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Journals / Ankara Üniversitesi Veteriner Fakültesi Dergisi / 2021 / Cilt: 68 - Sayı: 3

Molecular detection and phylogenetic analysis of Theileria equi and Babesia caballi in wild horses in Konya province of Turkey

Türkiye’nin Konya yöresinde yaban atlarında Theileria equi ve Babesia caballi'nin moleküler tespiti ve filogenetik analizi

Gonca SÖNMEZ
Bilal DİK
Onur CEYLAN
Özlem DERİNBAY EKİCİ
Ceylan CEYLAN
Asma SEMASSEL
Pages
275–281
Publication Date
2022-10-12
DOI
—
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

The results of sequence analysis showed 99.25-100% and 98.23-99.59% nucleotide sequence identity to the previously reported T. equi and B. caballi 18S rRNA gene sequences, respectively.Consequently, the existence of equine piroplasmosis in wild horses was reported for the first time in Turkey, and high molecularprevalences of T. equi and B. caballi were reported with this study

📌 3 Key Takeaways

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Objective & Hypothesis:The aim of this study was to investigate equine piroplasms of wild horses (Equus ferus caballus) in Konya provinceof Turkey in November-December 2017.
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Key Findings & Data:For this aim, blood samples were collected from 36 wild horses and examined for equinepiroplasms by microscopy and multiplex PCR.
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Conclusion & Impact:The results of sequence analysis showed 99.25-100% and 98.23-99.59% nucleotide sequence identity to the previously reported T. equi and B. caballi 18S rRNA gene sequences, respectively.Consequently, the existence of equine piroplasmosis in wild horses was reported for the first time in Turkey, and high molecularprevalences of T. equi and B. caballi were reported with this study
🔬Design:Research Article
📍Scope:2017 Verileri
⚙️Variables:Domain Parameters

Abstract

The aim of this study was to investigate equine piroplasms of wild horses (Equus ferus caballus) in Konya provinceof Turkey in November-December 2017. For this aim, blood samples were collected from 36 wild horses and examined for equinepiroplasms by microscopy and multiplex PCR. Some of the PCR products from positive samples were also sequenced. Five (13.89%)out of the 36 horses were infected with either Theileria equi, Babesia caballi or both in the microscopical examination. Single infectionswith T. equi and B. caballi were detected in three (8.33%) and one horses (2.78%), respectively. Prevalence of T. equi, B. caballi andmix infections was determined as 50%, 38.8% and 38.8% by multiplex PCR, respectively. Multiplex PCR was found more sensitivethan microscopical examination to detect the piroplasms of horses. The results of sequence analysis showed 99.25-100% and 98.23-99.59% nucleotide sequence identity to the previously reported T. equi and B. caballi 18S rRNA gene sequences, respectively.Consequently, the existence of equine piroplasmosis in wild horses was reported for the first time in Turkey, and high molecularprevalences of T. equi and B. caballi were reported with this study

Özet

Bu çalışma Türkiye’nin Konya yöresinde yaban atlarının (Equus ferus caballus) equine piroplasmlarını tespit etmek amacıyla Kasım-Aralık 2017 tarihlerinde yürütülmüştür. Bu amaçla, 36 yaban atına ait kan örnekeri toplanmış ve bu örnekler mikroskobi ve multiplex PCR yöntemi ile equine piroplasmlar yönünden incelenmiştir. Ayrıca pozitif olduğu tespit edilen bazı örneklere ait PCR ürünleri sekanslanmıştır. Mikroskobik incelemede 36 atın 5’i (13,89%) Theileria equi, Babesia caballi veya her ikisiyle birlikte enfekte bulunmuştur. Üç (8,33%) atta T. equi, bir (2,78%) atta da B. caballi tek tür olarak tespit edilmiştir. T. equi ve B. caballi ve miks enfeksiyonların prevalansları multiplex PCR ile sırasıyla %50, %38,8 ve %38,8 olarak belirlenmiştir. Çalışmada kan parazitlerinin tespitinde multiplex PCR yönteminin mikroskobik incelemeden daha duyarlı olduğu görülmüştür. Sekans analizleri sonucunda T. equi ve B. caballi 18S rRNA genleri ile sırasıyla 99,25-100% ve 98,23-99,59%’luk benzerlik tespit edilmiştir. Bu çalışma Türkiye’deki yaban atlarında equine piroplasmosis’in varlığını bildiren ilk çalışmadır ve çalışmada T. equi ve B. caballi’nin moleküler prevalansları yüksek düzeyde tespit edilmiştir.

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Cite This Article

SÖNMEZ, G., DİK, B., CEYLAN, O., EKİCİ, Ö. D., CEYLAN, C., SEMASSEL, A. (2022). Molecular detection and phylogenetic analysis of Theileria equi and Babesia caballi in wild horses in Konya province of Turkey. Ankara Üniversitesi Veteriner Fakültesi Dergisi, Cilt: 68 - Sayı: 3, 275–281. https://ajindex.com/articles/444447

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References (14)

  1. Acici M, Umur S, Guvenc T, et al (2008): Seroprevalence of equine babesiosis in the Black Sea region of Turkey. Parasitol Int, 57, 198-200
  2. Akkan HA, Karaca M, Tutuncu M, et al (2003): Serologic and microscopic studies on babesiosis in horses in the Eastern border of Turkey. J Equine Vet Sci, 23, 181– 183
  3. Aksoy AR (2016): At yetiştiriciliği ders notları. Available at https://docplayer.biz.tr/22854819-At-yetistiriciligi-dersnotlari-yrd-doc-dr-ali-riza-aksoy-1993-guncelleme-mayis2016.html. (Accessed March 26, 2020)
  4. Alhassan A, Pumidonming W, Okamura M, et al (2005): Development of a single round and multiplex PCR method for the simultaneous detection of Babesia caballi and Babesia equi in horse blood. Vet Parasitol, 129, 43–49
  5. Allsopp MT, Allsopp BA (2006): Molecular sequence evidence for the reclassification of some Babesia species. Ann N Y Acad Sci, 1081, 509–517
  6. Allsopp MTEP, Lewis BD, Penzhorn BL (2007): Molecular evidence for transplacental transmission of Theileria equi from carrier mares to their apparently healthy foals. Vet Parasitol, 148, 130–136
  7. Balkaya I, Erdogmus Z (2006): Investigation of prevalence of Babesia equi (Laveran, 1901) and Babesia caballi (Nuttall 1910) in horses by serological methods in Elazığ and Malatya province. Fırat Üniv Sag Bil Derg, 20, 61–63
  8. Baptista AP, Franco C, Mendes M, et al (2010): Subunit composition of Rhodothermus marinus respiratory complex I. Anal Biochem, 407, 104–110
  9. Baptista C, Lopes MS, Tavares AC, et al (2013): Diagnosis of Theileria equi infections in horses in the Azores using cELISA and nested PCR. Ticks Tick Borne Dis, 4, 242–245
  10. Bashiruddin JB, Cammà C, Rebêlo E (1999): Molecular detection of Babesia equi and Babesia caballi in horse blood by PCR amplification of part of the 16s rRNA gene. Vet Parasitol, 84, 75–83
  11. Bhoora R (2009): Molecular characterization of Babesia caballi and Theileria equi, the aetiological agents of equine piroplasmosis, in South Africa. PhD. Thesis. University of Pretoria, South Africa
  12. Bhoora R, Quan M, Franssen L, et al (2010): Development and evaluation of real-time PCR assays for the quantitative detection of Babesia caballi and Theileria equi infections in horses from South Africa. Vet Parasitol, 168, 201-211
  13. Boldbaatar D, Xuan X, Battsetseg B, et al (2005): Epidemiological study of equine piroplasmosis in Mongolia. Vet Parasitol, 127, 29-32
  14. Bose R, Jorgensen WK, Dalgliesh RJ, et al (1995): Current state and future