Journals / Ankara Üniversitesi Veteriner Fakültesi Dergisi / 2021 / Cilt: 68 - Sayı: 4

Determination of antimicrobial and anti-quorum sensing activities of water and ethanol extracts of propolis

Propolisin sulu ve etanol ekstresinin antimikrobiyal ve anti-çoğunluk algılama aktivitesinin belirlenmesi

Pages
373–381
Publication Date
2022-10-12
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Consequently, the study put forward that the phenolic compounds of propolis have antibacterial, anti- quorum sensing, violacein inhibition, and anti-swarming activities changing depend on directly the extraction solvent and phenolic compounds.

📌 3 Key Takeaways

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Objective & Hypothesis:Quorum Sensing is a special system between bacteria to communicate with the signal molecules.
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Key Findings & Data:The antimicrobial activities of the propolis extracts were studied against Escherichia coli ATCC 25922 Gram (-), Listeria monocytogenes ATCC 7644, Streptococcus mutans CNCTC 8/77, Staphylococcus aureus ATCC 25923 Gram (+), Candida albicans ATCC 10239 (yeast) by the well diffusion method.
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Conclusion & Impact:Consequently, the study put forward that the phenolic compounds of propolis have antibacterial, anti- quorum sensing, violacein inhibition, and anti-swarming activities changing depend on directly the extraction solvent and phenolic compounds.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Quorum Sensing is a special system between bacteria to communicate with the signal molecules. Propolis is a resin collected from plants by honey bees. The present study examined anti-quorum sensing and antimicrobial activities of water and ethanol extracts of propolis. The antimicrobial activities of the propolis extracts were studied against Escherichia coli ATCC 25922 Gram (-), Listeria monocytogenes ATCC 7644, Streptococcus mutans CNCTC 8/77, Staphylococcus aureus ATCC 25923 Gram (+), Candida albicans ATCC 10239 (yeast) by the well diffusion method. Anti-quorum sensing activity, violacein inhibition activity, and antiswarming activity were studied using Chromobacterium violaceum CV026 and C. violaceum ATCC 112472, and Pseudomonas aeruginosa (PA01) respectively. Both propolis extracts were detected antibacterial activity against Gram (+) bacteria and Candida albicans. However, antibacterial activity against Gram (-) bacteria were not observed. While the antimicrobial activity of water extract propolis was determined more effectively than ethanol extract, anti-quorum sensing and anti-swarming activities less effective. It was also detected that both extracts display violacein inhibition. It was revealed that the antimicrobial, anti-quorum sensing, anti-swarming activity depended on the dose of propolis extracts. Thirty-six phenolic compounds were analysed of propolis extract by HPLC-DAD. The amount and presence of phenolic compounds in the ethanol extract were more than the water extract apart from benzoic acid, cinnamic acid, syringic acid, (±) epigallocatechin, vitexin, rutin, and trans-chalcone. Consequently, the study put forward that the phenolic compounds of propolis have antibacterial, anti- quorum sensing, violacein inhibition, and anti-swarming activities changing depend on directly the extraction solvent and phenolic compounds.

Özet

Çoğunluğu algılama sistemi bakterilerin sinyal molekülleri ile kendi aralarında iletişim kurmasını sağlayan özel bir sistemdir. Propolis bal arılarının bitkilerden topladığı reçinedir. Bu çalışmada etanol ve su ekstrakt propolisin antimikrobiyal ve antiçoğunluk algılama aktivitesi incelendi. Propolis ekstraktlarının antimikrobiyal aktivitesi Escherichia coli ATCC 25922, Listeria monocytogenes ATCC 7644, Streptococcus mutans CNCTC 8/77, Staphylococcus aureus ATCC 25923 ve Candida albicans ATCC 102392’e karşı disk difüzyon yöntemi ile çalışıldı. Anti-çoğunluk algılama, violacein inhibisyon ve kaynaşma önleyici aktiviteleri sırasıyla Chromobacterium violaceum CV026, C. violaceum ATCC 112472 ve Pseudomonas aeruginosa PA01 ile çalışıldı. Propolis ekstraktlarının Gram (+) bakteriler ve Candida albicans üzerine antimikrobiyal etkisi olmasına rağmen Gram (-) bakterilerde herhangi bir etki tespit edilememiştir. Sulu ektrakt propolisin antibakteriyel aktivitesi etanol ekstrakt propolise göre daha yüksek iken antiçoğunluk algılama ve kaynaşma önleyici aktivitesi daha az olduğu tespit edildi. Violacein inhibisyon aktivitesi ise her iki propolis ekstraktında da tespit edildi. Propolis ekstraktlarının antibakteriyel, anti-çoğunluk algılama ve kaynaşma önleyici aktiviteleri doza bağımlı olarak değiştiği görüldü. Propolis ekstraktlarında otuz altı fenolik bileşik HPLC-DAD ile analiz edildi. Benzoik asit, sinnamik asit, şiringik asit, epigallokateşin, viteksin rutin ve kalkon dışındaki tüm fenolik bileşikler hem miktarı hem de yaygınlığı etanol ekstrakt propoliste daha yüksek olduğu belirlendi. Sonuç olarak bu çalışma propolisin ekstraksiyon solventine ve içeriğindeki fenolik bileşiklere bağlı olarak antimikrobiyal, anti-çoğunluk algılama, violacein inhibisyon ve kaynaşma önleyici aktivitelerinin olduğunu ortaya koydu.

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Cite This Article

CEYLAN, Ö., SORUCU, A. (2022). Determination of antimicrobial and anti-quorum sensing activities of water and ethanol extracts of propolis. Ankara Üniversitesi Veteriner Fakültesi Dergisi, Cilt: 68 - Sayı: 4, 373–381. https://ajindex.com/articles/444426

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