Journals / Anadolu Kliniği Tıp Bilimleri Dergisi / 2018 / Cilt: 23 - Sayı: 3

Antimicrobial Activity of the Istranca Oak (Quercus hartwissiana Steven) against Nosocomial Pathogens

Istranca Meşesinin (Quercus hartwissiana Steven) Nozokomiyal Patojenlere Karşı Antimikrobiyal Aktivitesi

Pages
157–162
Publication Date
2022-10-11
DOI
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⚡ TL;DR(Too Long; Didn't Read)

Given that the widest zone diameters were observed against the strains E. coli, S. aureus and C. albicans, we think that it is important to conduct in vivo antimicrobial studies on the inhibitory effects of the Istranca oak indigenous to our country.

📌 3 Key Takeaways

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Objective & Hypothesis:Aim: This study aimed to investigate the antimicrobial activity of extracts from the Istranca oak (Quercus hartwissiana Steven) against bacterial and fungal pathogens.
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Key Findings & Data:After injecting 50 μl of these extracts into sterile discs, the discs were dried in a sterile environment for 24 hours in order for the results not to be affected by the solvents present in the extracts.
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Conclusion & Impact:Given that the widest zone diameters were observed against the strains E. coli, S. aureus and C. albicans, we think that it is important to conduct in vivo antimicrobial studies on the inhibitory effects of the Istranca oak indigenous to our country.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Aim: This study aimed to investigate the antimicrobial activity of extracts from the Istranca oak (Quercus hartwissiana Steven) against bacterial and fungal pathogens. Materials and Methods: Extraction of the Istranca oak was carried out with ethanol, formaldehyde, acetone, ethyl acetate, and methanol solvents. After injecting 50 μl of these extracts into sterile discs, the discs were dried in a sterile environment for 24 hours in order for the results not to be affected by the solvents present in the extracts. Antimicrobial activity against the species Pseudomonas aeruginosa, Acinetobacter baumannii, Escherichia coli, Klebsiella pneumoniae, Salmonella spp., methicillin-resistant Staphylococcus aureus, methicillin-susceptible Staphylococcus aureus, Staphylococcus epidermidis, Enterococcus spp., Candida albicans, Candida glabrata and Candida tropicalis was investigated by disc diffusion method. Results: It was observed that the extract prepared with ethanol showed the widest zone diameter against Enterococcus spp. (8mm) and S. epidermidis (8mm), the extracts with formaldehyde and ethyl acetate against E. coli (16mm, 28mm), the extract with acetone against Enterococcus spp. (21mm), and the extract with methanol against methicillin-susceptible S. aureus (28mm). For the yeasts, the extract prepared with ethanol showed the widest zone diameter against C. glabrata (12mm), the extract with formaldehyde against C. albicans (28mm) and C. tropicalis (28mm), and the extracts prepared with acetone, ethyl acetate, and methanol against C. albicans (19mm, 30mm, 22mm). Discussion and Conclusion: It was determined that the extract prepared with ethyl acetate showed wider zone diameter against the strains studied, compared to extracts prepared with the other solvents. Given that the widest zone diameters were observed against the strains E. coli, S. aureus and C. albicans, we think that it is important to conduct in vivo antimicrobial studies on the inhibitory effects of the Istranca oak indigenous to our country.

Özet

Amaç: Bu çalışmada, Istranca meşesi (Quercus hartwissiana Steven) kabuğu ekstraktlarının bakteriyelve fungal patojenlere karşı antimikrobiyal aktivitesini araştırmak amaçlanmıştır.Gereç ve Yöntemler: Istranca meşesinin, etanol, formaldehit, aseton, etil asetat ve metanol çözücüleriile ekstraksiyonu gerçekleştirilmiştir. Bu ekstraktlardan 50 µl alınarak steril disklere emdirildiktensonra ekstraktlarda bulunan çözücülerin sonucu etkilememesi amacıyla diskler 24 saat steril ortamdakurutulmuştur. Disk difüzyon yöntemiyle Pseudomonas aeruginosa, Acinetobacter baumannii, Escherichiacoli, Klebsiella pneumoniae, Salmonella spp., metisilin dirençli Staphylococcus aureus, metisilinduyarlı Staphylococcus aureus, Staphylococcus epidermidis, Enterococcus spp., Candida albicans,Candida glabrata ve Candida tropicalis türlerine karşı antimikrobiyal aktivite araştırılmıştır.Bulgular: En geniş zon çapının etanolle hazırlanan ekstraktta Enterococcus spp. (8 mm) ve S. epidermidis(8 mm) suşlarına, formaldehit ve etil asetatla hazırlanan ekstraktlarda E. Coli suşuna (16 mm,28 mm), asetonla hazırlanan ekstraktta Enterococcus spp. suşuna (21 mm), metanolle hazırlananekstraktta ise metisilin duyarlı S. aureus suşuna (28 mm) karşı oluştuğu görülmüştür. Mayalarda iseen geniş zon çapının etanolle hazırlanan ekstraktta C. glabrata suşuna (12 mm), formaldehitle hazırlananekstraktta C. albicans (28 mm) ve C. tropicalis (28 mm) suşlarına, aseton, etil asetat ve metanollehazırlanan ekstraktlarda C. albicans suşuna (19 mm, 30 mm, 22 mm) karşı oluştuğu saptanmıştır.Tartışma ve Sonuç: Etil asetat ile hazırlanan ekstraktın diğer çözücülerle hazırlanan ekstraktlara kıyaslaçalışılan suşlara karşı daha geniş zon çapı oluşturduğu saptanmıştır. En geniş zon çapı E. coli, S. aureusve C. albicans türlerinde oluştuğundan, ülkemizde doğal olarak yetişen Istranca meşesinin inhibeedici etkilerine yönelik in vivo antimikrobiyal çalışmalar yapılmasının önemli olduğunu düşünmekteyiz.

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Cite This Article

ÇALIŞKAN, E., DÜLGER, G., AKKUŞ, A., KILINÇEL, Ö. (2022). Antimicrobial Activity of the Istranca Oak (Quercus hartwissiana Steven) against Nosocomial Pathogens. Anadolu Kliniği Tıp Bilimleri Dergisi, Cilt: 23 - Sayı: 3, 157–162. https://ajindex.com/articles/437729

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