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Journals / Adıyaman Üniversitesi Fen Bilimleri Dergisi / 2018 / Cilt: 8 - Sayı: 2

The Effect on Antioxidant Defense System of Chondroitin-4-Sulphate in Human Erythrocytes Subject to High Glucose Levels: In The Laboratory Conditions

Laboratuvar Koşullarında Yüksek Glukoza Maruz Kalmış İnsan Eritrositlerinde Kondroitin-4-Sülfatın Antioksidan Savunma Sistemi Üzerine Etkisi

Seda BALKAN
Pages
98–114
Publication Date
2022-10-12
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

As a result of the statistical evaluations, the significant increases(p

📌 3 Key Takeaways

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Objective & Hypothesis:The purpose of this research was to evaluation the effects on antioxidant defensesystem of chondroitin-4-sulphate (C4S) in high glucose treated human erythrocytes. 10-20 ml venous blood samples from ten healthy volunteers ages 20-40, nonsmoker, wereaccumulated in ethylenediamine tetraacetic acid (EDTA) vials for the preparation of redblood cell (RBC) suspensions.
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Key Findings & Data:Blood for in vitro treatments was divided into six groups.The activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase(GSH-Px), acetylcholinesterase (AChE), the levels of glutathione (GSH) andmalondialdehyde (MDA) were calculated to determine the effects on antioxidant defensesystem in all groups.
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Conclusion & Impact:As a result of the statistical evaluations, the significant increases(p
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

The purpose of this research was to evaluation the effects on antioxidant defensesystem of chondroitin-4-sulphate (C4S) in high glucose treated human erythrocytes. 10-20 ml venous blood samples from ten healthy volunteers ages 20-40, nonsmoker, wereaccumulated in ethylenediamine tetraacetic acid (EDTA) vials for the preparation of redblood cell (RBC) suspensions. Blood for in vitro treatments was divided into six groups.The activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase(GSH-Px), acetylcholinesterase (AChE), the levels of glutathione (GSH) andmalondialdehyde (MDA) were calculated to determine the effects on antioxidant defensesystem in all groups. As a result of the statistical evaluations, the significant increases(p

Özet

Bu çalışmada, yüksek glukoza maruz kalmış insan eritrositlerinde kondroitin-4- sülfatın (C4S) antioksidan savunma sistemine olan etkisi araştırılmıştır. Eritrosit süspansiyonu hazırlamak için, sigara içmeyen, 20-40 yaş aralığında sağlıklı gönüllülerden 10-20 ml venöz kanı etilendiamin tetraasetik asitli (EDTA) tüplere toplanmıştır. İn vitro uygulamalar için kanlar 6 gruba ayrılmıştır. Tüm gruplarda antioksidan savunma sistemi üzerine olan etkilerin belirlenmesi için süperoksit dismutaz (SOD), katalaz (CAT), glutatyon peroksidaz (GSH-Px), asetilkolinesteraz (AChE) enzim aktiviteleri, glutatyon (GSH) ve MDA düzeyleri ölçülmüştür. İstatistiksel değerlendirmeler sonucunda, C4S uygulanan gruplar ile sadece glukoz uygulanan gruplar karşılaştırıldığında GSH-Px ve AChE enzim aktivitelerinde önemli ölçüde artış (p

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Cite This Article

BALKAN, S. (2022). The Effect on Antioxidant Defense System of Chondroitin-4-Sulphate in Human Erythrocytes Subject to High Glucose Levels: In The Laboratory Conditions. Adıyaman Üniversitesi Fen Bilimleri Dergisi, Cilt: 8 - Sayı: 2, 98–114. https://ajindex.com/articles/428505

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