Journals / Afyon Kocatepe Üniversitesi Fen ve Mühendislik Bilimleri Dergisi / 2019 / Cilt: 19 - Sayı: 1

The effect of different environmental conditions on exopolysaccharide production of Micrococcus sp.

Farklı Ortam Koşullarının Micrococcus sp. Ekzopolisakkarit Üretimine Etkisi

Pages
40–46
Publication Date
2022-10-12
DOI
—
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

At the end of the trials, the maximum EPS production was found at pH 6, in media including 100 mg/l Remazol Blue at 40 °C and at the end of 48 h incubation period as 257.2 mg/l.

📌 3 Key Takeaways

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Objective & Hypothesis:The effect of different pH values, temperature degrees, Remazol Blue concentrations and incubation periods on exopolysaccharide (EPS) production of the bacteria was investigated in this study which was performed with Micrococcussp. isolated from industrial wastewater.
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Key Findings & Data:In trials at 30 C including Remazol Blue with different pH (pH 6-9), the production of high amount of EPS was found at pH 6.
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Conclusion & Impact:At the end of the trials, the maximum EPS production was found at pH 6, in media including 100 mg/l Remazol Blue at 40 °C and at the end of 48 h incubation period as 257.2 mg/l.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

The effect of different pH values, temperature degrees, Remazol Blue concentrations and incubation periods on exopolysaccharide (EPS) production of the bacteria was investigated in this study which was performed with Micrococcussp. isolated from industrial wastewater. In trials at 30 C including Remazol Blue with different pH (pH 6-9), the production of high amount of EPS was found at pH 6. At initial Remazol Blue concentrations between 100-400 mg/l (pH 6, 30 C), the bacteria was produced the maximum EPS production at 200 mg/l Remazol Blue as 234.8 mg/l. In trials to investigate the effect of increasing temperature degrees on EPS amount produced by Micrococcus sp., it is observed that the maximum EPS production was reached at 40 C. When the effect of incubation period on production of EPS was investigated, increasing incubation period was decreased EPS production, the highest EPS production was determined at the end of 48 h incubation period in which biomass production has just begun. At the end of the trials, the maximum EPS production was found at pH 6, in media including 100 mg/l Remazol Blue at 40 °C and at the end of 48 h incubation period as 257.2 mg/l.

Özet

Endüstriyel atık sulardan izole edilen Micrococcus sp. ile yapılan çalışmada, farklı pH değerlerinin,Remazol Blue konsantrasyonlarının, sıcaklık derecelerinin ve inkübasyon sürelerinin bakterinin ürettiğiekzopolisakkarit (EPS) miktarına etkisi araştırılmıştır. Remazol Blue bulunan ortamlarda farklı pH’larda(pH 6-9) 30 C de gerçekleştirilen deneylerde, pH 6’da yüksek miktarda EPS üretildiği belirlenmiştir.Bakteri 100-400 mg/l arasındaki başlangıç Remazol Blue konsantrasyonlarında (pH 6, 30 C) en iyi EPSüretimini, 200 mg/l Remazol Blue konsantrasyonunda 234.8 mg/l olarak yapmıştır. Artan sıcaklıkderecelerinin Micrococcus sp. tarafından üretilen EPS miktarlarına etkisinin araştırıldığı denemelerdeise en yüksek EPS üretimine 40 °C’de ulaşıldığı görülmüştür. İnkübasyon süresinin üretilen EPS miktarınaetkisi araştırıldığında, artan inkübasyon süresinin EPS üretimini azalttığı, en yüksek EPS üretiminebiyokütle üretiminin yeni başladığı 48 saatlik inkübasyon süresi sonunda ulaşıldığı belirlenmiştir.Denemeler sonunda en yüksek EPS üretimi, pH 6’ da, 100 mg/l Remazol Blue içeren ortamda, 40 C’deve 48 saat inkübasyon süresi sonrasında 257.2 mg/l olarak elde edilmiştir.

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Cite This Article

KILIÇ, N. K., DÖNMEZ, G. (2022). The effect of different environmental conditions on exopolysaccharide production of Micrococcus sp.. Afyon Kocatepe Üniversitesi Fen ve Mühendislik Bilimleri Dergisi, Cilt: 19 - Sayı: 1, 40–46. https://ajindex.com/articles/430130

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