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Journals / Ankara Üniversitesi Veteriner Fakültesi Dergisi / 2017 / Cilt: 64 - Sayı: 3

Investigation of the diagnostic value of serology, cytology and polymerase chain reaction in cat toxoplasmosis

Kedilerde toksoplazmoz tanısında seroloji, sitoloji ve polimeraz zincir reaksiyonunun tanısal değerlerinin araştırılması

Oğuz KUL
Zeynep PEKCAN
İlknur PİR YAĞCI
Sibel YASA DURU
Cahit BABÜR
Ahmet DENİZ
Pages
199–203
Publication Date
2022-10-12
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

In conclusion, although the seroprevalance was 48.03%, systemic toxoplasmosis rate was found 0.98% (1/49) with a lower percentage

📌 3 Key Takeaways

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Objective & Hypothesis:Toxoplasmosis is an infectious disease affecting most of the mammalian and aviary animals caused by Toxoplasma gondii.
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Key Findings & Data:The material of the study was constituted by blood, stool and bronchoalveoloar lavage samples taken from totally 102 cats.
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Conclusion & Impact:In conclusion, although the seroprevalance was 48.03%, systemic toxoplasmosis rate was found 0.98% (1/49) with a lower percentage
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Toxoplasmosis is an infectious disease affecting most of the mammalian and aviary animals caused by Toxoplasma gondii. In this study, diagnostic values of bronchoalveolar lavage, biopsy and PCR and their practical implementation ways were questioned. The results of the each test were evaluated. Additionally, incidence of toxoplasmosis among seropositive cats were investigated by T. gondii and oocyst shedding screened in the stool samples, as well. The material of the study was constituted by blood, stool and bronchoalveoloar lavage samples taken from totally 102 cats. Bronchoalveolar lavage samples were evaluated for cytologic examination. In PCR analysis, T. gondii B1 primary sets were used and conventional-PCR procedure was applied. Antibodies to T. gondii were found in 49 (48,03%) of 102, with titers of 1/16 in 41 (40,19%), 1/64 in 8 (7,84%) cats. Only one cat exhibited systemic toxoplasmosis. In conclusion, although the seroprevalance was 48.03%, systemic toxoplasmosis rate was found 0.98% (1/49) with a lower percentage

Özet

Toksoplazmozis, Toxoplasma gondii tarafından meydana getirilen, çok sayıda memeli ve kanatlı hayvanda görülen, protozoer bir enfeksiyondur. Bu çalışmada; klinik belirti gösteren ya da T. gondii seropozitif bulunan kedilerde uygulanabilecek bronkoalveolar lavaj ve PZR tanı yöntemlerinin geçerliliği sorgulanarak, tanısal önemleri araştırıldı. Ayrıca, seropozitif bulunan kediler arasında sistemik toksoplazmozis görülme sıklığı ile dışkıda ookist varlığı muayene edildi. Çalışma materyalini, 102 kediden alınan kan, uygun olgularda ise bronkoalveolar lavaj, deri ve karaciğer biyopsileri oluşturdu. Bu kapsamda, kan serumlarında Sabin-Feldman boya testi ile T. gondii seropozitif bulunan kediler seçilerek; anestezi altında endotrakeyal kateter yardımıyla bronkoalveolar lavaj örneğinde sitolojik muayene yapıldı. PZR analizinde ise T. gondii B1 geni spesifik primerler ile konvansiyonel-PZR prosedürleri uygulandı. Çalışmada incelenen kedilerde T. gondii seroprevalansı 49/102 (%48.03) olarak belirlendi. Anti-Toxoplasma gondii antikor titreleri açısından incelendiğinde; bunlardan %40.19'unun (41/102) 1/16, %7.84'ünün (8/102) ise 1/64 titrede olduğu ortaya konuldu. Sonuç olarak, seroprevalans %48.03 olmasına rağmen sistemik toksoplazmozis oranının (49 seropozitif kediden biri, %0.98) oldukça düşükgerçekleştiği belirlendi

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Cite This Article

KUL, O., PEKCAN, Z., YAĞCI, İ. P., DURU, S. Y., BABÜR, C., DENİZ, A. (2022). Investigation of the diagnostic value of serology, cytology and polymerase chain reaction in cat toxoplasmosis. Ankara Üniversitesi Veteriner Fakültesi Dergisi, Cilt: 64 - Sayı: 3, 199–203. https://ajindex.com/articles/444624

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