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Journals / Ankara Üniversitesi Veteriner Fakültesi Dergisi / 2022 / Cilt: 69 - Sayı: 4

Presence and virulence characterization of Listeria monocytogenes from fish samples in the Black Sea, Turkey

Karadeniz kaynaklı balıklardan izole edilen Listeria monocytogenes suşlarının varlığı ve virülens özelliklerinin incelenmesi

Eren GÖZÜTOK
Ali AYDIN
Pages
387–394
Publication Date
2022-10-12
DOI
—
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

According to the results, serogroup 4b-4d-4e isolated from striped red mullet was found to be unique to raw fish and susceptible to all tested antibiotics.

📌 3 Key Takeaways

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Objective & Hypothesis:Listeria monocytogenes, characterized by a high mortality rate in humans, is a bacterium that causes listeriosis and is found in various aquatic products.
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Key Findings & Data:The aim of this research was to investigate the presence, serotype distribution, virulence factor genes, and antibiotic susceptibility of L. monocytogenes strains isolated from a total of 500 fish samples of whiting (Merlangius merlangus euxinus) (n:243) and striped red mullet (Mullus surmuletus) (n:257) caught in the Black Sea between the years 2013-2014.
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Conclusion & Impact:According to the results, serogroup 4b-4d-4e isolated from striped red mullet was found to be unique to raw fish and susceptible to all tested antibiotics.
🔬Design:Research Article
📍Scope:2013-2014 Verileri
⚙️Variables:Domain Parameters

Abstract

Listeria monocytogenes, characterized by a high mortality rate in humans, is a bacterium that causes listeriosis and is found in various aquatic products. The aim of this research was to investigate the presence, serotype distribution, virulence factor genes, and antibiotic susceptibility of L. monocytogenes strains isolated from a total of 500 fish samples of whiting (Merlangius merlangus euxinus) (n:243) and striped red mullet (Mullus surmuletus) (n:257) caught in the Black Sea between the years 2013-2014. Only one (0.2%) L. monocytogenes strain (striped red mullet) was isolated according to the cultural method (EN ISO 11290-1) and confirmed by PCR analysis. The L. monocytogenes strain was identified as serogroup 4b-4d-4e. Furthermore, the strain harboured hlyA, inlA, inlC, inlJ, plcA, plcB, prfA, mpl, actA, monoA-B, flaA, lip 1-2a, fri, iap, and gtcA genes except the dltA gene. On the other hand, L. monocytogenes strain susceptibility to ampicillin, meropenem, erythromycin, trimetophrim/sulfamethoxazole, and penicillin G was evaluated with the disc-diffusion method. According to the results, serogroup 4b-4d-4e isolated from striped red mullet was found to be unique to raw fish and susceptible to all tested antibiotics. In addition, it is considered that carrying out this research in different seas and with different fish species would be appropriate for determining the prevalence and virulence characteristics of L. monocytogenes.

Özet

Listeria monocytogenes, insanlarda yüksek mortalite oranı ile karakterize listeriozise neden olan bir bakteri olup, su ürünlerinde çeşitli düzeylerde bulunabilmektedir. Bu çalışmanın amacı, Karadenizʼden 2013-2014 yılları arasında avlanan toplam 500 adet Tekir (Mullus surmuletus) (n: 257) ve Mezgit (Merlangius merlangus euxinus) (n: 243) balığından izole edilen L. monocytogenes varlığı, serogrup dağılımı, virülens faktör genler ve antibiyotik duyarlılığını araştırmaktır. Bir adet (%0,2) L. monocytogenes suşu (Tekir balığı) standart kültür yöntemi (EN ISO 11290-1) ile izole edilmiş ve PCR analizi ile doğrulanmıştır. Söz konusu, L. monocytogenes suşu 4b-4d-4e serogrubu olarak tespit edilmiştir. Ayrıca L. monocytogenes suşunun hlyA, inlA, inlC, inlJ, plcA, plcB, prfA, mpl, actA, monoA-B, flaA, lip 1-2a, fri, iap, gtcA genlerini içerdiği, fakat dltA genini içermediği belirlenmiştir. Diğer taraftan, L. monocytogenes suşunun ampicillin, meropenem, erythromycin, trimetophrim/sulfamethoxazole ve penicillin G antibiyotiklerine karşı duyarlılığı disk diffüzyon testi ile ölçülmüştür. Elde edilen sonuçlara göre, Tekir balığından izole edilen 4b-4d-4e serogrubunun çiğ balığa özgü olduğu ve test edilen antibiyotiklerin hepsine duyarlı olduğu bulunmuştur. Ayrıca, bu araştırmanın farklı denizlerde ve farklı balık türleri ile yapılmasının L. monocytogenes ’in prevalans ve virülens özelliklerinin belirlenmesi için uygun olacağı düşünülmektedir.

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Cite This Article

GÖZÜTOK, E., AYDIN, A. (2022). Presence and virulence characterization of Listeria monocytogenes from fish samples in the Black Sea, Turkey. Ankara Üniversitesi Veteriner Fakültesi Dergisi, Cilt: 69 - Sayı: 4, 387–394. https://ajindex.com/articles/444337

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