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Journals / Ankara Üniversitesi Veteriner Fakültesi Dergisi / 2021 / Cilt: 68 - Sayı: 2

Molecular identification using 18S ribosomal RNA of Sarcocystisspp. in bovine minced meat in Van Province, Turkey

Türkiye’nin Van ilindeki sığır kıymalarında Sarcocystis türlerinin 18S ribosomal RNA gen bölgesi kullanılarak moleküler tanımlanması

Bekir OĞUZ
Mustafa Serdar DEĞER
Sena KOŞAL
Pages
97–105
Publication Date
2022-10-12
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

In conclusion, the molecular characterization of Sarcocystis spp. has been provided for the first time in Van Province, and the first unverified scientific data for S. bovini has been established in this study

📌 3 Key Takeaways

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Objective & Hypothesis:This study aimed to determine the prevalence and molecular characterization of Sarcocystis spp. in bovine minced meat that is sold in various grocery stores and butcher shops in Van Province of Turkey.
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Key Findings & Data:For this purpose a total of 150 samples were obtained from İpekyolu, Tuşba, Edremit, Erciş, and Gevaş districts of Van Province in monthly periods from May to October 2019. 28% (42/150) were found positive for Sarcocystis species as a result of the microscopic analyses and 96.6% (145/150) were found positive for Sarcocystis species as a result of the multiplex-PCR and RFLP methods.
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Conclusion & Impact:In conclusion, the molecular characterization of Sarcocystis spp. has been provided for the first time in Van Province, and the first unverified scientific data for S. bovini has been established in this study
🔬Design:Research Article
📍Scope:2019 · 150 samples
⚙️Variables:Domain Parameters

Abstract

This study aimed to determine the prevalence and molecular characterization of Sarcocystis spp. in bovine minced meat that is sold in various grocery stores and butcher shops in Van Province of Turkey. For this purpose a total of 150 samples were obtained from İpekyolu, Tuşba, Edremit, Erciş, and Gevaş districts of Van Province in monthly periods from May to October 2019. 28% (42/150) were found positive for Sarcocystis species as a result of the microscopic analyses and 96.6% (145/150) were found positive for Sarcocystis species as a result of the multiplex-PCR and RFLP methods. Sarcocystis cruzi (96.6%) was detected in all samples that were detected positive using molecular methods. Sarcocystis hominis-like was found in 143 (95%) samples whereas S. hirsuta was detected in only 4 (2.6%) samples. According to the Basic LocalAlignment Search Tool (BLASTN) analysis of the 18S rRNA gene region of the S. cruzi (MN832695) and S. hirsuta (MN832697) isolates, they showed 100% similarity to the samples (MH681972; MH681973) that were submitted to GenBank from China. The BLASTN analysis of the 18S rRNA gene region of the S. hominis-like isolate (MN832696) revealed that it was 99.45% identical to the S. bovini (KT901155) isolated from a water buffalo in New Zealand. In conclusion, the molecular characterization of Sarcocystis spp. has been provided for the first time in Van Province, and the first unverified scientific data for S. bovini has been established in this study

Özet

Bu çalışmanın amacı Türkiye’nin Van ilinde çeşitli market ve kasaplarda satılan sığır kıymalarında Sarcocystis türlerininin yayılışını ve moleküler karakterizasyonlarını ortaya koymaktır. Bu amaçla 2019 yılının Mayıs-Ekim aylarında aylık periyotlarla Van’ın İpekyolu, Tuşba, Edremit, Erciş ve Gevaş ilçelerinden toplam 150 numune toplanmıştır. Mikroskobik analizler sonucunda %28’i (42/150) Sarcocystis türleri yönünden pozitif bulunmuş, multiplex-PZR ve RFLP yöntemleri sonucunda ise %96,6’sı (145/150) Sarcocystis türleri yönünden pozitif bulunmuştur. Moleküler yöntemlerle pozitif olarak belirlenen tüm örneklerde S. cruzi (%96,6) tespit edilmiştir. Sarcocystis hominis-like 143 (%95) örnekte bulunurken, S. hirsuta ise sadece 4 (%2,6) örnekte tespit edilmiştir. Sarcocystis cruzi (MN832695) ve S. hirsuta (MN832697) izolatlarının 18S rRNA gen bölgesinin Basic Local Alignment Search Tool (BLASTN) analizlerine göre, GenBank’a Çin’den kaydedilen (MH681972; MH681973) örneklerle %100 benzerlik göstermiştir. Sarcocystis hominis-like izolatının 18S rRNA gen bölgesinin BLASTN analizlerine göre (MN832696) Yeni Zelanda’da mandadan izole edilen S. bovini (KT901155) izolatı ile %99,45 identik olduğu belirlenmiştir. Sonuç olarak bu çalışma ile Van ilinde ilk kez Sarcocsytis türlerinin moleküler karakterizasyonları sağlanmış ve S. bovini için doğrulanmamış ilk bilimsel veriler oluşturulmuştur.

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Cite This Article

OĞUZ, B., DEĞER, M. S., KOŞAL, S. (2022). Molecular identification using 18S ribosomal RNA of Sarcocystisspp. in bovine minced meat in Van Province, Turkey. Ankara Üniversitesi Veteriner Fakültesi Dergisi, Cilt: 68 - Sayı: 2, 97–105. https://ajindex.com/articles/444439

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References (14)

  1. Araoz V, da Silva Silveira C, More G, et al (2019): Fatal Sarcocystis cruzi-induced eosinophilic myocarditis in a heifer in Uruguay. J Vet Diagn Invest, 31, 656-660
  2. Arslan MÖ, Umur Ş (1997): The prevalence of sarcocystiosis in cattle in Kars and Erzurum Province, Türkiye. Türkiye Parazitol Derg, 21, 417-420
  3. Bertin M, Lemieux D, Rossero A, et al (2014): Sarcocystis hominis is frequently associated with bovine eosinophilic myositis. Renc Rech Ruminants, 21, 321-324
  4. Chiesa F, Muratore E, Dalmasso A, et al (2013): A new molecular approach to assess the occurrence of Sarcocystis spp. in cattle and products thereof: preliminary data. Ital J Food Saf, 2, e41
  5. Domenis L, Peletto S, Sacchi L, et al (2011): Detection of a morphogenetically novel Sarcocystis hominis-like in the context of a prevalence study in semi-intensively bred cattle in Italy. Parasitol Res, 109, 1677-1687
  6. Dubey JP (2015): Foodborne and waterborne zoonotic sarcocystosis. Food Waterborne Parasitol, 1, 2-11
  7. El-Kady AM, Hussein NM, Hassan AA (2018): First molecular characterization of Sarcocystis spp. in cattle in Qena Governorate, Upper Egypt.J Parasit Dis, 42, 114-121
  8. Fayer R, Esposito DH, Dubey JP (2015): Human infections with Sarcocystis species. Clin Microbiol Rev, 28, 295-311
  9. Freiler PF, Mayhew IG, Pollock R (1979): Bovine saccocystosis: pathologic features of naturally occurring infection with Sarcocystis cruzi. Am J Vet Res, 40, 651-657
  10. Gjerde B (2016): Molecular characterisation of Sarcocystis bovifelis, Sarcocystis bovini n. sp., Sarcocystis hirsuta and Sarcocystis cruzi from cattle (Bos taurus) and Sarcocystis sinensis from water buffaloes (Bubalus bubalis). Parasitol Res, 115, 1473-1492
  11. Goncalo JA (2014): Muscle parasites by Sarcocystis spp. and Cysticercus bovis (Taenia saginata) in cattle of the Azores (In Portuguese). PhD Thesis. University of Lisbon, Lisbon, Portugal
  12. Güçlü F, Aldemir OS, Güler L (2004): Differential identification of cattle Sarcocystis spp. by random amplified Polymorphic DNA-Polymerase chain reaction (RAPDPCR). Revue Med Vet, 155, 440-444
  13. Hall TA (1999): BioEdit: A user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser, 41, 95-98
  14. Hamidinejat H, Razi Jalali MH, Nabavi L (2010): Survey on Sarcocystis Infection in Slaughtered Cattle in SouthWest of Iran, Emphasized on Evaluation of Muscle Squash in Comparison with Digestion Method. J Animal Vet Adv,