Journals / Ziraat Fakültesi Dergisi / 2006 / Cilt: 1 - Sayı: 1

Application of Genetic Engineering in Citrus

Turunçgillerde Genetik Mühendisliği Uygulamaları

Pages
74–86
Publication Date
2006-01-31
DOI
—
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AI Summary & Key Takeaways

AI Insight
⚡ TL;DR(Too Long; Didn't Read)

In this study, genetic transformation studies in Citrus were reviewed and its prospects in the near future and its importance for Turkey were discussed.

📌 3 Key Takeaways

🎯
Objective & Hypothesis:As in other plants, application of genetic engineering in Citrus requires availability of genes for desired traits and development of genetic transformation methods.
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Key Findings & Data:Once these conditions are met, traits that can not be integrated into the Citrus genome by conventional breeding could be introduced to citrus by genetic engineering.
💡
Conclusion & Impact:In this study, genetic transformation studies in Citrus were reviewed and its prospects in the near future and its importance for Turkey were discussed.
🔬Design:Review & Systematic Survey
📍Scope:Comprehensive Sample
⚙️Variables:Turunçgiller · genetik mühendisliği · genetik transformasyon

Abstract

As in other plants, application of genetic engineering in Citrus requires availability of genes for desired traits and development of genetic transformation methods. Once these conditions are met, traits that can not be integrated into the Citrus genome by conventional breeding could be introduced to citrus by genetic engineering. Hence, a number of studies were conducted in Citrus and transgenic citrus plants were produced from different types of plant material using various transformation methods in the last decade. Initially, citrus plants were transformed only with reporter genes. As the transformation methods were optimized and genes for agriculturally important traits become availible by cloning from different sources, genetic engineering was applied for developing resistance to diseases, pests and abiotic stresses as well as improving fruit quality in Citrus. As a result, many transgenic plants transformed with these genes were produced and molecular and biological characteristization of these transgenic plants are still underway. In this study, genetic transformation studies in Citrus were reviewed and its prospects in the near future and its importance for Turkey were discussed.

Özet

Diğer bitkilerde olduğu gibi turunçgillerde de genetik mühendisliği uygulamalarının yapılması ve yaygınlaşması, istenilen özellikleri sağlayacak genlerin bulunmasına ve bunları bitkiye aktaracak transformasyon yöntemlerinin geliştirilmesine bağlıdır. Bu iki temel koşulun sağlanmasıyla turunçgillere ıslah çalışmalarıyla kazandırılamayan bazı özellikler genetik mühendisliği yoluyla kazandırılabilir. Bu amaçla turunçgillerde son on yılda çok sayıda çalışma yapılmış olup farklı bitki materyali ve farklı gen aktarım yöntemleri kullanılarak transgenik bitkiler üretilmiştir. Başlangıçta transformasyon çalışmaları sadece raportör genler kullanılarak yapılmıştır. Daha sonra transformasyon yöntemlerinin optimizasyonu ve tarımsal açıdan önemli genlerin çeşitli kaynaklardan klonlanmasıyla turunçgillerde hastalık ve zararlılar yanında abiyotik streslere dayanıklılık ve meyve kalitesinin artırılmasına yönelik genetik mühendisliği uygulamaları da başlamıştır. Bu uygulamalar sonucunda birçok transgenik bitki üretilmiş olup halen bu transgenik bitkilerin moleküler ve biyolojik karakterizasyonları yapılmaktadır. Bu çalışmada dünyada turunçgillerde yapılan genetik transformasyon çalışmaları ve bunların sonuçları derlenerek yakın gelecekteki durumu ve ülkemiz açısından önemi tartışılmıştır.

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Cite This Article

ÇEVİK, B., ÇEVİK, M. Ş. (2006). Application of Genetic Engineering in Citrus. Ziraat Fakültesi Dergisi, Cilt: 1 - Sayı: 1, 74–86. https://ajindex.com/articles/422886

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References (8)

  1. Almeida, W.A.B, Mourão-Filho, F.A.A., Pino, L.E., Boscariol, R.L., Rodríguez A.P.M., Mendes, B.M.J. 2003. Genetic transformation and plant recovery from mature tissues of Citrus sinensis L. Osbeck. Plant Science 164: 203–211.
  2. Bond, J.E. and Roose, M.L. 1998. Agrobacterium-mediated transformation of the commercially important citrus cultivar Washington navel orange. Plant Cell Rep. 18: 229–234.
  3. Boscariol R. L., Almeida, W.A.B., Derbyshire, M.T.V.C., Mourao-Filho, F.A.A. and Mendes, B.M.J. 2003. The use of the PMI/mannose selection system to recover transgenic sweet orange plants (Citrus sinensis L. Osbeck). Plant Cell Rep. 22:122–128.
  4. Ceccardi, T.L., Barthe, G.A. and Derrick, K.S. 1998. A novel protein associated with citrus blight has sequence similarities to expansin. Plant Mol. Biol. 38: 775–783.
  5. Cervera, M., Ortega C., Navarro, A., Navarro, L. and Pena, L. 2000. Generation of transgenic citrus plants with the tolerance-tosalinity gene HAL2 from yeast, J. Hort. Sci. Biotech. 75 26–30.
  6. Cervera, M., Pina, J.A., Juarez, J., Navarro, L. and Pena, L. 1998. Agrobacterium-mediated transformation of citrange: factors affecting transformation and regeneration. Plant Cell Rep. 18: 271–278.
  7. Cevik, B., Lee, R.F., Moore, G.A. and Niblett, C.L. 2000. Genetic Transformation of Citrus paradisi with the RNA-Dependent RNA Polymerase Gene of Citrus Tristeza Closterovirus. In: Proceedings of the 9th International Citrus Congress. (Ed.:L. G. Albrigo),Vol. 1, CREC-UF/IFAS, Lake Alfred, pp. 205. Cevik, B. 2001. Characterization of the RNA-dependent RNA polymerase gene of citrus tristeza closterovirus. Ph.D Thesis. University of Florida, Gainesville, Florida, 138 pp. Cevik, B., Lee, R.F. and Niblett, C.L. 2006. Genetic Transformation of Citrus paradisi with antisense and untranslatable constructs of the RNA-Dependent RNA Polymerase Gene of Citrus Tristeza Closterovirus. T. J. Agric. Forest. In press. Champ, K. 2004. Isolation and characterization of components of low temperature-induced signal transduction pathways in Poncirus trifoliata (L.) Raf. and Citrus paradisi Macf. Ph.D Thesis. University of Florida, Gainesville, Florida,125 pp. Chávez-Vela, N.A., Lucía, I., Chávez-Ortiz, E. And Pérez-Molphe B. 2003. Genetic transformatıon of sour orange using Agrobacterium rhizogenes. Agrociencia. 37: 629-639.
  8. Chiba, H., Uehara, M., Wu, J., Wang, X., Masuyama, R., Suzuki, K., Kanazawa, K. and Ishimi, Y. 2003. Hesperidin, a citrus flavonoid, inhibits bone loss and decreases serum and