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Journals / Ankara Üniversitesi Veteriner Fakültesi Dergisi / 2022 / Cilt: 69 - Sayı: 3

Effect of probiotic on mast cell density and expression of tryptase, chymase, and TNF-α in the urinary bladder of rats with high cholesterol

Probiyotiğin, yüksek kolesterolü olan sıçanların idrar kesesinde mast hücre yoğunluğu ve triptaz, kimaz ve TNF-α ekspresyonu üzerine etkisi

Tuğrul ERTUĞRUL
Şerife TÜTÜNCÜ
Gülay ÇİFTCİ
Pages
273–280
Publication Date
2022-10-12
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Serum TC levels were remarkably increased in group 2 compared to the group 1 (P

📌 3 Key Takeaways

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Objective & Hypothesis:In this study, it was aimed to immunohistochemically evaluate the possible effect of probiotic supplementation on rats fed a high cholesterol diet on mast cell heterogeneity and density in the urinary bladder and on the expression of chymase, tryptase and tumor necrosis factor-alpha (TNF-α) cytokine in the urinary bladder.
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Key Findings & Data:For 8 weeks, Group 1 (control group) was fed regular rat diet.
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Conclusion & Impact:Serum TC levels were remarkably increased in group 2 compared to the group 1 (P
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

In this study, it was aimed to immunohistochemically evaluate the possible effect of probiotic supplementation on rats fed a high cholesterol diet on mast cell heterogeneity and density in the urinary bladder and on the expression of chymase, tryptase and tumor necrosis factor-alpha (TNF-α) cytokine in the urinary bladder. Three groups were formed in the study. For 8 weeks, Group 1 (control group) was fed regular rat diet. Food for Group 2 (feeding +2% cholesterol) (high cholestrol group) was provided for 8 weeks. For the last four weeks, Group 3 (%2 cholesterol + Lactobacillus acidophilus) (probiotic group) was administered L. acidophilus probiotics. Levels of total cholesterol (TC) was measured by using a spectrophotometric autoanalyzer. Serum TC levels were remarkably increased in group 2 compared to the group 1 (P

Özet

Bu çalışma da yüksek kolesterollü diyet ile beslenen ratlara probiyotik ilavesinin ratların idrar kesesindeki mast hücre heterojenitesi ve yoğunluğuna olası etkisi ile mesanede kimaz, triptaz ve tümör nekroz faktör-alfa (TNF-α) sitokinin ekspresyonuna etkisinin immunohistochemically değerlendirilmesi amaçladı. Grup 1 (kontrol grubu) 8 hafta boyunca standart fare yemi ile beslendi. Grup 2 (+%2 kolesterolle beslenen) (yüksek kolestrol grubu) 8 hafta boyunca verildi. Grup 3 (%2 kolesterol + Lactobacillus acidophilus) (probiyotik grubu): Son 4 hafta L. acidophilus probiyotiği verildi. Total kolesterol (TC) düzeyi spektrofotometrik yöntemle otoanalizör kullanılarak ölçüldü. Grup 2 serum TC düzeylerinin grup 1’e göre önemli ölçüde arttığı belirlendi (P

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Cite This Article

ERTUĞRUL, T., TÜTÜNCÜ, Ş., ÇİFTCİ, G. (2022). Effect of probiotic on mast cell density and expression of tryptase, chymase, and TNF-α in the urinary bladder of rats with high cholesterol. Ankara Üniversitesi Veteriner Fakültesi Dergisi, Cilt: 69 - Sayı: 3, 273–280. https://ajindex.com/articles/444367

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