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Journals / Ankara Üniversitesi Veteriner Fakültesi Dergisi / 2022 / Cilt: 69 - Sayı: 3

Proliferative and apoptotic evaluations of renal preventive effects of coenzyme Q10 in radioiodine-131 induced renal damage

Radioiodine-131 ile uyarılmış böbrek hasarının önlenmesinde koenzim Q10’un proliferatif ve apoptotik etkilerin değerlendirilmesi

Nihat YUMUŞAK
Meliha KORKMAZ
Gökhan KOCA
Aylin AKBULUT
Hasan İkbal ATILGAN
Pages
265–272
Publication Date
2022-10-12
DOI
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AI Summary & Key Takeaways

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⚡ TL;DR(Too Long; Didn't Read)

This histopathological damage was found to be significantly less in CoQ10 group versus RAI group (P

📌 3 Key Takeaways

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Objective & Hypothesis:The aim of this study was to investigated anti-proliferative and anti-apoptotic effects of coenzyme Q10 (CoQ10) in the prevention of radioiodine-131 (RAI) (I131) induced kidney damage.
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Key Findings & Data:A total of 24 Wistar albino rats were separated into equal three groups (n = 8/group): Group 1 (control): untreated group; Group 2 (RAI): 3 mCi/kg RAI oral route; Group 3 (RAI+CoQ10): 3 mCi/kg RAI oral route and intraperitoneally 30 mg/kg/day CoQ10.
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Conclusion & Impact:This histopathological damage was found to be significantly less in CoQ10 group versus RAI group (P
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

The aim of this study was to investigated anti-proliferative and anti-apoptotic effects of coenzyme Q10 (CoQ10) in the prevention of radioiodine-131 (RAI) (I131) induced kidney damage. A total of 24 Wistar albino rats were separated into equal three groups (n = 8/group): Group 1 (control): untreated group; Group 2 (RAI): 3 mCi/kg RAI oral route; Group 3 (RAI+CoQ10): 3 mCi/kg RAI oral route and intraperitoneally 30 mg/kg/day CoQ10. CoQ10 treatment was started two days before RAI administration and was continued five days once daily after RAI. Pathomorphological parameters of kidneys were measured using hematoxylin–eosin and Masson’s trichrome staining. Immunohistochemically; proliferating cell nuclear antigen (PCNA), caspase 8, caspase 9 and terminal deoxynucleotidyl transferase–mediated deoxyuridine triphosphate nick end labeling (TUNEL) were used to determine proliferation and apoptosis. With the exception of the control group, varying degrees of inflammation, degeneration, necrosis, and interstitial/perivascular fibrosis were detected in the kidneys of all rats. This histopathological damage was found to be significantly less in CoQ10 group versus RAI group (P

Özet

Bu çalışmanın amacı radioioidine-131 (RAI) (I131) tedavisine bağlı oluşan böbrek hasarının önlenmesinde koenzim Q10 (CoQ10)’nun anti-apoptotik ve anti-proliferatif etkisinin araştırılmasıdır. Yirmi dört Wistar albino sıçan rastgele olarak üç gruba ayrıldı (n=8/grup): Grup 1 (kontrol): tedavi uygulanmayan grup; Grup 2 (RAI): orogastrik yolla tek doz 3 mCi/kg RAI uygulanan grup; Grup 3 (RAI+CoQ10); tek doz 3 mCi/kg RAI ve intraperitonel olarak 30 mg/kg/gün CoQ10 uygulanan grup. CoQ10 tedavisi RAI uygulamasından 2 gün önce başladı ve RAI sonrası günde bir kez beş gün süreyle uygulandı. Son CoQ10 uygulamasından yirmi dört saat sonra sıçanların böbrekleri alınarak patomorfolojik incelemeler amacıyla hematoksilen-eosin ve Masson’un Trikrom metoduna göre boyandı. İmmunohistokimyasal olarak: proliferasyonun belirlenmesi amacıyla prolifere hücre nükleer antijen (PCNA); apoptozisin belirlenmesi amacıyla Caspase 8 ve Caspase 9; DNA hasarının belirlenmesi amacıyla TUNEL boyaması yapıldı. Çalışma sonucunda; kontrol grubu dışındaki sıçanların böbreklerinde değişen şiddette inflamasyon, ödem, dejenerasyon, nekroz ve fibrozis görülürken bu bulguların CoQ10 ile tedavi edilen grupta istatistiksel olarak belirgin düzeyde daha az olduğu dikkati çekti (P

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Cite This Article

YUMUŞAK, N., KORKMAZ, M., KOCA, G., AKBULUT, A., ATILGAN, H. İ. (2022). Proliferative and apoptotic evaluations of renal preventive effects of coenzyme Q10 in radioiodine-131 induced renal damage. Ankara Üniversitesi Veteriner Fakültesi Dergisi, Cilt: 69 - Sayı: 3, 265–272. https://ajindex.com/articles/444376

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