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Journals / Turkish Journal of Hematology / 2016 / Cilt: 33 - Sayı: 2

Regulatory Cells in Patients with Idiopathic Thrombocytopenio Purpura

idiyopatik Trombositopenik Purpura Olgularında Düzenleyici Hücreler

Alev Akyol ERİKÇİ
BÜLENT KARAGÖZ
OĞUZ BİLGİ
Pages
153–155
DOI
—
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AI Summary & Key Takeaways

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⚡ TL;DR(Too Long; Didn't Read)

We conclude that these circulatory cells are not different in ITP, but further studies are needed to explore the putative roles of these regulatory cells

📌 3 Key Takeaways

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Objective & Hypothesis:Objective: Immune thrombocytopenic purpura (ITP) is an immune— mediated bleeding disorder in which platelets are opsonized by autoantibodies and destroyed by an Fc receptor—mediated phagocytosis by the reticuloendothelial system within the spleen.
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Key Findings & Data:CD4+CD25+FOXP3+ regulatoryT [Treg] cells and CD8+CD28— Treg cells have roles in autoimmune diseases.
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Conclusion & Impact:We conclude that these circulatory cells are not different in ITP, but further studies are needed to explore the putative roles of these regulatory cells
🔬Design:Research Article
📍Scope:Comprehensive Sample
⚙️Variables:Domain Parameters

Abstract

Objective: Immune thrombocytopenic purpura (ITP) is an immune— mediated bleeding disorder in which platelets are opsonized by autoantibodies and destroyed by an Fc receptor—mediated phagocytosis by the reticuloendothelial system within the spleen. Autoimmune processes are also considered in the pathogenesis of this disorder. CD4+CD25+FOXP3+ regulatoryT [Treg] cells and CD8+CD28— Treg cells have roles in autoimmune diseases. We investigated these regulatory cells in ITP patients. Materials and Methods: We included 22 ITP patients and 16 age—matched healthy subjects. CD4+CD25+FOXP3+ Treg cells and CD8+CD28- cells were investigated by three—color flow cytometry. The ratios of these cell populations to total lymphocytes were calculated. Statistical analysis was carried outwith the Mann—Whitney test. Results: CD4+CD25+ Treg cells were 9.69i3.700/0 and 12.99i5.580/0 in patients with ITP and controls, respectively. CD4+CD25hİ9hFoxP3+ cells were 27-72i19.740/0 and 27.55i23.980/o in ITP patients and controls, respectively. The percentages of both of these cell types were not statistically significant when compared to the control group. Conclusion: We did not find any differences in ratios of CD4+CD25+FOXP3+ Treg cells or CD8+CD28— cells in lymphocytes between patients and healthy subjects. We conclude that these circulatory cells are not different in ITP, but further studies are needed to explore the putative roles of these regulatory cells

Özet

Amaç: İmmün trombositopenik purpura (İTP) trombositlerin otoantikorlar tarafından opsonize edildiği ve retiküloendotelyal sistem tarafından Fc reseptör aracılı fagositoz ile dalakta yıkıldığı immün kaynaklı bir kanama bozukluğudur. Bu bozukluğun patogenezinde otoimmün süreçler de sorumlu tutulmaktadır. CD4+CD25+Foxp3+ regulatuvar T (Treg) hücreleri ve CD8+CD28- Treg hücreler otoimmün hastalıklarda rol oynamaktadır. Çalışmamızda İTP'li hastalarda bu regülatuvar hücreleri araştırdık. Gereç ve Yöntemler: İTP'li 22 hasta ile yaş uyumlu 16 sağlıklı birey dahil edildi. CD4+CD25+Foxp3+ Treg hücreler ve CD8+CD28- hücreler üç renkli akım sitometri ile çalışıldı. Bu hücre popülasyonunun tüm lenfositlere oranı hesaplanmıştır. İstatiktiksel değerlendirmede MannWhitney U testi kullanılmıştır.Bulgular: CD4+CD25+ Treg hücreler İTP'de ve kontrol grubunda %9,69±3,70 ve %12,99±5,58 saptandılar. CD4+CD25 yüksek FoxP3+ hücreler ise İTP'de ve kontrol grubunda %27,72±19,74 ve %27,55±23,9 olarak saptandı. Her iki hücre tipi de kontrol grubu ile karşılaştırıldığında istatiktiksel olarak anlamlı bulunmamıştır.Sonuç: Lenfositlerdeki CD4+CD25+Foxp3+ Treg hücreler ve CD8+CD28- T hücrelerdeki oranlarında fark bulamadık. Biz çalışmamızda İTP'de dolaşan regulatuvar hücrelerde fark bulamadık ama daha geniş kapsamlı çalışmalara ihtiyaç vardır

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Cite This Article

ERİKÇİ, A. A., KARAGÖZ, B., BİLGİ, O. (2016). Regulatory Cells in Patients with Idiopathic Thrombocytopenio Purpura. Turkish Journal of Hematology, Cilt: 33 - Sayı: 2, 153–155. https://ajindex.com/articles/412490

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