Journals / Iğdır Üniversitesi Fen Bilimleri Enstitüsü Dergisi / 2017 / Cilt: 7 - Sayı: 3

Determination Of Prokaryotic Diversity Of Salda Lake By Next Generation Sequencing Method

Salda Gölü Prokaryotik Çeşitliliğinin Yeni Nesil Dizileme Yöntemiyle Belirlenmesi

Pages
99–106
Publication Date
2017-09-30
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

In addition Halobacteria class, Halobacteriales order, Halobacteriaceae familyincludes Haloarcula, Halobacterium, Halonotius, Halorhabdus, Halorubrum and Haloplanus genus members

📌 3 Key Takeaways

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Objective & Hypothesis:In our study, water sample was taken from Salda Lake and the pH and salinity data for the samplewere determined.
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Key Findings & Data:After that, samples were fltered and DNA extraction was performed.
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Conclusion & Impact:In addition Halobacteria class, Halobacteriales order, Halobacteriaceae familyincludes Haloarcula, Halobacterium, Halonotius, Halorhabdus, Halorubrum and Haloplanus genus members
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Alkaliflik · prokaryotik çeşitlilik · Salda Gölü

Abstract

In our study, water sample was taken from Salda Lake and the pH and salinity data for the samplewere determined. After that, samples were fltered and DNA extraction was performed. The obtained DNA wassequenced by the Illumina MiSeq sequencing system and data was analyzed by the QIIME program. When thedata obtained after the analysis were evaluated, it was determined that the most dominant bacterial phyla areProteobacteria and Actinobacteria. At class level, Gammaproteobacteria, Acidimicrobia and Actinobacteria weredominant. In order level Enterobacteriales, Acidimicrobiales, Actinobacteriales were abundant. When the Archaeadomain was evaluated it was determined that the Euryarchaeota phylum was dominant. Halobacteria memberswere abundant at the class level. In addition Halobacteria class, Halobacteriales order, Halobacteriaceae familyincludes Haloarcula, Halobacterium, Halonotius, Halorhabdus, Halorubrum and Haloplanus genus members

Özet

Çalışmada Salda Gölü’nden örnekleme yapılmış ve örnek için pH ve tuzluluk değerleri belirlenmiştir.Sonrasında örnekler fltreden geçirilerek, bu fltrelerden DNA ekstraksiyonu işlemi yapılmıştır. Elde edilen DNAIllumina MiSeq dizileme sistemiyle dizilenmiş ve veriler QIIME Programı ile analiz edilmiştir. Analiz sonrası eldeedilen veriler değerlendirildiğinde en baskın bulunan Bacteria flumlarının Proteobacteria ve Actinobacteria olduğubelirlenmiştir. Sınıf düzeyinde ise Gammaproteobacteria, Acidimicrobia ve Actinobacteria baskındır. Ordo düzeyindeise Enterobacteriales, Acidimicrobiales, Actinobacteriales baskındır. Archaea domaini değerlendirildiğinde iseEuryarchaeota flumunun baskın olduğu belirlenmiştir. Sınıf düzeyinde Halobacteria üyeleri baskındır. Halobacteriasınıfının Halobacterialaes ordosu Halobacteriaceae familyasının Haloarcula, Halobacterium, Halonotius,Halorhabdus, Halorubrum ve Haloplanus cinsi üyeleri de baskın olarak bulunmaktadır

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Cite This Article

POYRAZ, N., MUTLU, M. B. (2017). Determination Of Prokaryotic Diversity Of Salda Lake By Next Generation Sequencing Method. Iğdır Üniversitesi Fen Bilimleri Enstitüsü Dergisi, Cilt: 7 - Sayı: 3, 99–106. https://ajindex.com/articles/257070

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