Journals / Akademik Gıda / 2018 / Cilt: 16 - Sayı: 1

Lab-On-A-Chip (LOC) Technology Applications in Food Microbiology

Çip-Üstü-Laboratuvar (ÇÜL) Teknolojisinin Gıda Mikrobiyolojisindeki Uygulamaları

Pages
78–87
Publication Date
2022-10-11
DOI
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AI Summary & Key Takeaways

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⚡ TL;DR(Too Long; Didn't Read)

In this study, principles of LOC devices, integrated systems used with polymerase chain reaction (PCR), flow cytometry, biosensors and application of these systems in food microbiology are reviewed.

📌 3 Key Takeaways

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Objective & Hypothesis:Lab-on-a-chip (LOC) systems, which have emerged in the last two decades and used for fluid processing in a micro-channel structure at microliter levels, are alternatives to conventional methods.
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Key Findings & Data:Because of the unique properties of microstructures, LOC systems can make complex analyses more efficient with lower cost, energy and chemical consumption than conventional systems, and they become a valuable option for many applications such as biomedical, food, chemistry, medicine, pharmacy and agriculture in these aspects.
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Conclusion & Impact:In this study, principles of LOC devices, integrated systems used with polymerase chain reaction (PCR), flow cytometry, biosensors and application of these systems in food microbiology are reviewed.
🔬Design:Review & Systematic Survey
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Lab-on-a-chip (LOC) systems, which have emerged in the last two decades and used for fluid processing in a micro-channel structure at microliter levels, are alternatives to conventional methods. Because of the unique properties of microstructures, LOC systems can make complex analyses more efficient with lower cost, energy and chemical consumption than conventional systems, and they become a valuable option for many applications such as biomedical, food, chemistry, medicine, pharmacy and agriculture in these aspects. In addition, these systems are suitable for automation and have a potential to be portable. Especially in some cases of food industry, obtaining analysis results quickly has a great importance for food plants. For this reason, recently developed LOC technology has become important in food industry because of their features such as quick response in microbiological analysis, no requirement for skilled personnel, and small sample needed. In this study, principles of LOC devices, integrated systems used with polymerase chain reaction (PCR), flow cytometry, biosensors and application of these systems in food microbiology are reviewed.

Özet

Son 20 yılda ortaya çıkan mikro-kanal yapılarında mikro-litreler mertebesinde akışkanın proses edilmesi için kullanılan çip-üstü-laboratuvar (ÇÜL) sistemleri konvansiyonel yöntemlere bir alternatif oluşturmaktadır. Mikro-yapıların kendine has özelliklerinden dolayı ÇÜL sistemleri, karmaşık analizleri geleneksel yöntemlerle karşılaştırıldığında daha düşük maliyette, daha düşük enerji, daha düşük kimyasal sarfiyatı ile daha verimli bir şekilde yapabilmekte ve bu yönleriyle birçok alanda (biyomedikal, gıda, kimya, tıp, eczacılık, tarım vb.) çok değerli bir seçenek oluşturmaktadır. Ayrıca bu sistemler otomasyona uygundur ve taşınabilir olma potansiyelleri vardır. Özellikle gıda endüstrisinde bazı durumlarda analiz sonuçlarının hızlı alınabilmesi işletme için büyük önem taşımaktadır. Çip-üstü laboratuvar teknolojisi bu nedenle gıda endüstrisinde özellikle mikrobiyolojik analizlerde hızlı sonuç vermesi, uzman personel gerektirmemesi, az örneğe ihtiyaç duyması vb. özellikleri ile son zamanlarda oldukça önem taşımaktadır. Bu çalışmada, ÇÜL cihazlarının çalışma prensipleri, polimeraz zincir reaksiyonu (PZR), akış sitometrisi ve biyosensörler ile birlikte kullanıldığı entegre sistemler ve bu sistemlerin gıda mikrobiyolojisindeki uygulamaları anlatılmıştır.

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Cite This Article

ÇETİN, B., KIŞLA, D., DÜVEN, G. (2022). Lab-On-A-Chip (LOC) Technology Applications in Food Microbiology. Akademik Gıda, Cilt: 16 - Sayı: 1, 78–87. https://ajindex.com/articles/422881

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References (17)

  1. Wen-Ming, L., Li, L., Li, R., Jian-Chun, W., Qin, T., Xue-Qin, W., Jin-Yi, W., 2012. Diversification of microfluidic chip for applications in cell based bioanalysis. Chinese Journal of Analytical Chemistry 40(1): 24-31
  2. Ürkmez, B., 2014a. Minyatür Laboratuvarlar-2. http://www.acikbilim.com/2014/02/dosyalar/minyatur-laboratuvarlar-2.html, Erişim Tarihi: 21.08.2016
  3. Erickson, D., Li, D., 2004. Integrated microfluidic devices. Analytica Chimica Acta 507: 11-26
  4. Ürkmez, B., 2014b. Minyatür Laboratuvarlar-1. http://www.acikbilim.com/2014/01/dosyalar/minyatur-laboratuvarlar-1.html, Erişim Tarihi: 23.08.2016
  5. Yoon, J., Kim, B., 2012. Lab-on-chip pathogen sensors for food safety. Sensors 12: 10713-10741
  6. Liu, W., Zhu, L., 2005. Environmental microbiology-on-a-chip and its feature impacts. Trends in Biotechnology 23(4): 174-179
  7. Mortari, A., Lorenzelli, L., 2014. Recent sensing technologies for pathogen detection in milk: a review. Biosensors and Bioelectronics 60: 8-21
  8. Sakamoto, C., Yamaguchi, N., Yamada, M., Nagase, H., Seki, M., Nasu, M., 2007. Rapid quantification of bacterial cells in potable water using a simplified microfluidic device. Journal of Microbiological Methods 68: 643-647
  9. Skurtys, O., Aguilera, J., M., 2008. Applications of microfluidic devices in food engineering. Food Biophysics 3: 1-15
  10. Timur, S., 2010. Protein Analitiği Bölüm: Protein Chip’leri, Editörler A., Telefoncu, A. Kılınç, Ege Üniversitesi Basımevi, Bornova, İzmir
  11. Anonymous, 2015. Lab-on-a-chip. http://www.epa.gov/radiation/docs/cleanup/nanotechnology/chapter-3-lab-on-a-chip.pdf, Erişim Rarihi: 21.05.2015
  12. Guo, L., Feng, J., Fang, Z., Xu, J. and Lu, X., 2015. Application of microfluidic ”lab-on-a-chip” for the detection of mycotoxins in foods. Trends in Food Science & Technology 46: 252-263
  13. Anonymous, 2016a. Polymerase Chain Reaction (PCR). http://www.ncbi.nlm.nih.gov/probe/docs/techpcr/, Erişim Tarihi: 30.08.2016
  14. Anonymous, 2016b, Thermo scientific Standard PCR protocol reaction set up
  15. Koop, M.U., de Mello, A.J., Manz, A., 1998. Chemical amplification: Continuous-flow PCR on a chip. Science 280: 1046-1047
  16. Li, Y., Zhang, C., Xing, D., 2011. Integrated microfluidic reverse transcription-polymerase chain reaction for rapid detection of food or waterborn pathogenic rotavirus. Analytical Biochemistry 415: 87-96
  17. Delibato, E., Gattuso, A., Minucci, A., Auricchio, B., De Medici, D., Toti, L., Castagnola, M., Ca