Organize Sanayi Bölgesi (Uşak) Atıklarından Kurşun (Pb) Biyobirikimi Yapabilen Bakterilerin İzolasyonu ve İdentifikasyonu

Objective & Hypothesis:In this study, 16 bacterial isolates were obtained by using pour plate method from ceramic factory waste samples located in Uşak province.

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Key Findings & Data:The identification of 6 bacteria capable of lead (Pb) accumulation was performed. 16S rRNA sequence analysis of the isolates were performed and bacteria capable of heavy metal accumulation were detected; SAB2: %99 Enterobacter cancerogenus; Pantoea agglomerans; Enterobacter cloacae, SÇB9: %100 Bacillus mycoides; Bacillus cereus; Bacillus weihenstephanensis, SAB4: %99 Acinetobacter haemolyticus, SAB6: %100 Pseudomonas aeruginosa, SAB7:%100 Bacillus subtilis; Bacillus halotolerans; Bacillus mojavensis, SAB9: %100 Bacillus aryabhattai, Bacillus meqaterium.

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Conclusion & Impact:FTIR analysis results show that amine groups are effective in biosorption.

🔬Design:Research Article

📍Scope:Comprehensive Sample

⚙️Variables:Domain Parameters

Abstract

In this study, 16 bacterial isolates were obtained by using pour plate method from ceramic factory waste samples located in Uşak province. The identification of 6 bacteria capable of lead (Pb) accumulation was performed. 16S rRNA sequence analysis of the isolates were performed and bacteria capable of heavy metal accumulation were detected; SAB2: %99 Enterobacter cancerogenus; Pantoea agglomerans; Enterobacter cloacae, SÇB9: %100 Bacillus mycoides; Bacillus cereus; Bacillus weihenstephanensis, SAB4: %99 Acinetobacter haemolyticus, SAB6: %100 Pseudomonas aeruginosa, SAB7:%100 Bacillus subtilis; Bacillus halotolerans; Bacillus mojavensis, SAB9: %100 Bacillus aryabhattai, Bacillus meqaterium. According to ICP analysis results, for the first time, Bacillus mycoides (57%) and Bacillus aryabhattai (46%) were found to be capable of lead biosorption. FTIR analysis results show that amine groups are effective in biosorption.

Özet

Bu çalışmada Uşak ili sınırları içinde bulunan Seramik fabrikası atık örneklerinden dökme plak yöntemikullanılarak 16 bakteri izolatı elde edildi ve kurşun (Pb) birikimi yapabilen 6 bakterinin identifikasyonuyapıldı. İzolatların 16S rRNA sekans analizi yapıldı ve ağır metal birikimi yapabilen bakteriler tespit edildi;SAB2: %99 Enterobacter cancerogenus; Pantoea agglomerans; Enterobacter cloacae, SÇB9: %100Bacillus mycoides; Bacillus cereus; Bacillus weihenstephanensis, SAB4: %99 Acinetobacterhaemolyticus, SAB6: %100 Pseudomonas aeruginosa, SAB7:%100 Bacillus subtilis; Bacillus halotolerans;Bacillus mojavensis, SAB9: %100 Bacillus aryabhattai; Bacillus meqaterium. ICP analiz sonuçlarına göreBacillus mycoides (% 57) ve Bacillus aryabhattai'nin (% 46) kurşun biyosorbsiyonu yapabildiği, ilk kez buçalışma ile tespit edildi. FTIR analiz sonuçları, amin gruplarının biyosorbsiyonda etkin olduğunugöstermektedir.

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Cite This Article

AYDIN, B., KORCAN, S. E., ÇETİN, G. K., ALPASLAN, N., AKKUŞ, G. U., AŞÇI, F. (2022). Organize Sanayi Bölgesi (Uşak) Atıklarından Kurşun (Pb) Biyobirikimi Yapabilen Bakterilerin İzolasyonu ve İdentifikasyonu. Afyon Kocatepe Üniversitesi Fen ve Mühendislik Bilimleri Dergisi, Cilt: 19 - Sayı: 3, 490–507. https://ajindex.com/articles/429916

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