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Journals / Anadolu Üniversitesi Bilim ve Teknoloji Dergisi :C-Yaşam Bilimleri ve Biyoteknoloji / 2018 / Cilt: 7 - Sayı: 1

CULTIVATION-BASED CHARACTERIZATION of MICROBIAL ASSEMBLAGES in SOLAR SALTERNS, SİVAS

SİVAS’IN SOLAR TUZLALARINDAKİ MİKROBİYAL TOPLULUKLARIN KÜLTÜRBAĞIMLI KARAKTERİZASYONU

Mehmet Burçin MUTLU
Seval ÇINAR
Pages
1–11
Publication Date
2022-10-11
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Halophilic archaeal isolates were distributed among genera Haloferax, Natrinema, Haloterrigena, Halopelagius, Halorhabdus and Haloarcula

📌 3 Key Takeaways

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Objective & Hypothesis:In this study, halophilic microorganisms were isolated from different solar salterns in Sivas and characterization of these isolates based on 16S rRNA gene analysis have been performed.
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Key Findings & Data:Isolation was done by using 5 different media prepared with different salt concentrations. 16S rRNA genes of morphologically distinct colonies were analyzed by ARDRA and restriction profiles were compared. 47 strains were selected for identification purposes.
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Conclusion & Impact:Halophilic archaeal isolates were distributed among genera Haloferax, Natrinema, Haloterrigena, Halopelagius, Halorhabdus and Haloarcula
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

In this study, halophilic microorganisms were isolated from different solar salterns in Sivas and characterization of these isolates based on 16S rRNA gene analysis have been performed. Isolation was done by using 5 different media prepared with different salt concentrations. 16S rRNA genes of morphologically distinct colonies were analyzed by ARDRA and restriction profiles were compared. 47 strains were selected for identification purposes. It has been determined that the isolates in the Bacteria domain are phylogenetically related to genera Idiomarina, Salinivibrio, Halomonas, Salinibacter, Gracilibacillus, Thalassobacillus, Aliifodinibius, Chromohalobacter, Planococcus, Marinobacter, Bacillus, Aquisalimonas, Marinococcus, Alkalibacillus, Kangiella and Microbulbifer. Halophilic archaeal isolates were distributed among genera Haloferax, Natrinema, Haloterrigena, Halopelagius, Halorhabdus and Haloarcula

Özet

Bu çalışmada Sivas’ta bulunan farklı solar tuzlalardan halofilik mikroorganizmaların izolasyonu yapılmıştır ve elde edilenizolatların 16S rRNA gen analizine dayalı karakterizasyonu gerçekleştirilmiştir. İzolasyon işlemi farklı tuz konsantrasyonları ilehazırlanmış 5 farklı besiyeri ile gerçekleştirilmiştir. Morfolojik olarak farklılık gösteren kolonilerin 16S rRNA genleri ARDRA ileanaliz edilmiştir ve kesim profilleri karşılaştırılmıştır. 47 adet suş tanımlama amacıyla seçilmiştir. Bacteria domaini içerisinde yeralan izolatların Idiomarina, Salinivibrio, Halomonas, Salinibacter, Gracilibacillus, Thalassobacillus, Aliifodinibius,Chromohalobacter, Planococcus, Marinobacter, Bacillus, Aquisalimonas, Marinococcus, Alkalibacillus, Kangiella veMicrobulbifer cinsleri ile filogenetik olarak ilişkili olduğu saptanmıştır. Halofilik arke izolatları ise Haloferax, Natrinema,Haloterrigena, Halopelagius, Halorhabdus ve Haloarcula cinsleri içerisinde dağılım göstermiştir

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Cite This Article

MUTLU, M. B., ÇINAR, S. (2022). CULTIVATION-BASED CHARACTERIZATION of MICROBIAL ASSEMBLAGES in SOLAR SALTERNS, SİVAS. Anadolu Üniversitesi Bilim ve Teknoloji Dergisi :C-Yaşam Bilimleri ve Biyoteknoloji, Cilt: 7 - Sayı: 1, 1–11. https://ajindex.com/articles/438566

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