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Journals / Ankara Üniversitesi Veteriner Fakültesi Dergisi / 2017 / Cilt: 64 - Sayı: 1

Genotyping of Echinococcus granulosus obtained from cattle slaughtered in Kırıkkale Province

Kırıkkale'de kesilen sığırlarda Echinococcus granulosus'un moleküler olarak genotiplendirilmesi

Sami GÖKPINAR
Rukiye DEĞİRMENCİ
Kader YILDIZ
Pages
51–54
Publication Date
2022-10-12
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

In the present study, the isolates were identified as G1-G3 complex according to COI sequencing results.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Strain differences are responsible from some important differences such as host specifity, growth rate, antigenic properties, sensitivity to chemotherapeutic agents, transmission dynamics and epidemiological variations in Echinococcus granulosus.
📊
Key Findings & Data:For this aim, mitochondrial cytochrome c oxidase subunit 1 (COI) and 12S rRNA genes of E. granulosus isolates amplified by specific primers and then COI gene sequence was made.
💡
Conclusion & Impact:In the present study, the isolates were identified as G1-G3 complex according to COI sequencing results.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Strain differences are responsible from some important differences such as host specifity, growth rate, antigenic properties, sensitivity to chemotherapeutic agents, transmission dynamics and epidemiological variations in Echinococcus granulosus. In this study, it was aimed to detection strain identification of E. granulosus obtained from liver and lungs of cattle slaughtered in Kırıkkale region. For this aim, mitochondrial cytochrome c oxidase subunit 1 (COI) and 12S rRNA genes of E. granulosus isolates amplified by specific primers and then COI gene sequence was made. In the present study, the isolates were identified as G1-G3 complex according to COI sequencing results.

Özet

Echinococcus granulosus türü içindeki suş farklılıkları etkenin konak spesifitesi, gelişim hızı, patojenitesi, antijenik özellikleri, kemoterapotiklere duyarlılığı, bulaşma dinamikleri ve epidemiyolojide farklılıklar şekillendirmektedir. Bu çalışmada; Kırıkkale Yöresi'nde kesilen sığırların karaciğer ve akciğerlerinden elde edilen E. granulosus izolatlarının suş identifikasyonu yapılması amaçlanmıştır. Bunun için parazitin 12S rRNA ve mitokondriyal cytochrome c oxidase subunit 1 (COI) geni spesifik primerlerle çoğaltılmıştır ve COI geni sekanslatılmıştır. COI sekans sonucuna göre, elde edilen izolatların G1-G3 kompleksinde olduğu belirlenmiştir.

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Cite This Article

GÖKPINAR, S., DEĞİRMENCİ, R., YILDIZ, K. (2022). Genotyping of Echinococcus granulosus obtained from cattle slaughtered in Kırıkkale Province. Ankara Üniversitesi Veteriner Fakültesi Dergisi, Cilt: 64 - Sayı: 1, 51–54. https://ajindex.com/articles/444638

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