SavedAdd your journal

Journals / Akademik Gıda / 2020 / Cilt: 18 - Sayı: 3

Effect of Scaling up on Bioetanol Production from Sugar Beet Molasses by Vitreoscilla Hemoglobin Expressing Escherichia coli Strains

Vitreoscilla Hemoglobini Eksprese Eden Escherichia coli Suşları ile Şeker Pancarı Melasından Biyoetanol Üretiminde Ölçek Büyütmenin Etkisi

Taner Şar
Meltem Yeşilçimen Akbaş
Pages
264–269
Publication Date
2022-10-11
DOI
—
🏛️Bibliographic metadata and full text access for this article are provided via DergiPark.
DergiPark Record ↗
✨

AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

It was shown that scaling up process had positive effect on bioethanol production from sugar beet molasses through VHb expressing strains.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:In the present work, bioethanol production through VHb expressing Escherichia coli TS3 and TS4 strains from sugarbeet molasses hydrolysate containing media was investigated.
📊
Key Findings & Data:The highest growth and ethanol production were obtained in MB2 (contains about 4% sugar) medium.
💡
Conclusion & Impact:It was shown that scaling up process had positive effect on bioethanol production from sugar beet molasses through VHb expressing strains.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

In the present work, bioethanol production through VHb expressing Escherichia coli TS3 and TS4 strains from sugarbeet molasses hydrolysate containing media was investigated. The highest growth and ethanol production were obtained in MB2 (contains about 4% sugar) medium. In MB2 medium, bioethanol production was enhanced by E. coli TS3 and TS4 strains from small scale to big scale fermentation. The highest ethanol productions by TS3 and TS4 strains were determined as 2.49% and 2.62% (v/v) respectively, with the largest volume of fermentation medium. It was shown that scaling up process had positive effect on bioethanol production from sugar beet molasses through VHb expressing strains.

Özet

Bu çalışmada, Vitreoscilla hemoglobini eksprese eden Escherichia coli TS3 ve TS4 suşlarının şeker pancarı melasıhidrolizatı ile hazırlanan besiyerleri (MB2-MB5) kullanılarak biyoetanol üretimleri incelenmiştir. Kullanılan farklı şekerkonsantrasyonlu besiyerleri içerisinde en fazla etanol üretimi MB2 besiyeri ortamında (yaklaşık %4 şeker içeren)gerçekleştirilmiştir. MB2 besiyerinde, küçük ölçekten büyük ölçeğe doğru biyoetanol üretiminin %10-17 oranındaarttığı saptanmıştır. En fazla biyoetanol üretim miktarları en büyük hacimdeki fermentasyon ortamında TS3 ve TS4suşları ile sırasıyla %2.49 ve %2.62 (v/v) olarak belirlenmiştir. Yapılan bu çalışmada ölçek büyütmenin VHbekspresyonu yapan bakterilerle şeker pancarı melasından etanol üretimini olumlu etkilediği belirlenmiştir.

🔖

Cite This Article

Şar, T., Akbaş, M. Y. (2022). Effect of Scaling up on Bioetanol Production from Sugar Beet Molasses by Vitreoscilla Hemoglobin Expressing Escherichia coli Strains. Akademik Gıda, Cilt: 18 - Sayı: 3, 264–269. https://ajindex.com/articles/432476

📄 Full Text PDF Viewer

📄

PDF görüntüleyici hazırlanıyor...

References (14)

  1. Demirbas, A. (2007). Progress and recent trends in biofuels. Progress in Energy and Combustion Science, 33(1), 1-18
  2. U.S Department of Energy (US DOE). (2016). Global Ethanol Production by Country/Region, Renewable Fuels Association, Washington, USA
  3. RFA. (2019). Renewable Fuels Association, Washington, USA
  4. Regassa, T.H., Wortmann, C.S. (2014). Sweet sorghum as a bioenergy crop: literature review. Biomass and Bioenergy, 64, 348-355
  5. Arimi, M.M., Zhang, Y., Götz, G., Kiriamiti, K., Geißen, S.U. (2014). Antimicrobial colorants in molasses distillery wastewater and their removal technologies. International Biodeterioration & Biodegradation, 87, 34-43
  6. Broughton, N.W., Dalton, C.C., Jones, G.C., Williams, E.L. (1995). Adding value to sugar beet pulp. International Sugar Journal (United Kingdom)
  7. Kühnel, S., Schols, H.A., Gruppen, H. (2011). Aiming for the complete utilization of sugar-beet pulp: examination of the effects of mild acid and hydrothermal pretreatment followed by enzymatic digestion. Biotechnology for Biofuels, 4(1), 1-14
  8. Fukuda, H., Kondo, A., Tamalampudi, S. (2009). Bioenergy: Sustainable fuels from biomass by yeast and fungal whole-cell biocatalysts. Biochemical Engineering Journal, 44(1), 2-12
  9. Ozdingis, A.G.B., Kocar, G. (2018). Current and future aspects of bioethanol production and utilization in Turkey. Renewable and Sustainable Energy Reviews, 81, 2196-2203
  10. Pattanakittivorakul, S., Lertwattanasakul, N., Yamada, M., Limtong, S. (2019). Selection of thermotolerant Saccharomyces cerevisiae for high temperature ethanol production from molasses and increasing ethanol production by strain improvement. Antonie van Leeuwenhoek, 1-16
  11. Veana, F., Martínez-Hernández, J.L., Aguilar, C.N., Rodríguez-Herrera, R., Michelena, G. (2014). Utilization of molasses and sugar cane bagasse for production of fungal invertase in solid state fermentation using Aspergillus niger GH1. Brazilian Journal of Microbiology, 45(2), 373-377
  12. Suksawang, S., Cheirsilp, B., Yeesang, J. (2016). Production of kefiran from molasses and spent yeast cells by Lactobacillus kefiranofaciens JCM 6985. Asia-Pacific Journal of Science and Technology, 21(2), 59-67
  13. Akbas, M.Y., Sar, T., Ozcelik, B. (2014). Improved ethanol production from cheese whey, whey powder, and sugar beet molasses by “Vitreoscilla hemoglobin expressing” Escherichia coli. Bioscience Biotechnology Biochemistry, 78(4), 687-694
  14. Taskin, M., Ortucu, S., Ay