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Journals / Adıyaman Üniversitesi Fen Bilimleri Dergisi / 2021 / Cilt: 11 - Sayı: 1

Evaluating Effects of Black Carrot Extract on Testicular Carboxylesterase Activity and Oxidative Stress Parameters in Rats Exposed to Bisphenol A

Bisfenol A’ya Maruz Kalan Sıçanlarda Siyah Havuç Ekstresinin Testis Karboksilesteraz Aktivitesi ve Oksidatif Stres Parametreleri Üzerindeki Etkilerinin Değerlendirilmesi

Zafer ŞAHİN
Ahmet ÖZKAYA
Ertan YOLOĞLU
Miraç UÇKUN
Yunus ŞAHİN
Özgür BULMUŞ
Pages
73–86
Publication Date
2022-10-12
DOI
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

Our results showedBPA suppresses testicular detoxification activity and increases lipid peroxidation in rats.

📌 3 Key Takeaways

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Objective & Hypothesis:We aimed to evaluate the effects of black carrot extract on testicular carboxylesterase (Ces)activity, malondialdehyde (MDA), glutathione S-transferases (GST) and reduced glutathione(GSH) in male rats exposed to bisphenol A (BPA).
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Key Findings & Data:Adult Wistar albino male rats were dividedinto 4 groups as follows (n = 7/group): control, BPA, black carrot and BPA+Black carrot.Testicular Ces, MDA, GST and reduced GSH were analyzed by using a spectrophotometersystem.
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Conclusion & Impact:Our results showedBPA suppresses testicular detoxification activity and increases lipid peroxidation in rats.
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

We aimed to evaluate the effects of black carrot extract on testicular carboxylesterase (Ces)activity, malondialdehyde (MDA), glutathione S-transferases (GST) and reduced glutathione(GSH) in male rats exposed to bisphenol A (BPA). Adult Wistar albino male rats were dividedinto 4 groups as follows (n = 7/group): control, BPA, black carrot and BPA+Black carrot.Testicular Ces, MDA, GST and reduced GSH were analyzed by using a spectrophotometersystem. Testicular Ces activity was significantly lower only in the BPA group than the controlgroup (p < 0.001). Testicular MDA concentrations were higher only in the BPA group comparedwith the control group (p < 0.001). Reduced GSH level was higher in the black carrot andBPA+Black carrot groups than the control group (p < 0.05). GST activity was lower in the BPA group in comparison to the control group (p < 0.05). In the black carrot and BPA+Black carrotgroups, the GST activities were higher than the control group (p < 0.001). Our results showedBPA suppresses testicular detoxification activity and increases lipid peroxidation in rats. Weobserved that black carrot extract has a beneficial effect on the toxic and oxidative stress-relatedparameters caused by BPA exposure.

Özet

Bu çalışmada Bisfenol A'ya (BPA) maruz kalan erkek sıçanlarda siyah havuç ekstraktının testiküler karboksilesteraz (Ces) aktivitesi, malondialdehit (MDA) düzeyi, indirgenmiş glutatyon (GSH) ve glutatyon S-transferaz (GST) aktiviteleri üzerine etkilerini değerlendirmeyi amaçladık. Yetişkin erkek Wistar albino sıçanlar kontrol, BPA, siyah havuç ve BPA+Siyah havuç olmak üzere 4 gruba ayrıldı (n = 7/grup). Testis dokusunda Ces, MDA, indirgenmiş GSH ve GST düzeyleri mikroplaka okuyucu spektrofotometre sistemi kullanılarak analiz edildi. Testis Ces aktivitesi sadece BPA grubunda kontrol grubuna kıyasla önemli düzeyde daha düşüktü (p < 0.001). Testis MDA konsantrasyonu sadece BPA grubunda kontrol grubuna kıyasla daha yüksekti (p < 0.001). İndirgenmiş GSH düzeyi siyah havuç ve BPA+Siyah havuç gruplarında kontrol grubuna kıyasla daha yüksekti (p < 0.05). GST aktivitesi BPA grubunda kontrol grubuna göre daha düşüktü (p < 0.05). Siyah havuç ve BPA + Siyah havuç gruplarında GST aktiviteleri kontrol grubuna kıyasla daha yüksekti (p

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Cite This Article

ŞAHİN, Z., ÖZKAYA, A., YOLOĞLU, E., UÇKUN, M., ŞAHİN, Y., BULMUŞ, Ö. (2022). Evaluating Effects of Black Carrot Extract on Testicular Carboxylesterase Activity and Oxidative Stress Parameters in Rats Exposed to Bisphenol A. Adıyaman Üniversitesi Fen Bilimleri Dergisi, Cilt: 11 - Sayı: 1, 73–86. https://ajindex.com/articles/428418

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