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Journals / Ankara Üniversitesi Veteriner Fakültesi Dergisi / 2021 / Cilt: 68 - Sayı: 1

Improvement of bovine in vitroembryo production by fetal calf serum and cysteamine supplementation and investigation of freezability

Sığır in vitro embriyo üretiminin fetal buzağı serumu ve sisteamin katkısı ile iyileştirilmesi ve dondurulabilirliğinin araştırılma

Sinem Özlem ENGİNLER
Alper BARAN
Hatice ŞENLİKCİ
Asiye İzem SANDAL
Özen Banu ÖZDAŞ
Kemal AK
Tülay İREZ
Tuğba ELGÜN
Ramazan ARICI
Pages
33–38
Publication Date
2022-10-12
DOI
—
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

The rate of blastocyst in SOF+FCS and CR1aa+FCS were found 17.9% and 15.4% respectively and the difference between groups was statistically significant (P

📌 3 Key Takeaways

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Objective & Hypothesis:The aim of this study was to investigate the effects of cysteamine (Cys) and fetal calf serum (FCS) in synthetic oviduct fluid (SOF) and charles and rosenkrans(CR1aa) on the bovine in vitro embryo production and its ability of freeze.
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Key Findings & Data:The oocytes were divided into two groups and allowed to mature in TCM-199, with and without cysteamine.
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Conclusion & Impact:The rate of blastocyst in SOF+FCS and CR1aa+FCS were found 17.9% and 15.4% respectively and the difference between groups was statistically significant (P
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

The aim of this study was to investigate the effects of cysteamine (Cys) and fetal calf serum (FCS) in synthetic oviduct fluid (SOF) and charles and rosenkrans(CR1aa) on the bovine in vitro embryo production and its ability of freeze. The oocytes were divided into two groups and allowed to mature in TCM-199, with and without cysteamine. They were divided into 4 subgroups according to whether they contain Cys and FCS in 2 different culture media (SOF and CR1aa). Accordingly, 8 groups were formed as SOF+FCS+Cys, SOF+Cys, SOF+FCS, SOF, CR1aa+FCS+Cys, CR1aa+Cys, CR1aa+FCS and CR1aa. Embryos were cultured for 7-9 days and were frozen using vitrification method. Development of embryos was observed during the first 24 hr post-thaw period. The highest rate of compact morula was 20% in SOF+FCS+Cys and 19.4% in CR1aa+FCS+Cys. The rate of blastocyst in SOF+FCS and CR1aa+FCS were found 17.9% and 15.4% respectively and the difference between groups was statistically significant (P

Özet

Bu çalışmanın amacı, sisteamin (Cys) ve fetal buzağı serumu (FCS)’nun sentetik oviduct sıvısı (SOF) ve charles ve rosenkrans (CR1aa) medyumlarında in vitro sığırembriyo üretimi ve donma kabiliyeti üzerindeki etkilerini araştırmaktır. Toplanan oositler iki gruba ayrıldı ve sisteaminli ve sisteaminsiz TCM-199 medyumunda maturasyonuna izin verildi. 2 farklı medyumda (SOF ve CR1aa) kültüre edilmek üzere Cys ve FCS içerip içermediklerine göre 4 alt gruba ayrıldı. Buna göre 8 alt grup; SOF+FCS+Cys, SOF+Cys, SOF+FCS, SOF, CR1aa+FCS+Cys, CR1aa+Cys, CR1aa+FCS ve CR1aa olarak oluşturuldu. Embriyolar 7-9 gün boyunca kültüre edildi ve vitrifikasyon yöntemi kullanılarak donduruldu. Çözdürme sonrası ilk 24 saat boyunca embriyonik gelişim gözlendi. Kompakt morula oranı en yüksek SOF+FCS+Cys'de %20 ve CR1aa+FCS+Cys'de %19,4 olarak bulundu. SOF+FCS ve CR1aa+FCS'deki blastosist oranı sırasıyla %17,9 ve %15,4 olarak saptandı ve gruplar arasındaki fark istatistiksel olarak anlamlıydı (P

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Cite This Article

ENGİNLER, S. Ö., BARAN, A., ŞENLİKCİ, H., SANDAL, A. İ., ÖZDAŞ, Ö. B., AK, K., İREZ, T., ELGÜN, T., ARICI, R. (2022). Improvement of bovine in vitroembryo production by fetal calf serum and cysteamine supplementation and investigation of freezability. Ankara Üniversitesi Veteriner Fakültesi Dergisi, Cilt: 68 - Sayı: 1, 33–38. https://ajindex.com/articles/444414

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References (13)

  1. Abe H, Yamashita S, Itoh T, et al (1999): Ultrastructure of bovine embryos developed from in vitro matured and fertilized oocytes: Comparative morphological evaluation of embryos cultured either in serum free medium or serum supplemented medium. Mol Reprod Dev, 53, 325-335
  2. Aitken RJ, Harkiss D, Buckingham D (1993): Relationship between iron-catalysed lipid peroxidation potential and human sperm function. J Reprod Fertil, 98, 257-265
  3. Anchordoquy JM, Anchordoquy JP, Testa JA, et al (2015): Influence of vascular endothelial growth factor and Cysteamine on in vitro bovine oocyte maturation and subsequent embryo development. Cell Biol Int, 39, 1090- 1098
  4. Balasubramanian S, Rho GJ (2007): Effect of cysteamine supplementation of in vitro matured bovine oocytes on chilling sensitivity and development of embryos. Anim Reprod Sci, 98, 282-292
  5. Do VH, Walton S, Taylor-Robinson AW (2016): Improvements to in vitro culture media for use in bovine IVF. J Vet Sci Anim Husb, 4, 205
  6. Duque P, Gomez E, Diaz E, et al (2003): Use of two serum during bovine embryo culture in vitro. Theriogenology, 59, 889-899
  7. Enginler SO, Ozdaş OB, Sandal AI, et al (2016): The effect of cysteamine and oviductal cells in different culture media on the development of sheep embryos. Kafkas Univ Vet Fak, 22, 139-145
  8. Gomez E, Rodriguez A, Munoz M, et al (2008): Serum free embryo culture medium improves in vitro survival of bovine blastocysts to vitrification. Theriogenology, 69, 1013-1021
  9. Guerin P, El Mouatassim S, Menezo Y (2001): Oxidative stress and protection against reactive oxygen species in the pre-implantation embryo and its surroundings. Hum Reprod Update, 7, 175-189
  10. Hajarian H, Aghaz F, Karami Shabankareh H (2017): Replacement of serum with sericin in in vitro maturation and culture media: Effects on embryonic developmental competence of Sanjabi sheep embryo during breeding season. Theriogenology, 92, 144-148
  11. Hammon DS, Wang S, Holyoak GR (2000): Effects of ammonia during different stages of culture on development of in vitro produced bovine embryos. Anim Reprod Sci, 59, 23-30
  12. Hong J, Lee E (2007): Intrafollicular amino acid concentration and the effect of amino acids in a defined maturation medium on porcine oocyte maturation, fertilization, and preimplantation development. Theriogenology, 68, 728-735
  13. Kharche SD, Agrawal S, Pathak J, et al (2016): Influence of cysteamine supplementation during in vitro culture of early stage caprine em