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Journals / Afyon Kocatepe Üniversitesi Fen ve Mühendislik Bilimleri Dergisi / 2019 / Cilt: 19 - Sayı: 1

Cytotoxic and Genotoxic Effects of Primite 20 WP Containing 20% Pyridaben on Allium cepa Root Meristematic Cells

%20 Pyridaben İçeren Primite 20 WP’nin Allium cepa Kök Meristematik Hücrelerine Üzerine Sitotoksik ve Genotoksik Etkileri

Recep LİMAN
Pages
5–14
Publication Date
2022-10-12
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

PCPshould be used carefully and investigated its cyto-genotoxic effects with other toxicology test systems.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:Primite 20 WP containing 20% pyridaben (PCP) is used as an acaricide and insecticide.
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Key Findings & Data:The amount of PCP concentration that reduces the growth of root tips by 50% compared to the negative control group (EC50)value was determined as 50 ppm by Allium root growth inhibition test.
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Conclusion & Impact:PCPshould be used carefully and investigated its cyto-genotoxic effects with other toxicology test systems.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Primite 20 WP containing 20% pyridaben (PCP) is used as an acaricide and insecticide. In this study, cytotoxic and genotoxic effects of PCP were investigated on the root meristem cells of Allium cepa for its effects on root growth, mitotic index (MI), mitotic phases, chromosomal abnormalities (CAs) and DNA damage by using Allium ana-telophase and Comet assays. The amount of PCP concentration that reduces the growth of root tips by 50% compared to the negative control group (EC50)value was determined as 50 ppm by Allium root growth inhibition test. A. cepa roots were treated with three concentrations of PCP (25, 50 and 100 ppm), distilled water (negative control) and methyl methane sulphonate (MMS, 10 ppm, positive control) for 24, 48, 72 and 96 h. PCPshowed a cytotoxic effect by reducing root growth and MI, but also showed genotoxic effect by increasing CAs (disturbed anatelophase, chromosome laggards, stickiness, bridges and polyploidy) and DNA damage at significant levels. PCPshould be used carefully and investigated its cyto-genotoxic effects with other toxicology test systems.

Özet

%20 Pyridaben içeren Primite 20 WP (PİP) akarisit ve insektisit olarak kullanılmaktadır. Bu çalışmadaPİP’in sitotoksik ve genotoksik etkileri Allium cepa kök meristematik hücrelerinde kök büyümesi, mitotikindeks (Mİ), kromozomal anormallik (KA)’ler ve DNA hasarı üzerindeki etkileri Allium ana-telofaz veKomet testleri kullanılarak araştırılmıştır. Allium kök büyüme engelleme testi ile PİP’in kök uçlarınınbüyümesini negatif kontrol grubuna göre %50 oranında düşüren konsantrasyon (EC50) değeri, 50 ppmolarak bulunmuştur. A. cepa kökleri PİP’in üç farklı konsantrasyonu (25, 50 ve 100 ppm), distile su(negatif kontrol) ve metil metan sülfonata (MMS, 10 ppm, pozitif kontrol) 24, 48, 72 ve 96 saat maruzbırakılmıştır. PİP; kök büyümesini ve Mİ’yi istatistiksel olarak azaltarak sitotoksik etki, KA’ları (bozulmuşana-telofaz, kalgın kromozom, yapışıklık, köprü ve poliploidi) ve DNA hasarını istatistitiksel olarakartırarak genotoksik etki göstermiştir. PİP kullanımına dikkat edilmeli ve sito-genotoksik etkileri diğertoksisite test sistemleri ile araştırılmalıdır.

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Cite This Article

LİMAN, R. (2022). Cytotoxic and Genotoxic Effects of Primite 20 WP Containing 20% Pyridaben on Allium cepa Root Meristematic Cells. Afyon Kocatepe Üniversitesi Fen ve Mühendislik Bilimleri Dergisi, Cilt: 19 - Sayı: 1, 5–14. https://ajindex.com/articles/429836

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References (7)

  1. Bloom, S.E., Lemley, A.T. and Muscarella, D.E., 2005. Potentiation of apoptosis by heat stress plus pesticide exposure in stress resistant human Blymphoma cells and its attenuation through interaction with follicular dendritic cells: role for cJun N-terminal kinase signaling. Toxicological Sciences, 89, 214-223.
  2. Bose, A. and Beal, M.F., 2016. Mitochondrial dysfunction in Parkinson's disease. Journal of Neurochemistry. 139, 216-231. Caritá, R. and Marin-Morales, M.A., 2008. Induction of chromosome aberrations in the Allium cepa test system caused by the exposure of seeds to industrial effluents contaminated with azo dyes Chemospher,. 72, 722-725.
  3. Chaparro, T.R., Botta C.M. and Pires, E.C., 2010. Biodegradability and toxicity assessment of bleach plant effluents treated anaerobically. Water Science and Technology, 62, 1312-1319.
  4. Charli, A., Jin, H., Anantharam, V., Kanthasamy, A. and Kanthasamy, A.G., 2016. Alterations in mitochondrial dynamics induced by tebufenpyrad and pyridaben in a dopaminergic neuronal cell culture model. Neurotoxicology, 53, 302-313.
  5. Chen, Q., Vazques, E.J., Moghaddas, S., Hoppel, C.L. and Lesnefsky, E.J., 2003. Production of reactive oxygen species by mitochondria: Central role of complex III. Journal of Biological Chemistry, 278, 36027– 36031. Dhawan, A., and Anderson, D., 2016.The comet assay in toxicology (Vol. 30). Second Edition. Royal Society of Chemistry. 3-64.
  6. Dönbak, D.B., 2017. İnsan periferal lenfositlerinde pyridaben insektisitinin ka, kkd ve mn testleri ile genotoksisitesinin değerlendirilmesi, Yüksek Lisans Tezi, Kahramanmaraş Sütçü İmam Üniversitesi, Fen Bilimleri Enstitüsü. Kahramanmaraş. 88. El-Ghamery, A.A. and Mousa, M.A., 2017. Investigation on the effect of benzyladenine on the germination, radicle growth and meristematic cells of Nigella sativa L. and Allium cepa L. Annals of Agricultural Sciences, 62, 11-21.
  7. El-Ghamery, A.A., El-Nahas, A.I. and Mansour, M.M., 2000. The action of atrazine herbicide as an indicator of cell division on chromosomes and nucleic acid content in root meristems of Allium cepaand Vicia faba. Cytologia, 65, 277–287. European Food Safety Authority (EFSA)., 2010. Conclusion on the peer review of the pesticide risk assessment of the active substance pyridaben. EFSA Journal, 8, 1632. Evseeva, T.I. Geras’kin, S.A., Shuktomova, I.I. and Taskaev, A.I., 2005. Genotoxicity and cytotoxicity assay of water sampled from the underground nuclear explosion site in the north of the Perm region (Russia),