Journals / Ankara Üniversitesi Veteriner Fakültesi Dergisi / 2021 / Cilt: 68 - Sayı: 3

Marine fish parasite, Lernanthropus kroyeri (Copepoda) is a repository of Vibrio anguillarum as evidenced by Loop-Mediated Isothermal Amplification method

Deniz balıkları paraziti, Lernanthropus kroyeri (Copepoda)’nın Vibrio anguillarum için bir depo olabileceğinin Loop-Mediated Isothermal Amplification metodu ile kanıtlanması

Pages
297–300
Publication Date
2022-10-12
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

This study demonstrates evidence that the copepodparasite in the off-host stage on the gills of sea bass is a reservoir of pathogen bacteria, Vibrio anguillarum.

📌 3 Key Takeaways

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Objective & Hypothesis:Fish parasites act as a vector of other pathogenic diseases such as bacteria and virus in fish.
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Key Findings & Data:Our results using the Loopmediated Isothermal Amplification (LAMP) method demonstrated that the bacteria, V. anguillarum is internally present in the off-hostcopepod parasites, L. kroyeri.
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Conclusion & Impact:This study demonstrates evidence that the copepodparasite in the off-host stage on the gills of sea bass is a reservoir of pathogen bacteria, Vibrio anguillarum.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Fish parasites act as a vector of other pathogenic diseases such as bacteria and virus in fish. Although it has been longknown that fish parasites can act as a vector for bacterial pathogens, their role in the transmission of specific bacterial pathogens viaparticular parasites in the off-host stage has been neglected. The aim of the present study was to disclose that if the copepod parasite,Lernanthropus kroyeri in the off-host stage, can store up the aquatic bacterial pathogens: Aeromonas hydrophila, Photobacteriumdamsela subsp. piscicida, Vibrio (Listonella) anguillarum, V. harveyi, V. vulnificus, and V. alginolyticus. Our results using the Loopmediated Isothermal Amplification (LAMP) method demonstrated that the bacteria, V. anguillarum is internally present in the off-hostcopepod parasites, L. kroyeri. It was also found that the parasite, L. kroyeri could survive in the off-host stage for a given time with itspotential of vectoring ability in terms of transmission of the bacterium to the fish. This study demonstrates evidence that the copepodparasite in the off-host stage on the gills of sea bass is a reservoir of pathogen bacteria, Vibrio anguillarum.

Özet

Balık parazitleri bakteri, virus gibi patojenler için vektör olarak hareket edebilmektedirler. Balık parazitlerinin bakteriyel patojenler için vektör olarak hareket ettiği uzun zamandır bilinmesine karşın bazı parazitlerin konakçı (balık) dışındaki dönemlerinde spesifik bakteriyel patojenleri bulaştırmasına ilişkin rolleri yeterince çalışılmamıştır. Bu çalışmadaki temel amacımız bir kopepod parazit olan Lernanthropus kroyeri ‘nin konakçı dışında yaşadığı dönemde sucul bakteriyel patojenlerden: Aeromonas hydrophila, Photobacterium damsela subsp. piscicida, Vibrio (Listonella) anguillarum, V. harveyi, V. vulnificus ve V. alginolyticus’u bünyesinde tutup tutmadığının ortaya çıkarılmasıdır. Loop-mediated Isothermal Amplification (LAMP) metodu ile elde ettiğimiz sonuçlar, araştırılan bakterilerden, V. anguillarum’u kopepod parazit, L. kroyeri’nin taşıdığını göstermektedir. Ayrıca parazit L. kroyeri belli bir süre, balığa bakteri bulaştırma açısından potansiyel vektörel yeteneğini muhafaza ederek konakçı dışında hayatta kalabilmektedir. Bu çalışma levreklerin solungaçlarında bulunan kopepod parazitin konakçı dışında, patojen bakteri V. anguillarum için bir taşıyıcı olduğunu kanıtlamaktadır.

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Cite This Article

YILDIZ, H. Y., OTGUCUOĞLU, Ö. (2022). Marine fish parasite, Lernanthropus kroyeri (Copepoda) is a repository of Vibrio anguillarum as evidenced by Loop-Mediated Isothermal Amplification method. Ankara Üniversitesi Veteriner Fakültesi Dergisi, Cilt: 68 - Sayı: 3, 297–300. https://ajindex.com/articles/444424

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