SavedAdd your journal

Journals / Ankara Üniversitesi Veteriner Fakültesi Dergisi / 2017 / Cilt: 64 - Sayı: 3

Modifiye atmosfer paketli (MAP) sığır kıyma ve kuşbaşı et örneklerinde Clostridium difficile ve toksin genlerinin belirlenmesi

Detection of Clostridium difficile and toxin genes in samples of modified atmosphere packaged (MAP) minced and cubed beef meat

Fatma ATASOY
Ali GÜCÜKOĞLU
Pages
165–170
Publication Date
2022-10-12
DOI
—
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

The result of this study demonstrated that C. difficile strains detected in samples of modified atmosphere packaged (MAP) beef meat could be a potential problem to public health

📌 3 Key Takeaways

🎯
Objective & Hypothesis:In this study it is intended to find out the prevalence of Clostridium difficile in modified atmosphere packaged (MAP) minced (n:50) and cubed beef meat samples (n :50); determine the toxin from the isolates and detect the antibiotic susceptibility against metronidazole, vancomycin and clindamycin.
📊
Key Findings & Data:C. difficile isolates have been isolated from two (4%) of 50 minced beef meat samples; one (2%) of 50 cubed beef meat samples.
💡
Conclusion & Impact:The result of this study demonstrated that C. difficile strains detected in samples of modified atmosphere packaged (MAP) beef meat could be a potential problem to public health
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

In this study it is intended to find out the prevalence of Clostridium difficile in modified atmosphere packaged (MAP) minced (n:50) and cubed beef meat samples (n :50); determine the toxin from the isolates and detect the antibiotic susceptibility against metronidazole, vancomycin and clindamycin. C. difficile isolates have been isolated from two (4%) of 50 minced beef meat samples; one (2%) of 50 cubed beef meat samples. All three isolates were confirmed by PCR as C. difficile by the detection of tpi gene. Three out of 5 C. difficile isolates showed toxigenic character, two of them were carried type B (tcdB) toxin genes, one of them harbored type A (tcdA) toxin gene. When antibiotic resistance profile was phenotypically analyzed, only C. difficile type A (tcdA) was found resistant against clindamycin. All isolates were found sensitive to vancomycin and metronidazole. The result of this study demonstrated that C. difficile strains detected in samples of modified atmosphere packaged (MAP) beef meat could be a potential problem to public health

Özet

Bu çalışmada konvansiyonel yöntem ve multipleks PCR (mPCR) tekniği ile modifiye atmosfer paketli (MAP) sığır kıyma (n:50) ve kuşbaşı (n:50) örneklerinde Clostridium difficile prevalansı, izolatlardaki toksin gen tipinin belirlenmesi ve metronidazol, vankomisin ve klindamisin antibiyotiklerine karşı duyarlılıklarının tespiti amaçlanmıştır. 50 sığır kıyma örneğinin ikisinde (% 4), 50 sığır kuşbaşı örneğinin birinde (% 2), C. difficile saptanmıştır. Multipleks PCR ile yapılan analizlerde, konvansiyonel yöntemle belirlenen toplam beş izolat ile tpi geninin tespiti ile C. difficile olarak doğrulanmıştır. C. difficile izolatlarının toksin gen varlığı değerlendirildiğinde beş izolatın üçü toksijenik karakterde olup ikisinde C. difficile tip B (tdcB), birinde ise C. difficile tip A (tdcA) toksin geni tespit edilmiştir. Fenotipik antibiyotik direnç profili analizinde klindamisine karşı yalnızca C. difficile tip A (tdcA) toksin genini içeren izolat dirençli bulunurken, tüm izolatlar vankomisine ve metronidazole karşı duyarlı bulunmuştur. Çalışma sonucunda modifiye atmosfer paketli (MAP) et örneklerinde tespit edilen C. difficile'nin halk sağlığı için potansiyel bir tehlike olabileceği ortaya konmuştur

🔖

Cite This Article

ATASOY, F., GÜCÜKOĞLU, A. (2022). Modifiye atmosfer paketli (MAP) sığır kıyma ve kuşbaşı et örneklerinde Clostridium difficile ve toksin genlerinin belirlenmesi. Ankara Üniversitesi Veteriner Fakültesi Dergisi, Cilt: 64 - Sayı: 3, 165–170. https://ajindex.com/articles/444639

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...

References (11)

  1. Baker AA, Davis E, Rehberger T, et al. (2010): Prevalence and diversity of toxigenic Clostridium perfringens and Clostridium difficile among swine herds in the midwest. Appl Environ Microbiol, 76, 2961-2967.
  2. Bauer MP, Notermans DW, Van Benthem BH (2011): Clostridium difficile infection in Europe: A hospital-based survey. Lancet, 377, 63-73.
  3. Boer E, Zwartkruis-Nahuis A, Heuvelink AE, et al. (2011): Prevalence of Clostridium difficile in retailed meat in The Netherlands. Int J Food Microbiol, 144, 561-564.
  4. Bouttier S, Barc M, Felix B, et al. (2005):
  5. Clostridium difficile in ground meat, France. Emerg Infect Dis, 16, 733. Clinical and Laboratory Standards Institute (CLSI) (2012): Methods for Antimicrobial Susceptibility Testing of Anaerobic Bacteria; Approved Standard - Eighth Edition, M11A8E W. Hecht, et al 2012: Clinical and Laboratory Standards Institute. Curry SR, Marsh JW, Schlackman JL, et al. (2005): Prevalence of Clostridium difficile in uncooked ground meat products from Pittsburgh, Pennsylvania. Appl Envir Microbiol, 78, 4183. Deneve C, Janoir C, Poilane I, et al. (2009): New trends in Clostridium difficile virulence and pathogenesis. Int J Antimicrob Agent, 33 Suppl 1, 24-28.
  6. Esfandiari Z, Jalali M, Ezzatpanah H, et al. (2014): Prevalence and characterization of Clostridium difficile in beef and mutton meats of Isfahan region, Iran. Jundishapur J Microbiol, 7, 16771. Harvey RB, Norman KN, Andrews K, et al. (2011): Clostridium difficile in retail meat and processing plants in Texas. J Vet Diag Invest, 23, 807-811.
  7. Hoffer E, Haechler H, Frei R, et al. (2010): Low occurrence of Clostridium difficile in fecal samples of healthy calves and pPigs at slaughter and in minced meat in Switzerland. J Food Prot, 73, 973-975.
  8. Houser BA, Soehnlen MK, Wolfgang DR, et al. (2012): Prevalence of Clostridium difficile toxin genes in the feces of veal calves and incidence of ground veal contamination. Foodborne Pathog Dis, 9, 32-36.
  9. Janezic S, Ocepe, M, Zidaric M, et al. (2012): Clostridium difficile genotypes other than ribotype 078 that are prevalent among human, animal and environmental isolates. BMC Microbiol, 12, 48. Jöbstl M, Heuberger S, Indra A, et al. (2008): Clostridium difficile in raw products of animal origin. Int J Food Microbiol, 138, 172-175.
  10. Kouassi KA, Dadie AT, N'Guessan KF, et al. (2014): Clostridium perfringens and Clostridium difficile in cooked beef sold in C^ote d'Ivoire and their antimicrobial susceptibility. Anaerob, 28, 90-94.
  11. Lemee L, Dhall