Journals / Adıyaman Üniversitesi Fen Bilimleri Dergisi / 2019 / Cilt: 9 - Sayı: 1

A Time-Course Study on Essential Oil of Rosemary (Rosmarinus officinalis) Under Drought Stress

Kuraklık Stresi Altındaki Biberiyenin (Rosmarinus officinalis) Uçucu Yağ Bileşenlerinin Zamana Bağlı Olarak Değişimi Üzerine Bir Çalışma

Pages
165–189
Publication Date
2022-10-12
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Drought and well-watered(control) groups were clearly discriminated and confirmed using differential statisticaltools, suggesting the plausible role of metabolites in response to the changingenvironmental conditions.

📌 3 Key Takeaways

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Objective & Hypothesis:Along with the present study, the changes in essential oil profile of rosemary(Rosmarinus officinalis) under drought stress were investigated.
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Key Findings & Data:Accordingly, 26 compounds were identified using gaschromatographycoupled with headspace system.
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Conclusion & Impact:Drought and well-watered(control) groups were clearly discriminated and confirmed using differential statisticaltools, suggesting the plausible role of metabolites in response to the changingenvironmental conditions.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Along with the present study, the changes in essential oil profile of rosemary(Rosmarinus officinalis) under drought stress were investigated. The leaf samples ofrosemary were collected on three consecutive days and then the drought stressed groupswere irrigated as recovery stage. Accordingly, 26 compounds were identified using gaschromatographycoupled with headspace system. Of the compounds, α-pinene,camphene, β-pinene, β-myrcene, p-cymene, D-limonene, eucalyptol, and camphor areof the major compounds, representing the 84.874 % of the identified compounds. Ofthose compounds, α-pinene, β-myrcene, and camphor percentage increased with thedrought but the percentage of β-pinene decreased. Moreover, the changes in lipid, amideand carbohydrate regions for the samples were examined using Attenuated TotalReflectance Fourier Transform Infrared spectroscopy. The intensities: 2920 to 2852,1727 to 1687 and 1452 to 1035 cm-1bands corresponding to the lipids, amides, andcarbohydrates, respectively were higher in CRD1, CRD2, CRD3, CD3, SD3, SRD1.Considered all experimental groups, the intensities were partially higher in controlgroup. For the discrimination of the experimental groups, variance analysis, clusteringanalysis, and principal component analysis were performed. Drought and well-watered(control) groups were clearly discriminated and confirmed using differential statisticaltools, suggesting the plausible role of metabolites in response to the changingenvironmental conditions.

Özet

Bu çalışma ile birlikte, kuraklık stresi altındaki biberiyenin (Rosmarinus officinalis) uçucu yağ profilinde meydana gelen değişimler araştırılmıştır. Biberiye yaprağı örnekleri üç ardışık gün içerisinde toplanmış ve daha sonra kuraklık stresine maruz bırakılan bitkiler iyileştirme amaçlı sulanmıştır. Sonuç olarak GC-MS Headspace sistemi ile yapılan analize göre 26 bileşen belirlenmiştir. a-pinen, kamfen, β-pinen, βmyrcene, p-cymene, D-limonen, okaliptol ve kafur, tanımlanan bileşiklerin % 84, 874'ünü temsil eden ana bileşenler olarak belirlenmiştir. Bu bileşiklerin a-pinen, βmyrcene ve kafur yüzdesi kuraklıkla artmış, ancak β-pinen yüzdesi azalmıştır. Ayrıca, Zayıflatılmış Toplam Yansıma Fourier Dönüşümü Kızılötesi spektroskopisi kullanılarak biberiye yapraklarındaki lipit, amit ve karbonhidrat bölgelerinde meydana gelen değişimler de incelenmiştir. Lipitlere, amitlere ve karbonhidratlara karşılık gelen 2920 ila 2852, 1727 ila 1687 ve 1452 ila 1035 cm-1 bant yoğunluklarının sırasıyla CRD1, CRD2, CRD3, CD3, SD3, SRD1'de daha yüksek olduğu belirlenmiştir. Tüm deney grupları dikkate alındığında, kontrol grubunda lipit, amit ve karbonhidrat bant yoğunluklarının kısmen daha yüksek olduğu gözlenmiştir. Deney gruplarının ayrımı için varyans analizi, kümeleme analizi ve temel bileşen analizi yapılmıştır. Kuraklık ve sulanan (kontrol grubu) gruplar, ayırt edici istatistiksel araçlar kullanılarak doğru bir şekilde ayırt edilmiş ve doğrulanmıştır. Ayrıca değişen çevresel koşullara yanıt olarak metabolitlerin olası rolü ortaya konulmuştur.

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Cite This Article

KULAK, M. (2022). A Time-Course Study on Essential Oil of Rosemary (Rosmarinus officinalis) Under Drought Stress. Adıyaman Üniversitesi Fen Bilimleri Dergisi, Cilt: 9 - Sayı: 1, 165–189. https://ajindex.com/articles/428483

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References (10)

  1. Hamrouni, I., Salah, H. B., Marzouk, B., Effects of water-deficit on lipids of safflower aerial parts, Phytochem., 58, 277–80, 2001.
  2. Laribi, B., Bettaieb, I., Kouki, K., Sahli, A., Mougou, A., Marzouk, B., Water deficit effects on caraway (Carum carvi L.) growth, essential oil and fatty acid composition, Ind Crops Prod., 30, 372–379, 2009.
  3. Sangwan, N. S., Farooqi, A. H. A., Shabih, F., Sangwan, R.S., Regulation of essential oil production in plants, Plant Growth Regul., 34, 3–21, 2001. Kleinwächter, M., Paulsen, J., Bloem, E., Schnug, E., Selmar, D., Moderate drought and signal transducer induced biosynthesis of relevant secondary metabolites in thyme (Thymus vulgaris), greater celandine (Chelidonium majus) and parsley (Petroselinum crispum), Ind Crops Prod., 64, 158–166, 2015. Szabó, K., Radácsi, P., Rajhárt, P., Ladányi, M., Németh, É., Stress-induced changes of growth, yield and bioactive compounds in lemon balm cultivars, Plant Physiol Biochem., 119, 170–177, 2017.
  4. Laribi, B., Kouki, K., Sahli, A., Mougou, A., Marzouk, B., Essential oil and fatty acid composition of a Tunisian caraway (Carum carvi L.) seed ecotype cultivated under water deficit, Adv. Environ. Biol., 257–265, 2011.
  5. Bettaieb, I., Knioua, S., Hamrouni, I., Limam, F., Marzouk, B., Water-deficit impact on fatty acid and essential oil composition and antioxidant activities of cumin (Cuminum cyminum L.) aerial parts, J Agric Food Chem., 59, 328–334, 2011.
  6. Baghalian, K., Abdoshah, S., Khalighi-Sigaroodi, F., Paknejad, F., Physiological and phytochemical response to drought stress of German chamomile (Matricaria recutita L.), Plant Physiol Biochem., 49, 201–207, 2011.
  7. Khorasaninejad, S., Mousavi, A., Soltanloo, H., Hemmati, K., Khalighi, A., The effect of drought stress on growth parameters, essential oil yield and constituent of Peppermint (Mentha piperita L.). J. Med. Plants Res., 5, 5360–5365, 2011.
  8. Corell, M., Garcia, M. C., Contreras, J. I., Segura, M. L., Cermeño, P., Effect of Water Stress on Salvia officinalis L. Bioproductivity and Its Bioelement Concentrations, Commun Soil Sci Plant Anal., 43, 419–425, 2012.
  9. Bettaib-Rebey, I. B., Jabri-Karoui, I., Hamrouni-Sellami, I., Bourgou, S., Limam, F., Marzouk, B., Effect of drought on the biochemical composition and antioxidant activities of cumin (Cuminum cyminum L.) seeds, Ind Crops Prod., 36(1), 238–245, 2012.
  10. Farhoudi, R., Effect of drought stress on growth, physiology and flower essential oil yield of Iranian chamomile (Matricaria recutit