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Journals / Ankara Üniversitesi Veteriner Fakültesi Dergisi / 2020 / Cilt: 67 - Sayı: 2

Insulin-like growth factor-1 gene expression in the endometrium of repeat breeder cows after the administration of presynch-10 and ovsynch protocol

Presynch-10 ve ovsynch protokolleri uygulamasından sonra repeat breeder ineklerde endometriyal insülin benzeri büyüme faktörü-1 gen ekspresyonu

Ali Reha AĞAOĞLU
Özgecan KORKMAZ AĞAOĞLU
Özge SİDEKLİ
Mesih KOCAMÜFTÜOĞLU
Pages
193–199
Publication Date
2022-10-12
DOI
—
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

In conclusion,the expression of the IGF-1 gene in the endometrial tissue of RBS cows with SE was identified, but the evidence suggests that the synchronization protocols in question had no significant effect on the gene expression profile being investigated.

📌 3 Key Takeaways

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Objective & Hypothesis:The aim of this study was to investigate the effects of using presynch-10 and ovsynch synchronization protocols onendometrial expression and serum levels of insulin-like growth factor-1 (IGF-1) that thought to play roles during endometrial healing process in cows diagnosed with repeat breeder syndrome (RBS) and subclinical endometritis (SE).
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Key Findings & Data:A total of 40 Holstein-Friesian cowsidentified as having RBS and SE were used.
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Conclusion & Impact:In conclusion,the expression of the IGF-1 gene in the endometrial tissue of RBS cows with SE was identified, but the evidence suggests that the synchronization protocols in question had no significant effect on the gene expression profile being investigated.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

The aim of this study was to investigate the effects of using presynch-10 and ovsynch synchronization protocols onendometrial expression and serum levels of insulin-like growth factor-1 (IGF-1) that thought to play roles during endometrial healing process in cows diagnosed with repeat breeder syndrome (RBS) and subclinical endometritis (SE). A total of 40 Holstein-Friesian cowsidentified as having RBS and SE were used. The animals in Group 1 (G1, n=20) were applied presynch-10 and ovsynch furthermore, biopsy and blood samples were collected both before and after administration. Biopsy and blood samples were taken from the animals in Group 2 (G2, n=20) without performing any synchronization. The IGF-1 gene expression profiles were determined in all samples using RT-qPCR. Even though the level of IGF-1 expression increased in samples taken after synchronization in G1, it was not statistically significant (P>0.05). There was no statistical difference in serum IGF-1 levels between G1 and G2 (P>0.05). In conclusion,the expression of the IGF-1 gene in the endometrial tissue of RBS cows with SE was identified, but the evidence suggests that the synchronization protocols in question had no significant effect on the gene expression profile being investigated.

Özet

Bu çalışmada; repeat breeder sendromu (RBS) olan ve subklinik endometritis (SE) olduğu belirlenen ineklerde uygulanan presynch-10 ve ovsynch senkronizasyon protokollerinin, endometriyal iyileşme sürecinde rol oynadığı düşünülen insülin benzeri büyüme faktörü-1’in (IGF-1) endometriyal ekspresyonu ve serum düzeyleri üzerine etkilerinin araştırılması amaçlanmıştır. Çalışmada RBS olan ve SE tespit edilmiş toplam 40 baş Holstein-Friesian inek kullanılmıştır. Grup 1’de bulunan hayvanlara (G1, n=20), presynch10 ve ovsynch uygulanmış, ayrıca uygulama öncesi ve sonrasında biyopsi ve kan örnekleri alınmıştır. Grup 2’de bulunan hayvanlara (G2, n=20) herhangi bir senkronizasyon uygulaması yapılmadan sadece biyopsi ve kan örnekleri alınmıştır. Tüm örneklerde RT-qPCR ile IGF-1 geninin ekspresyon profilleri belirlenmiştir. G1’de senkronizasyon sonrası alınan örneklerde IGF-1 ekspresyon düzeyinde bir yükselme şekillenmiş olsa da istatistiksel olarak bir önem bulunamamıştır (P>0,05). G1 ve G2 arasında serum IGF-1 seviyesi açısından da istatistiksel bir fark oluşmamıştır (P>0,05). Sonuç olarak; SE tespit edilen RBS’li ineklerin endometriyal dokularında IGF-1 geninin eksprese olduğu belirlenmiş ancak kullanılan senkronizasyon protokollerinin, incelenen genin ekspresyon profili üzerine anlamlı bir etkisinin olmadığı sonucuna varılmıştır

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Cite This Article

AĞAOĞLU, A. R., AĞAOĞLU, Ö. K., SİDEKLİ, Ö., KOCAMÜFTÜOĞLU, M. (2022). Insulin-like growth factor-1 gene expression in the endometrium of repeat breeder cows after the administration of presynch-10 and ovsynch protocol. Ankara Üniversitesi Veteriner Fakültesi Dergisi, Cilt: 67 - Sayı: 2, 193–199. https://ajindex.com/articles/444464

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