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Journals / Ankara Üniversitesi Veteriner Fakültesi Dergisi / 2020 / Cilt: 67 - Sayı: 2

PCR detection of Mycobacterium genavense DNA in fecal samples of caged birds

Kafes kuşu dışkı örneklerinde Mycobacterium genavense DNA’sının PZR ile saptanması

Orkun BABACAN
Bülent BAŞ
Barış SAREYYÜPOĞLU
Pages
201–204
Publication Date
2022-10-12
DOI
—
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

As a conclusion of this study, which is the first announcement of the detection of M. genavense DNA in fecal samples of caged birds in Turkey, PCR was seen to be a rapid, sensitive, and a reliable method in detection of avian mycobacteriosis

📌 3 Key Takeaways

🎯
Objective & Hypothesis:In this study, pathogenic mycobacteria were investigated in fecal samples of caged birds by PCR.
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Key Findings & Data:A total of 47 fecessamples collected from 4 different aviaries in Ankara.
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Conclusion & Impact:As a conclusion of this study, which is the first announcement of the detection of M. genavense DNA in fecal samples of caged birds in Turkey, PCR was seen to be a rapid, sensitive, and a reliable method in detection of avian mycobacteriosis
🔬Design:Lab & Field Experiment
📍Scope:5 samples
⚙️Variables:Domain Parameters

Abstract

In this study, pathogenic mycobacteria were investigated in fecal samples of caged birds by PCR. A total of 47 fecessamples collected from 4 different aviaries in Ankara. DNA extraction from fecal samples was performed with a commercial kit usingspin column technology. PCR was performed with designed primers respectively amplifying 274 base pairs (bp), 128 bp, 102 bp and 219 bp nucleotide sequences of specific genes (16SrRNA, ISI245, IS901 and hypothetical 21kDa protein gene) of Mycobacterium spp.,Mycobacterium avium complex (MAC), Mycobacterium avium subsp. Avium and Mycobacterium genavense, respectively. Five samples were positive and harbored the sequence for the Mycobacterium spp., of 4 of these 5 samples was identified as M. genavense by PCR. As a conclusion of this study, which is the first announcement of the detection of M. genavense DNA in fecal samples of caged birds in Turkey, PCR was seen to be a rapid, sensitive, and a reliable method in detection of avian mycobacteriosis

Özet

Bu çalışmada, kafes kuşlarının dışkı örneklerinde PCR ile patojenik mikobakterilerin varlığı incelendi. Çalışmanın materyalini Ankara'da 4 farklı kuşhaneden toplanan 47 dışkı örneği oluşturdu. Dışkı örneklerinden DNA ekstraksiyonu, spin kolon teknolojisi kullanılarak ticari DNA ekstraksiyon kiti ile yapıldı. PCR, Mycobacterium spp., Mycobacterium avium complex (MAC), Mycobacterium avium subsp. avium ve Mycobacterium genavense’nin spesifik genlerinin (16SrRNA, ISI245, IS901 ve hipotetik 21kDa protein geni) sırasıyla 274 baz çiftini (bp), 128 bp, 102 bp ve 219 bp nükleotit sekanslarını çoğaltan tasarlanmış primerlerle gerçekleştirildi. PZR sonucunda, 5 dışkı örneği Mycobacterium spp. için pozitif bulundu. Örneklerin 4'ü PZR’da M. genavense olarak tanımlandı. Türkiye'deki kafes kuşlarının dışkı örneklerinde M. genavense DNA'sının saptanmasına yönelik ilk duyuru olan bu çalışmanın sonucu olarak, PZR'nin kanatlı mikobakteriyozisi tespitinde hızlı, duyarlı ve güvenilir bir yöntem olduğu görülmüştür.

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Cite This Article

BABACAN, O., BAŞ, B., SAREYYÜPOĞLU, B. (2022). PCR detection of Mycobacterium genavense DNA in fecal samples of caged birds. Ankara Üniversitesi Veteriner Fakültesi Dergisi, Cilt: 67 - Sayı: 2, 201–204. https://ajindex.com/articles/444479

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