Journals / Adli Tıp Dergisi / 2014 / Cilt: 28 - Sayı: 1

FARKLI ADLİ BİYOLOJİK KALINTILARDAN DNA İZOLASYONU İÇİN KALSİYUM TARTARAT JELİNDE ADSORPSİYON KROMATOGRAFİSİ VE TOYOPEARL HW-65F MİKROKOLONLU JEL-FİLTRASYON KROMATOGRAFİSİ KOMBİNASYONUNA DAYALI YÜKSEK ETKİLİ EVRENSEL BİR YÖNTEM

Highly effective universal method for DNA isolation from different forensic biological traces based on the combination of adsorption chromatography on calcium-tartrate gel and gel-filtration chromatography with Toyopearl HW-65F microcolumns.

Pages
24–32
Publication Date
2022-10-11
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Highly pure concentrated DNA free of contaminants such as hematoporphyrins, chloroporphyrins, melanins, humic acids, biopolymers and products of their degradation including primer-like products can be obtained with our metho

📌 3 Key Takeaways

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Objective & Hypothesis:AMAÇ: A universal method for DNA isolation and purification from the wide spectrum of biological traces has been developed.
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Key Findings & Data:The method is based on the combination of two consequently performed methods - adsorption chromatography on calcium-tartrate gel and gel-filtration chromatography with Toyopearl HW-65F microcolumns.
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Conclusion & Impact:Highly pure concentrated DNA free of contaminants such as hematoporphyrins, chloroporphyrins, melanins, humic acids, biopolymers and products of their degradation including primer-like products can be obtained with our metho
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

AMAÇ: A universal method for DNA isolation and purification from the wide spectrum of biological traces has been developed. The method is based on the combination of two consequently performed methods - adsorption chromatography on calcium-tartrate gel and gel-filtration chromatography with Toyopearl HW-65F microcolumns. Highly pure concentrated DNA free of contaminants such as hematoporphyrins, chloroporphyrins, melanins, humic acids, biopolymers and products of their degradation including primer-like products can be obtained with our metho

Özet

Geniş ölçekli biyolojik kalıntı- lardan DNA izolasyonu ve saf- laştırılması ile ilgili evrensel birmetod geliştirilmiştir. Bu metotard arda uygulanan iki meto- dun (Kalsiyum tartarat jelindeadsorpsiyon kromatografisi veToyopearl HW-65F mikrokolon- lu jel-filtrasyon kromatografisi)kombinasyonuna dayanmaktadır.Hematoporfirinler, kloroporfirin- ler, melaninler, humik asit, biyo- polimerler ve bunların degradas- yonuyla oluşan primer-benzeriürünler gibi kontaminantlardanayrıştırılmış yüksek saflıkta vekonsantrasyonda DNA, bu me- todla elde edilebilir.

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Cite This Article

KOTOVA, S., TYSBOVSKY, I., SHYLA, A., KUZUB, N. (2022). FARKLI ADLİ BİYOLOJİK KALINTILARDAN DNA İZOLASYONU İÇİN KALSİYUM TARTARAT JELİNDE ADSORPSİYON KROMATOGRAFİSİ VE TOYOPEARL HW-65F MİKROKOLONLU JEL-FİLTRASYON KROMATOGRAFİSİ KOMBİNASYONUNA DAYALI YÜKSEK ETKİLİ EVRENSEL BİR YÖNTEM. Adli Tıp Dergisi, Cilt: 28 - Sayı: 1, 24–32. https://ajindex.com/articles/428768

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References (4)

  1. Graham DE. The isolation of high molecular weight DNA from whole organisms or large tissue masses. Anal Biochem 1978;85(2):609– 13
  2. Akhrem A, Drozhdenyuk AP. Calcium tartrate gel. Anal Biochem 1989;179(1):86-9
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