Journals / Ankara Üniversitesi Veteriner Fakültesi Dergisi / 2018 / Cilt: 65 - Sayı: 4

Allergen specific IgE determination by in vitro allergy test in head and facial feline dermatitis: A pilot study

Baş ve yüz dermatitli kedilerde in vitro alerji testi ile alerjen spesifik IgE tayini: bir pilot çalışma

Pages
379–386
Publication Date
2022-10-12
DOI
—
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Positive reactions in healthy cats might indicate a subclinical hypersensitivity state or the requirement for investigating other relevant factors.

📌 3 Key Takeaways

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Objective & Hypothesis:Pruritus is one of the most common clinical findings in cats which have hypersensitivity on head and face regions.
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Key Findings & Data:The purpose of this small pilot study was to verify underlying causes of pruritus in cats with head and facial dermatitis by using in vitro Veterinary Polycheck allergy tests which specifically detect Immunoglobulin E (IgE) concentrations against 20 different allergens.
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Conclusion & Impact:Positive reactions in healthy cats might indicate a subclinical hypersensitivity state or the requirement for investigating other relevant factors.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Pruritus is one of the most common clinical findings in cats which have hypersensitivity on head and face regions. The purpose of this small pilot study was to verify underlying causes of pruritus in cats with head and facial dermatitis by using in vitro Veterinary Polycheck allergy tests which specifically detect Immunoglobulin E (IgE) concentrations against 20 different allergens. Ten cats were introduced to the clinic with varying degrees of head and facial dermatitis along with primary/secondary skin lesions and pruritus. At first, haematological, parasitological, microbiological and clinical examinations were performed. Six healthy cats without dermatitis (neither infection nor hypersensitivity) were also evaluated as a control group. Afterwards, 0.2 ml of blood was taken from all cats for specific IgE analysis against 20 different allergens. Regarding allergen specific IgE levels (kU/l) in cats with head/facial dermatitis and in healthy cats, in vitro tests gave a positive reaction most frequently against flea, Acarus siro, D. farinae, Ragweed (Ambrosia), Lambs quarter and Tyrophagus. There was a statistical significance between two groups for all allergens as follows; Lepidoglyphus (p=0.031), Alternaria/Cladosporium (p=0.011), Stinging nettle (p=0.011) Lambs quarter (p=0.031), Sorrel (p=0.003) and flea (Ctenocephalides) (p=0.031). In the present study, all positive reactions on the Polycheck test were thought to indicate underlying allergens of facial/head dermatitis in cats. It has to be mentioned that, in vitro allergy tests cannot be used as a sole method for distinguishing hypersensitivity from healthy cats due to the clinically irrelevant findings regarding morphology in healthy cats. Positive reactions in healthy cats might indicate a subclinical hypersensitivity state or the requirement for investigating other relevant factors.

Özet

Aşırı duyarlılığı olan kedilerde baş ve yüzde kaşıntı en sık görülen klinik bulgulardan biridir. Bu pilot çalışmanın amacı, İmmunoglobulin E (IgE) konsantrasyonlarını 20 farklı allerjene karşı spesifik olarak saptayan in vitro Polycheck Veteriner alerji testi kullanılarak baş ve yüz dermatitis bulunan kedilerde kaşıntının altta yatan nedenlerini açıklığa kavuşturmaktır. Baş ve yüz dermatiti yanında çeşitli derecelerde primer/sekonder deri lezyonları ve kaşıntısı bulunan 10 kedi kliniğe getirildi. İlk aşamada hematolojik, parazitolojik, mikrobiyolojik ve klinik muayeneler gerçekleştirildi. Aynı zamanda karşılaştırmalı olarak dermatit bulguları olmayan (enfeksiyöz ya da hipersensivite) 6 sağlıklı kedi kontrol grubu olarak değerlendirildi. Daha sonra tüm kedilerden 20 farklı allerjene karşı spesifik IgE analizi için 0.2 ml kan örneği alındı. Baş/yüz dermatiti olan kedilerdeki allerjen spesifik IgE düzeyleri (kU/l) sağlıklı kediler ile karşılaştırıldığında in vitro testlerde en sık pire, Acarus siro, D. farinae, Ragweed (Ambrosia), sirken otu ve Tyrophagus’a karşı reaksiyon belirlendi. İki gruptaki sonuçlar karşılaştırıldığında Lepidoglyphus (p=0.031), Alternaria / Cladosporium (p=0.011), ısırgan otu (p=0.011) sirken otu (p=0.031), kuzukulağı (p=0.003) ve pire’ye (Ctenocephalides) (p=0.031) karşı elde edilen değerlerde istatistiksel olarak anlamlı bir fark vardı. Bu çalışmada, Polycheck testinde belirlenen pozitif reaksiyonların kedilerdeki baş/yüz dermatitinin altında yatan alerjenleri gösterdiği düşünülmektedir. Bununla birlikte sağlıklı kedilerde klinik bulguların düzensiz morfolojisi nedeniyle in vitro allerji testlerinin hipersensitiviteyi ayırmada tek yöntem olarak kullanılamayacağı belirtilmelidir. Sağlıklı kedilerde belirlenen artışlar subklinik hipersensitivite durumunu veya diğer ilgili faktörlerin araştırılması gerekliliğini gösterebilir.

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Cite This Article

GÜLTEKİN, M., ERDOĞAN, H., URAL, K. (2022). Allergen specific IgE determination by in vitro allergy test in head and facial feline dermatitis: A pilot study. Ankara Üniversitesi Veteriner Fakültesi Dergisi, Cilt: 65 - Sayı: 4, 379–386. https://ajindex.com/articles/444608

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