenchymal cells. This study was aimed at the comparative evaluation of mesenchymal stem cells isolated from adipose tissue samples taken at surgery from 3 old dogs (above 5 years of age) and 3 young dogs (1 to 6-months-old or younger), and at the determination of any differences in the adipogenic, chondrogenic, and osteogenic differentiation potential, differentiation rate, proliferation rate, and characteristics of these cells. Furthermore, a comparison was made for the stem cell potential of the lipoaspirates.

Özet

Mezenkimal kök hücrelerin veteriner hekimlik alanında kullanımı birçok hastalığın tedavisi için umut ışığı olarak görülmektedir. Yağ doku kökenli mezenkimal kök hücrelerin eldesinin kolay olması ve üretiminin ek bir maliyet getirmemesi sebebiyle yağ dokudan kök hücre üretimi en çok kullanılan yöntem olarak karşımıza çıkmaktadır. Yağ doku kökenli hücrelerin multilineage potansiyeline sahip olduğu bilinmektedir. Köpek yağ doku kaynaklı mezenkimal kök hücrelerin başarılı bir şekilde transplante edildiği de daha önceki çalışmalarda da bildirilmiştir. Bu çalışmada 3 adet yaşlı (5 yaş üzeri) ve 3 adet genç (6 ay ve altı) köpekten operasyon sırasında alınan yağ doku örneklerinden elde edilen mezenkimal kök hücrelerin karşılaştırılması, adipojenik, kondrojenik ve osteojenik farklılaşma potansiyeli, farklılaşma hızı, üreme hızı ve karakteristik özellikleri yönünden aralarındaki farkların ortaya konulması, lipoaspiratın içerdiği kök hücre potansiyelinin karşılaştırılması amaçlanmıştır.

🔖

Cite This Article

BAYRAKTAROĞLU, A. G., ÖZEN, A., CAN, P., CEYLAN, A., GÜL, İ. S. (2022). Characterization of mesenchymal stem cells isolated from the adipose tissue of young and old dogs. Ankara Üniversitesi Veteriner Fakültesi Dergisi, Cilt: 63 - Sayı: 3, 297–302. https://ajindex.com/articles/444667

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...

References (15)

  1. Can A (2014): Kök hücre. Akademisyen Tıp Kitabevi, Yenişehir, Ankara
  2. Csaki C, Matis U, Mobasheri A, et al. (2007): Chondrogenesis, osteogenesis and adipogenesis of canine mesenchymal stem cells: a biochemical, morphological and ultrastructural study. Histochem Cell Biol, 128, 507- 20
  3. Diaz-Romero J, Quintin A, Schoenholzer E, et al. (2013): S100A1 and S100B Expression Patterns Identify Differentiation Status of Human Articular Chondrocytes. J Cell Physiol, 229, 1106-1117
  4. Gartner LP, Hiatt JL (1997): Colour textbook of histology. WB Sounders Company, Philadelphia, Pensylvania
  5. Gül Sancak İ, Özen A, Alparslan Pınarlı F, et al. (2014): Limbal Stem Cells in dogs and cats their identification culture and differentiation into keratinocytes. Kafkas Univ Vet Fak Derg, 20, 909-914
  6. Halvorsen YD, Bond A, Sen A, et al. (2001): Thiazolidinediones and glucocorticoids synergistically induce differentiation of human adipose tissue stromal cells: Biochemical, cellular, and molecular analysis. Metabolism, 50, 407-413
  7. Awad HA, Butler DL, Boivin GP, et al. (1999): Autologous Mesenchymal Stem Cell-Mediated Repair of Tendon. Tissue Engineering, 5, 267-277
  8. Justesen J, Pedersen SB, Stenderup K, et al. (2004): Subcutaneous adipocytes can differentiate into boneforming cells in vitro and in vivo. Tissue Eng, 10, 381-391
  9. Karaöz E, Aksoy A, Ayhan S, et al. (2009): Characterization of mesenchymal stem cells from rat bone marrow: ultrastructural properties, differentiation potential and immunophenotypic markers. Histochem Cell Biol, 132, 533-546
  10. Lettry V, Hosoya K, Takagi S, et al. (2010): Coculture of equine mesenchymal stem cells and mature equine articular chondrocytes results in improved chondrogenic differentiation of the stem cells. Jpn J Vet Res, 58, 5-15
  11. Meliga E, Strem BM, Duckers HJ, et al. (2007): Adipose-derived cells. Cell Trans, 16, 963-970
  12. Miranville A, Heeschen C, Sengenes C, et al. (2002): Improvement of postnatal neovascularization by human adipose tissue-derived stem cells. Circulation, 110, 349- 355
  13. Mizuno H, Zuk PA, Zhu M, et al. (2002): Myogenic differentiation by human processed lipoaspirate cells. Plast Reconstr Surg, 109, 199-209
  14. Orbay H, Tobita M, Mizuno H (2012): Mesenchymal Stem Cells Isolated from Adipose and Other Tissues: Basic Biological Properties and Clinical Applications. Stem Cells International Volume, Article ID 461718, 9.
  15. Ozen A, Gul Sancak I, Koch