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Journals / Ankara Üniversitesi Veteriner Fakültesi Dergisi / 2018 / Cilt: 65 - Sayı: 4

In vitro anti-leishmanial activity of Sarcopoterium spinosum against Leishmania tropica

Sarcopoterium spinosum’un Leishmania tropica’ya karşı in vitro anti-leishmanial etkisi

Buket BOZKURT
Mert DÖŞKAYA
Hüsniye KAYALAR
Seray TÖZ
Hüseyin CAN
Şengül CAN GENÇ
Pages
373–377
Publication Date
2022-10-12
DOI
—
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

In conclusion, S. spinosum may be a potential source for the development of novel therapeutic agents to treat L. tropica infection.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Complex clinical symptoms such as ulcerative skin lesions, destructive mucosal inflammation, and disseminated visceral infection can reveal in leishmaniasis.
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Key Findings & Data:After 72 hours of incubation, the growth of L. tropica promastigotes was significantly inhibited and the percentage of inhibition ranged between 42.8 and 100 %.
💡
Conclusion & Impact:In conclusion, S. spinosum may be a potential source for the development of novel therapeutic agents to treat L. tropica infection.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Complex clinical symptoms such as ulcerative skin lesions, destructive mucosal inflammation, and disseminated visceral infection can reveal in leishmaniasis. The conventional drugs are toxic and expensive. In addition, patients receive a long treatment with these drugs which have adverse effects and unfortunately there are some limitations during the treatment. The aim of this study is to investigate the in vitro anti-leishmanial activities of four different extracts of Sarcopoterium spinosum against Leishmania tropica. Initially, different concentrations of ethanol, methanol, n-hexane, and water extracts of S. spinosum were incubated with L. tropica promastigotes. After 72 hours of incubation, the growth of L. tropica promastigotes was significantly inhibited and the percentage of inhibition ranged between 42.8 and 100 %. Among these extracts, the most efficient growth inhibition (100 %) was obtained with methanol extract (at a dose of 50 μg/ml). In conclusion, S. spinosum may be a potential source for the development of novel therapeutic agents to treat L. tropica infection.

Özet

Leishmaniasis’de ülseratif cilt lezyonları, yıkıcı mukozal inflamasyon ve dissemine visseral enfeksiyon gibi kompleks klinik semptomlar ortaya çıkabilmektedir. Konvansiyonel ilaçlar toksik ve pahalıdır. Bunun yanında, hastalar yan etkilere sahip bu ilaçlar ile uzun süre tedavi edilmekte ve maalesef tedavide bazı kısıtlılıklar bulunmaktadır. Bu nedenle, bu çalışmada Sarcopoterium spinosum’un dört farklı ekstresinin L. tropica’ya karşı in vitro anti-leishmanial etkilerinin araştırılması amaçlanmıştır. İlk olarak, farklı konsantrasyonlardaki S. spinosum’un etanol, metanol, n-hekzan ve su ekstreleri L. tropica promastigotları ile inkübe edilmiştir. İnkübasyonun 72 saat sonrasında, tüm ekstreler L. tropica gelişimini %42.8 ve %100 arasındaki değişen oranlarda önemli derecede inhibe etmiştir. Ekstreler arasında, en etkili gelişim inhibisyonu (%100) metanol ekstresi (50 μg/ml) ile elde edilmiştir. Sonuç olarak, S. spinosum, L. tropica enfeksiyonunu tedavi etmek için yeni teröpatik ajanların geliştirilmesinde potansiyel bir kaynak olabilecektir.

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Cite This Article

BOZKURT, B., DÖŞKAYA, M., KAYALAR, H., TÖZ, S., CAN, H., GENÇ, Ş. C. (2022). In vitro anti-leishmanial activity of Sarcopoterium spinosum against Leishmania tropica. Ankara Üniversitesi Veteriner Fakültesi Dergisi, Cilt: 65 - Sayı: 4, 373–377. https://ajindex.com/articles/444559

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