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Journals / Ankara Üniversitesi Veteriner Fakültesi Dergisi / 2017 / Cilt: 64 - Sayı: 3

Yaşlı farelerde testosteron takviyesinin aorta thoracica'nın morfometri ve VEGF ekspresyon seviyesi üzerine etkisi

The effect of testosterone supplementation on morphometry and VEGF expression level of thoracic aorta in aged mice

İlknur DABANOĞLU
Mehmet Utkan ÖREN
Pages
149–154
Publication Date
2022-10-12
DOI
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⚡ TL;DR(Too Long; Didn't Read)

Although the alterations in the level of testosterone had no significant effects on intima or media thickness of the male thoracic aorta, in female, there was a significant increase (p

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Objective & Hypothesis:The aim of this study was to determine the effects of the testosterone hormone level on morphometry and vascular endothelial growth factor (VEGF) expression level of thoracic aorta of aged mice using histological and molecular techniques.
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Key Findings & Data:A total of 30 aged mice (15 males and 15 females) were enrolled in 3 study groups: sham operation group (Control), gonadectomy group (G) and gonadectomy and testosterone supplementation group (GTS).
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Conclusion & Impact:Although the alterations in the level of testosterone had no significant effects on intima or media thickness of the male thoracic aorta, in female, there was a significant increase (p
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

The aim of this study was to determine the effects of the testosterone hormone level on morphometry and vascular endothelial growth factor (VEGF) expression level of thoracic aorta of aged mice using histological and molecular techniques. A total of 30 aged mice (15 males and 15 females) were enrolled in 3 study groups: sham operation group (Control), gonadectomy group (G) and gonadectomy and testosterone supplementation group (GTS). Serum testosterone levels were measured by ELISA. Intima and media thickness and inner diameter of thoracic aorta were measured by morphometric investigation. The levels of the angiogenic factor, VEGF (vascular endothelial growth factor) mRNA in thoracic aorta were determined by RT-PCR. Although the alterations in the level of testosterone had no significant effects on intima or media thickness of the male thoracic aorta, in female, there was a significant increase (p

Özet

Çalışmanın amacı, yaşlı farelerde testosteron hormonu seviyesinin aorta thoracica'daki anjiogenik etkisini ve vaskülo endoteliyal büyüme faktörünün (VEGF) ekspresyon düzeyini histolojik ve moleküler yöntemlerle belirlemektir. Çalışmada 30 adet yaşlı fare (15 erkek ve 15 dişi) üç farklı gruba ayrıldı. Bu gruplar; yalancı gonadektomi operasyonu yapılan kontrol grubu, gonadektomi operasyonu yapılan (G) grup ve hem gonadektomi hem de testosteron takviyesi yapılan (GTS) gruptur. Hayvanların kanındaki testosteron seviyesi ELISA testi ile ölçüldü. Aorta thoracica'nın intima ve media kalınlıkları ile iç çapı morfometrik incelemelerle belirlendi. Aorta thoracica'daki anjiogenik faktörlerden biri olan VEGF mRNA miktarı ise RT-PCR tekniği ile tespit edildi. Testosteron seviyesindeki değişimlerin erkeklerde aorta thoracica'nın intima ve media kalınlıklarında önemli bir etkisinin olmadığı saptandı. Dişilerde ise gonadektomi operasyonundan sonra yapılan hormon takviyesinin intima kalınlığında bir artışa (p

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Cite This Article

DABANOĞLU, İ., ÖREN, M. U. (2022). Yaşlı farelerde testosteron takviyesinin aorta thoracica'nın morfometri ve VEGF ekspresyon seviyesi üzerine etkisi. Ankara Üniversitesi Veteriner Fakültesi Dergisi, Cilt: 64 - Sayı: 3, 149–154. https://ajindex.com/articles/444654

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