Journals / Ankara Üniversitesi Veteriner Fakültesi Dergisi / 2022 / Cilt: 69 - Sayı: 3

Investigation of deformed wing virus, black queen cell virus, and acute bee paralysis virus infections in honey bees using reverse transcriptase-polymerase chain reaction (RT-PCR) method

Bal arılarında deforme kanat virus, siyah kraliçe hücre virus ve akut arı felci virus enfeksiyonlarının reverz transkriptaz-polymerase chain reaction (RT-PCR) metodu kullanılarak araştırılması

Pages
303–311
Publication Date
2022-10-12
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

The revealed epidemiological conditions lead to the conclusion that serious measures are needed to control these infections in this region.

📌 3 Key Takeaways

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Objective & Hypothesis:Viruses are one of the most potential risk factors that negatively affect the different life stages of honey bees.
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Key Findings & Data:In our study, the adult bees were taken from 31 different beehives and comb samples that had different honey bee breeds and were sampled randomly from 15 beekeeping areas within the region.
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Conclusion & Impact:The revealed epidemiological conditions lead to the conclusion that serious measures are needed to control these infections in this region.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Viruses are one of the most potential risk factors that negatively affect the different life stages of honey bees. This study was conducted to determine the presence of infections caused by the deformed wing virus (DWV), black queen cell virus (BQCV), and acute bee paralysis virus (ABPV) in honey bees in the beekeeping regions of Burdur, along with obtaining information about their prevalence in this particular region. In our study, the adult bees were taken from 31 different beehives and comb samples that had different honey bee breeds and were sampled randomly from 15 beekeeping areas within the region. The collected samples were analyzed using the reverse transcriptase-polymerase chain reaction (RT-PCR), and the prevalence of DWV, BQCV, and ABPV infections were determined to be 74.19% (23/31), 25.81%, (8/31) and 74.19% (23/31), respectively. In this study, the distribution of positive samples and the rates of multiple infections were determined in the colonies. Of the positive honey bee samples, 12 (%38.71) were detected only for one virus, 9 (%29.03) were positive for two viruses (DWV-ABPV) and 8 (%25.81) were positive for all three viruses. In the present study, the presence of the three bee viruses that caused significant damage to the colonies by multiple infections in the Burdur region was determined with RT-PCR. To our knowledge, this is the first report of three mentioned bee infections in honey bees in the Burdur region. The revealed epidemiological conditions lead to the conclusion that serious measures are needed to control these infections in this region.

Özet

Viruslar bal arılarının farklı yaşam evrelerini olumsuz yönde etkileyen potansiyel risk faktörlerinden birisidir. Bu çalışma, Burdur yöresinde arıcılık işletmelerinde bulunan bal arılarında deforme kanat virus (DKV), siyah kraliçe hücre virus (BKHV) ve akut arı felci virus (AAFV) enfeksiyonlarının varlığının virolojik olarak saptanması ve bu yöredeki yaygınlıkları hakkında bilgi edinilmesi amacıyla yapılmıştır. Araştırmada söz konusu yörede bulunan 15 arıcılık işletmesinden tesadüfi örnekleme ile farklı arı ırklarından 31 farklı kovandan ergin arı ve yavrulu petek örneği usulüne uygun olarak alındı. Toplanan numuneler reverse transcriptase-polymerase chain reaction (RT-PCR) metodu ile analiz edildi ve DKV, BKHV, AAFV enfeksiyonlarının prevalansı sırasıyla %74,19 (23/31), %25,81 (8/31), %74,19 (23/31) oranlarında belirlendi. Çalışmanın yürütüldüğü kolonilerde tespit edilen pozitifliğin ırklara göre dağılımı ve çoklu enfeksiyon oranları tespit edildi. Pozitif örneklerin 12’si (%38,71) sadece bir virusa, 9’u (%29,03) iki virusa (DKVAAFV) ve 8’ide (%25,81) üç virusa karşı pozitif bulundu. Bu çalışmada Burdur yöresinde çoklu enfeksiyonlarla kolonilercde önemli hasara neden olan üç arı virusunun varlığı RT-PCR ile belirlendi. Bu çalışma, Burdur bölgesindeki bal arılarında adı geçen üç virusun varlığına ilişkin bilinen ilk raporu sunmaktadır. Tespit edilen epidemiyolojik sonuçlara göre yörede bu enfeksiyonların kontrolü amacıyla ciddi önlemlerin alınması gerektiği kanaatine varıldı.

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Cite This Article

USTA, A., YILDIRIM, Y. (2022). Investigation of deformed wing virus, black queen cell virus, and acute bee paralysis virus infections in honey bees using reverse transcriptase-polymerase chain reaction (RT-PCR) method. Ankara Üniversitesi Veteriner Fakültesi Dergisi, Cilt: 69 - Sayı: 3, 303–311. https://ajindex.com/articles/444336

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