AI Summary & Key Takeaways
AI InsightBlood ureaand creatinine levels were evaluated for nephrotoxicity, and the rats wereimmunohistochemically examined for glomerular pathology.Results: Epithelization was statistically significantly decelerated(p<0.01), diameter of the trachea was statistically significantly larger(p<0.05), and wall thickness of the trachea was significantly thicker inthe group with topical mitomycin-C application compared to the controlgroup (p<0.01).Conclusion: Topically applied mitomycin-C following tracheal surgeryslows down epithelization and, thus, decreases the development ofgranulation tissue and maintains a wider diameter of the trachea.
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Abstract
Background: This study aims to evaluate the effect of mitomycin-Capplied through different drug administration approaches on thedevelopment of granulation tissue in the field of surgery and renalfunctions in rats which underwent tracheal surgery.Methods: Fifty male adult Sprague Dawley rats (weighing mean200 g to 300 g) were divided into five groups. An incision was performedbetween the fifth and sixth cartilage ring of the trachea in all groupsunder anesthesia and the incision was primarily repaired with a 6/0monofilament absorbable suture. A single dose of mitomycin-C 0.5 mgwas applied in the experimental animals appropriate with their assignedgroups as topical, intraperitoneal injection, injection to the wound edges,and through inhalation. No mitomycin-C was administered in one groupwhich was accepted as the control group. Rats were sacrificed four weeksafter surgery and their tracheas were excised subsequently. Tracheal tissuesamples were histopathologically evaluated in terms of epithelization,fibrosis, amount of fibroblasts, angiogenesis, and inflammatory response.Diameter and wall thickness of the tracheas were measured. Blood ureaand creatinine levels were evaluated for nephrotoxicity, and the rats wereimmunohistochemically examined for glomerular pathology.Results: Epithelization was statistically significantly decelerated(p<0.01), diameter of the trachea was statistically significantly larger(p<0.05), and wall thickness of the trachea was significantly thicker inthe group with topical mitomycin-C application compared to the controlgroup (p<0.01).Conclusion: Topically applied mitomycin-C following tracheal surgeryslows down epithelization and, thus, decreases the development ofgranulation tissue and maintains a wider diameter of the trachea.
Özet
Amaç: Bu çalışmada trakea cerrahisi uygulanan sıçanlarda farklı ilaç uygulama yaklaşımları ile uygulanan mitomisin-C’nin cerrahi bölgede granülasyon dokusu gelişimi ve böbrek fonksiyonları üzerine etkisi değerlendirildi. Çalışma planı: Elli erkek erişkin Sprague Dawley sıçan (ağırlık ort. 200 g-300 g) beş gruba ayrıldı. Tüm gruplara anestezi altında trakeanın beşinci ve altıncı kıkırdak halkası arasından kesi yapıldı ve kesi 6/0 monofilaman emilebilir dikiş ile primer olarak onarıldı. Deney hayvanlarına atandıkları topikal, intraperitoneal enjeksiyon, yara kenarlarına enjeksiyon ve inhalasyon yolu gruplarına uygun olarak bir doz 0.5 mg mitomisin-C uygulandı. Kontrol grubu kabul edilen bir gruba mitomisin-C uygulanmadı. Cerrahiden dört hafta sonra sıçanlar sakrifiye edildi ve takiben trakeaları çıkarıldı. Trakea dokuları histopatolojik olarak epitelizasyon, fibrozis, fibroblast miktarı, angiogenezis ve enflamatuar yanıt açısından değerlendirildi. Trakea çapı ve duvar kalınlığı ölçüldü. Kan üre ve kreatinin değerleri nefrotoksisite açısından değerlendirildi ve sıçanlar immunhistokimyasal olarak glomeruler patoloji açısından incelendi. Bulgular: Kontrol grubuna kıyasla topikal mitomisin-C uygulanan grupta epitelizasyon istatistiksel olarak anlamlı derecede yavaşladı (p<0.01), trakea çapı istatistiksel olarak anlamlı derecede daha genişti (p<0.05) ve trakea duvar kalınlığı anlamlı olarak daha kalındı (p<0.01). Sonuç: Trakea cerrahisi sonrası topikal olarak uygulanan mitomisin-C epitelizasyonu yavaşlatarak granülasyon dokusu gelişimini azaltmakta ve trakea çapının daha geniş kalmasını sağlamaktadır.