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Journals / Ankara Üniversitesi Veteriner Fakültesi Dergisi / 2018 / Cilt: 65 - Sayı: 4

Phylogenetic Analysis of Salmonella Infantis Strains from Poultry by Multilocus Sequence Typing

Kanatlı kökenli Salmonella Infantis suşlarının multilokus dizi tiplendirmesi ile filogenetik analizi

Seyyide SARIÇAM
Hamit Kaan MÜŞTAK
Pages
407–411
Publication Date
2022-10-12
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Serotype information of ST2015 was registered to the MLST database for S.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:In this study, it was aimed to evaluate the 20 Salmonella Infantis strains isolated from broiler chicken hutches (base, dust, environment, rodent traps, feed and water samples) by Multilocus Sequence Typing (MLST).
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Key Findings & Data:Infantis strains were found as ST32 as a result of MLST analysis performed on 7 housekeeping genes belonging to each subspecies and ST32 was thought to be the dominant sequence type for S.
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Conclusion & Impact:Serotype information of ST2015 was registered to the MLST database for S.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

In this study, it was aimed to evaluate the 20 Salmonella Infantis strains isolated from broiler chicken hutches (base, dust, environment, rodent traps, feed and water samples) by Multilocus Sequence Typing (MLST). Six different serovars were used to exhibit the evolutionary relationship between different sequence types (ST) and serotypes on phylogenetic tree. All S. Infantis strains were found as ST32 as a result of MLST analysis performed on 7 housekeeping genes belonging to each subspecies and ST32 was thought to be the dominant sequence type for S. Infantis in Turkey. The other serotypes; S. Enteritidis, S. Typhimurium, S. Hadar, S. Agona, S. Mbandaka and S. Coeln was found to be ST11, ST19, ST33, ST13, ST413 and ST2015, respectively. In this study, S. Agona, S. Mbandaka and S. Coeln serotypes were typed with the MLST for the first time in Turkey. Serotype information of ST2015 was registered to the MLST database for S. Coeln serotype.

Özet

Bu çalışmada broyler tavuk kümeslerinin altlık, toz, çevresel, kemirici kapanları, yem ve su örneklerinden izole edilen 20 adet Salmonella Infantis suşunun multilokus dizi tiplendirme (MLST) ile tiplendirilmesi amaçlandı. Sekans tipleri (ST) ve serotipler arasındaki evrimsel ilişkiyi göstermek amacıyla yapılan filogenetik analizde, 6 farklı serotip çalışmaya dahil edildi. Her bir suşa ait 7 housekeeping gen üzerinden gerçekleştirilen MLST analizi sonucunda tüm S. Infantis suşları ST32 bulundu ve Türkiye’de S. Infantis için baskın sekans tipi olabileceği sonucuna varıldı. S. Enteritidis ST11, S. Typhimurium ST19, S. Hadar ST33, S. Agona ST13, S. Mbandaka ST413 ve S. Coeln ST2015 bulundu. S. Agona, S. Mbandaka ve S. Coeln serotipleri Türkiye’de ilk kez bu çalışmada, MLST ile tiplendirildi. İlk kez bu çalışma ile ST2015 için serotip bilgisi S. Coeln olarak MLST veritabanına girildi.

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Cite This Article

SARIÇAM, S., MÜŞTAK, H. K. (2022). Phylogenetic Analysis of Salmonella Infantis Strains from Poultry by Multilocus Sequence Typing. Ankara Üniversitesi Veteriner Fakültesi Dergisi, Cilt: 65 - Sayı: 4, 407–411. https://ajindex.com/articles/444592

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References (14)

  1. Acar S, Bulut E, Durul B ve ark. (2017): Phenotyping and genetic characterization of Salmonella enterica isolates from Turkey revealing arise of different features specific to geography. Int J Food Micro, 241, 98-107
  2. Achtman M, Wain J, Weil FX ve ark. (2012): Multilocus Sequence Typing as a replacement for serotyping in Salmonella enterica. Plos Pathog, 8, 15-16
  3. Almeida F, Pitondo-Sılva A, Aparecida OM ve ark. (2013): Molecular epidemiology and virulence markers of Salmonella Infantis isolated over 25 years in São Paulo State, Brazil. Infect Genet Evol, 19, 145-151
  4. Andrei A, Zervos MJ (2006): The application of molecular techniques to the study of hospital infection. Arch Pathol Lab Med, 130, 662-668
  5. Antunes P, Mourão J, Machado J ve ark. (2011): First description of qnrS1 IncN plasmid in a ST11 Salmonella Enteritidis clinical isolate from Portugal. Diagn Micr Infec Dis, 69, 463–465
  6. Bado I, García-Fulgueiras V, Cordeiro NF ve ark. (2012): First Human Isolate of Salmonella enterica Serotype Enteritidis Harboring blaCTX-M-14 in South America. Antimicrob Agents Ch, 56, 2132-2134
  7. Cai Y, Tao J, Jiao Y ve ark. (2016): Phenotypic characteristics and genotypic correlation between Salmonella isolates from a slaughterhouse and retail markets in Yangzhou, China. Int J Food Microbiol, 222, 5664
  8. Carattoli A, Filetici E, Villa L ve ark. (2002): Antibiotic Resistance Genes and Salmonella Genomic Island 1 in Salmonella enterica Serovar Typhimurium Isolated in Italy. Antimicrob Agents Ch, 46, 2821–2828
  9. David M, Aanensen DM, Spratt BG (2005): The Multilocus Sequence Typing Network: mlst.net. Nucl Acids Res, 33, 772-733
  10. Dione M (2010): Epidemiology of Non-Typhoidal Salmonella (Nts) in Humans and Animals in the Gambia and Senegal. Tropıcultura, 28, 253-256
  11. Durul B, Acar S, Bulut E ve ark. (2015): Subtyping of Salmonella food isolates suggests the geographic clustering of serotype Telaviv. Foodborne Pathog Dis, 12, 1-4
  12. EFSA (2012): Scientific Opinion of the Panel on Biological Hazards on a request from the European Commission on special measures to reduce the risk for consumers through Salmonella in table eggs-e.g. cooling of table eggs. EFSA J, 957, 1-19
  13. Feasey NA, Dougan G, Kingsley RA ve ark. (2012): Invasive non-typhoidal Salmonella disease: an emerging and neglected tropical disease in Africa. Lancet, 379, 2489–2499
  14. Felsenstein J (1985): Confidence limits on phylogenies: An approach using the bootstrap. Evolution