Toxic Effects of Glyphosate-Based Herbicide on Melanopsis praemorsa

Objective & Hypothesis:In the present study, we determined the 24, 48, 72, and 96 h LC50 values for a glyphosatebased commercial herbicide (GBH) for the aquatic snail Melanopsis praemorsa.

📊

Key Findings & Data:We examined subacute toxicity responses from M. praemorsa after 30-day exposure to 1/10, 1/5, and 1/2 of the 96 h LC50 value using several biochemical markers.

💡

Conclusion & Impact:These results showed that chronic GBH exposure inhibited GST, an important detoxifying enzyme.

🔬Design:Research Article

📍Scope:Comprehensive Sample

⚙️Variables:Domain Parameters

Abstract

In the present study, we determined the 24, 48, 72, and 96 h LC50 values for a glyphosatebased commercial herbicide (GBH) for the aquatic snail Melanopsis praemorsa. We examined subacute toxicity responses from M. praemorsa after 30-day exposure to 1/10, 1/5, and 1/2 of the 96 h LC50 value using several biochemical markers. Glutathione reductase (GR) and glutathione S-transferase (GST) activities were significantly inhibited when compared to control after 4 days of GBH exposure. On the twentieth day of exposure, GST and GR activities were inhibited in organisms exposed to both LC50/10 and LC50/5 compared to control. These results showed that chronic GBH exposure inhibited GST, an important detoxifying enzyme. GR, an important oxidative stress marker, was likely inhibited as a result of inhibition of the detoxification mechanism. Keywords: Melanopsis praemorsa; Biomarkers; Glyphosate-based herbicide.

Özet

Bu çalışmada, sucul salyangoz Melanopsis praemorsa için glifosat bazlı bir ticari herbisit (GBH)’in 24, 48, 72 ve 96 saat LC50 değerlerini belirledik. M. praemorsa'nın kronik toksisite tepkilerini ve 96 saat LC50 değerinin 1/10, 1/5 ve 1/2'sine 30 gün maruz kaldıktan sonra M. praemorsa'nın kronik toksisite cevaplarını birkaç biyokimyasal belirteç kullanarak inceledik. Glutatyon redüktaz (GR) ve glutatyon S-transferaz (GST) aktiviteleri, 4 günlük GBH maruziyetinden sonra kontrol ile karşılaştırıldığında istatistiksel olarak anlamlı şekilde inhibe oldu. Yirmi günlük maruz kalma sonucunda, GST ve GR aktiviteleri kontrole karşılaştırıldığında hem LC50/10'a hem de LC50/5'e maruz kalan organizmalarda inhibe olmuştur. Bu sonuçlar, kronik GBH maruziyetinin önemli bir detoksifiye edici enzim olan GST'yi inhibe ettiğini gösterdi. Önemli bir oksidatif stres belirteci olan GR, detoksifikasyon mekanizmasının inhibisyonu sonucu inhibe edildiği sonucuna varılabilinir. Anahtar Kelimeler: Melanopsis praemorsa; Biyobelirteçler; Glifosat bazlı herbisit.

🔖

Cite This Article

ÖZKAN, A. İ., ÇAKMAK, F., DEMİRCİ, Ö. (2022). Toxic Effects of Glyphosate-Based Herbicide on Melanopsis praemorsa. Adıyaman Üniversitesi Fen Bilimleri Dergisi, Cilt: 10 - Sayı: 1, 10–21. https://ajindex.com/articles/428453

📄 Full Text PDF Viewer

Open in New Tab ↗

↗New Tab

📄

PDF görüntüleyici hazırlanıyor...

References (2)

Schwarzenbach, R.P., Escher, B.I., Fenner, K., Hofstetter, T.B., Johnson, C.A., Von Gunten, U., Wehrli, B., The challenge of micropollutants in aquatic systems, Science, 313, 1072- 1077, 2006
Banaee, M., Sureda, A., Mirvaghefi, A., Ahmadi, K., Effects of diazinon on biochemical parameters of blood in rainbow trout (Oncorhynchus mykiss), Pesticide Biochemistry and Physiology, 99, 1-6, 2011. de Freitas Tallarico, L., Borrely, S.I., Hamada, N., Grazeffe, V.S., Ohlweiler, F.P., Okazaki, K., Granatelli, A.T., Pereira, I.W., de Bragança Pereira, C.A., Nakano, E., Developmental toxicity, acute toxicity and mutagenicity testing in freshwater snails Biomphalaria glabrata (Mollusca: Gastropoda) exposed to chromium and water samples, Ecotoxicology and Environmental Safety, 110, 208-215, 2014. Duke, S.O., Powles, S.B., Glyphosate: a once-in-a-century herbicide, Pest Management Science, 64, 319-325, 2008. Sayeed, I., Parvez, S., Pandey, S., Bin-Hafeez, B., Haque, R., Raisuddin, S., Oxidative stress biomarkers of exposure to deltamethrin in freshwater fish, Channa punctatus Bloch, Ecotoxicology and Environmental Safety, 56, 295-301, 2003. Folmar, L.C., Sanders, H., Julin, A., Toxicity of the herbicide glyphosate and several of its formulations to fish and aquatic invertebrates. Archives of Environmental Contamination and Toxicology, 8, 269-278, 1979. Lanctôt, C., Navarro-Martín, L., Robertson, C., Park, B., Jackman, P., Pauli, B.D., Trudeau, V.L., Effects of glyphosate-based herbicides on survival, development, growth and sex ratios of wood frog (Lithobates sylvaticus) tadpoles. II: agriculturally relevant exposures to Roundup WeatherMax® and Vision® under laboratory conditions, Aquatic Toxicology, 154, 291-303, 2014. Solomon, K., Thompson, D., Ecological risk assessment for aquatic organisms from over-water uses of glyphosate, Journal of Toxicology and Environmental, Health, Part B 6, 289- 324, 2003. Peruzzo, P.J., Porta, A.A., Ronco, A.E., Levels of glyphosate in surface waters, sediments and soils associated with direct sowing soybean cultivation in north pampasic region of Argentina, Environmental Pollution, 156, 61-66, 2008. Achiorno, C.L., de Villalobos, C., Ferrari, L., Validation test with embryonic and larval stages of Chordodes nobilii (Gordiida, Nematomorpha): Sensitivity to three reference toxicants, Chemosphere, 81, 133-140, 2010. İbrahim, D.M., Reduce, refine, replace: The failure of the three R's and the future of animal experimentation, The Univ