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Journals / Adıyaman Üniversitesi Fen Bilimleri Dergisi / 2019 / Cilt: 9 - Sayı: 2

The in vitro Effect of Neonicotinoid Insecticides Imidacloprid and Thiamethoxam on Glutathione Reductase Enzyme Activity from Baker's Yeast (Saccharomyces cerevisiae)

Neonikotinoid İnsektisitler İmidakloprid ve Tiyamethoksam’ın Ekmek Mayası (Saccharomyces cerevisiae) Glutatyon Redüktaz Enzim Aktivitesi Üzerine in vitro Etkisi

Hasan KARADAĞ
Pages
266–273
Publication Date
2022-10-12
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

The present study showed that the tested neonicotinoid insecticides did not cause significant changes in GR enzyme activities.

📌 3 Key Takeaways

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Objective & Hypothesis:In this study, glutathione reductase from baker's yeast (Saccharomyces cerevisiae) (GR) exposed to 0, 25, 50, 100, 250 and 500 mg/L neonicotinoid insecticides imidacloprid (IMI) and thiamethoxam (TMX).
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Key Findings & Data:Under the exposure of 25, 50, 100, 250 and 500 mg/L concentrations, % GR activity alterations were calculated as -1.01; +0.50; 0.00; 0.00 and -1.01 in IMI applications, while these alterations were calculated as -2.91; -1.46; -0.97; -1.46 and -0.97 in TMX applications, respectively.
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Conclusion & Impact:The present study showed that the tested neonicotinoid insecticides did not cause significant changes in GR enzyme activities.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

In this study, glutathione reductase from baker's yeast (Saccharomyces cerevisiae) (GR) exposed to 0, 25, 50, 100, 250 and 500 mg/L neonicotinoid insecticides imidacloprid (IMI) and thiamethoxam (TMX). Under the exposure of 25, 50, 100, 250 and 500 mg/L concentrations, % GR activity alterations were calculated as -1.01; +0.50; 0.00; 0.00 and -1.01 in IMI applications, while these alterations were calculated as -2.91; -1.46; -0.97; -1.46 and -0.97 in TMX applications, respectively. According to control activity, no statistical changes were observed in GR activities (p>0.05). The present study showed that the tested neonicotinoid insecticides did not cause significant changes in GR enzyme activities.

Özet

Bu çalışmada, ekmek mayası (Saccharomyces cerevisiae) glutatyon redüktazı (GR), 0, 25, 50, 100, 250 ve 500 mg/L neonikotinoid insektisit imidakloprid (IMI) ve tiyamethoksam’a (TMX) maruz bırakılmıştır. 25, 50, 100, 250 ve 500 mg/L konsantrasyonlara maruz bırakılma ile, % GR aktivitesindeki değişimler, IMI uygulamalarında sırasıyla; -1.01; +0.50; 0.00; 0.00 ve -1.01 olarak hesaplanırken, TMX uygulamalarında sırasıyla -2.91; -1.46; -0.97; -1.46 ve -0.97 olarak hesaplanmıştır. Kontrol aktivitesine göre GR aktivitelerinde istatistiksel bir değişiklik gözlemlenmemiştir (p>0.05). Bu çalışma, test edilen neonikotinoid insektisitlerin GR enzim aktivitelerinde önemli değişikliklere neden olmadığını göstermiştir.

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Cite This Article

KARADAĞ, H. (2022). The in vitro Effect of Neonicotinoid Insecticides Imidacloprid and Thiamethoxam on Glutathione Reductase Enzyme Activity from Baker's Yeast (Saccharomyces cerevisiae). Adıyaman Üniversitesi Fen Bilimleri Dergisi, Cilt: 9 - Sayı: 2, 266–273. https://ajindex.com/articles/428494

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